Functional analysis of vitamin D receptor (VDR) using adenovirus vector

Kise, Satoko; Iijima, Ayano; Nagao, Chika; Okada, Tadashi; Mano, Hiroki; Nishikawa, Miyu; Ikushiro, Shinichi; Kanemoto, Yoshitsugu; Kato, Shigeaki; Nakanishi, Teruyuki; Sato, Shigeto; Yasuda, Kaori; Sakaki, Toshiyuki

doi:10.1016/j.jsbmb.2023.106275

Cited by 5 publications

(29 citation statements)

References 29 publications

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“…As described previously, AdVs expressing VDR or each of its mutants appear to be useful for elucidating the detailed mechanism of VDR function in sustaining the hair cycle [20]. Recently, we revealed that VDR(R270L/H301Q) has almost no binding a nity for either 1,25D3 or 25D3.…”

Section: Discussionmentioning

confidence: 84%

“…Recently, we revealed that VDR(R270L/H301Q) has almost no binding a nity for either 1,25D3 or 25D3. In addition, we generated Vdr(R270L/H301Q) rats using genome editing [20]. Although these rats showed abnormal bone formation, no alopecia was observed, suggesting that unliganded VDR plays an important role in hair cycle maintenance.…”

Section: Discussionmentioning

confidence: 99%

“…We constructed rat VDR expressing Ad (VDR-Ad) and GFP expressing Ad (GFP-Ad) using a cosmid cassette [20]. HEK293 cells (ATCC CRL-1573, a human embryonic kidney cell line containing the Ad 5 E1 region; American Type Culture Collection [ATCC], Manassas, VA) were maintained in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).…”

Section: Structure Of Cosmid Cassette and Advmentioning

confidence: 99%

“…The DNA fragment encoding wild type rat VDR, which was modi ed from viewpoint of codon usage without change of amino acid sequence, was chemically synthesized, as previously described [20]. In addition, Kozak sequence and BsrG1 site at 5' terminus, and NsiI site at 3' terminus were added [20]. The plasmid containing Vdr cDNA fragment was cleaved by BsrG1 and NsiI, and after blunting, the resultant fragment was inserted into Swa I site of the cassete cosmid pAxEFwtGit2.…”

Section: Construction Of Adv Expressing Wild Type Of Rat Vdr and Its ...mentioning

confidence: 99%

“…After electrophoresis, gels were electrotransferred onto PVDF membranes. The membranes were incubated in TBS-T containing 5% skim milk, and then incubated with anti-VDR antibody (D2K6, rabbit mAb) (Cell Signaling Technology, Danvers, MA, USA) [20]. The membranes were washed three times with Tris-buffered saline containing 0.05% Tween 20 (TBS-T) and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (Cell Signaling Technology, Danvers, MA, USA).…”

Section: Western Blot Analysismentioning

confidence: 99%

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Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Kise

Iijima

Nagao

et al. 2023

Preprint

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Type II rickets is a hereditary disease caused by a mutation in the vitamin D receptor (VDR) gene. The main symptoms of this disease are bone dysplasia and alopecia. Bone dysplasia can be ameliorated by high calcium intake; however, there is no suitable treatment for alopecia. In this study, we verified whether gene therapy using an adenoviral vector (AdV) had a therapeutic effect on alopecia in Vdr-KO rats. The VDR-expressing AdV was injected into six 7-week-old female Vdr-KO rats (VDR-AdV rats). On the other hand, control-AdV was injected into 7-week-old female rats (control-AdV rats); non-infected Vdr-KO rats (control rats) were also examined. The hair on the backs of the rats was shaved with hair clippers, and VDR-AdV or control-AdV was intradermally injected. Part of the back skin was collected from each rat after AdV administration. Hair follicles were observed using hematoxylin and eosin staining, and VDR expression was examined using immunostaining and western blotting. VDR-AdV rats showed significant VDR expression in the skin, enhanced hair growth, and low cyst formation, whereas control-AdV and non-infected rats did not show any of these effects. These results indicate that gene therapy is useful to treat alopecia associated with type II rickets.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Structure Of Cosmid Cassette and Advmentioning

confidence: 99%

Section: Construction Of Adv Expressing Wild Type Of Rat Vdr and Its ...mentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

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Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Kise

Iijima

Nagao

et al. 2023

Preprint

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Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Kise,

Iijima,

Nagao

et al. 2023

Sci Rep

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Type II rickets is a hereditary disease caused by a mutation in the vitamin D receptor (VDR) gene. The main symptoms of this disease are bone dysplasia and alopecia. Bone dysplasia can be ameliorated by high calcium intake; however, there is no suitable treatment for alopecia. In this study, we verified whether gene therapy using an adenoviral vector (AdV) had a therapeutic effect on alopecia in Vdr-KO rats. The VDR-expressing AdV was injected into six 7-week-old female Vdr-KO rats (VDR-AdV rats). On the other hand, control-AdV was injected into 7-week-old female rats (control-AdV rats); non-infected Vdr-KO rats (control rats) were also examined. The hair on the backs of the rats was shaved with hair clippers, and VDR-AdV or control-AdV was intradermally injected. Part of the back skin was collected from each rat after AdV administration. Hair follicles were observed using hematoxylin and eosin staining, and VDR expression was examined using immunostaining and western blotting. VDR-AdV rats showed significant VDR expression in the skin, enhanced hair growth, and low cyst formation, whereas control-AdV and non-infected rats did not show any of these effects. The effect of VDR-AdV lasted for nearly 60 days. These results indicate that gene therapy using VDR-AdV may be useful to treat alopecia associated with type II rickets, if multiple injections are possible after a sufficient period of time.

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Association Between Trichoscopic Features and Serum Hormone Levels and Vitamin D Concentration in Patients with Androgenetic Alopecia in Eastern China: A Cross-Sectional Study

Chen,

Tao,

Zhu

et al. 2023

CCID

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Purpose Androgenetic alopecia (AGA) is a common dermatological condition, with reported associations between serum vitamin D and sex hormone levels. However, the relationship between these factors and the severity of hair loss remains unclear. Patients and Methods Our cross-sectional study included 310 AGA patients who visited the dermatology clinic and underwent trichoscopy examinations throughout 2021. We collected data on serum sex hormones and 25(OH)D levels during their consultations. TrichoScan analysis was used to characterize and quantify AGA severity based on hair density and vellus hair proportions in the central scalp area, which were then correlated with the collected indicators. Results The study findings reflect the basic demographic characteristics of AGA patients in the Chinese population. We discovered a significant negative correlation between serum SHBG levels and AGA severity in women, but no association was found in men. Serum testosterone, estradiol, dehydroepiandrosterone sulfate, and 25(OH)D levels showed no significant correlation with the severity of androgenetic alopecia, regardless of gender. Conclusion To our knowledge, this is the first study to investigate the relationship between serum sex hormones, vitamin D, and AGA severity using trichoscopic scalp hair features.

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Functional analysis of vitamin D receptor (VDR) using adenovirus vector

Cited by 5 publications

References 29 publications

Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Association Between Trichoscopic Features and Serum Hormone Levels and Vitamin D Concentration in Patients with Androgenetic Alopecia in Eastern China: A Cross-Sectional Study

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