Functional Analysis of V2 Protein of Beet Curly Top Iran Virus

Bahari, Atiyeh; Safaie, Naser; Bejarano, Eduardo R.; Luna, Ana P.; Shams-Bakhsh, Masoud

doi:10.3390/plants11233351

Cited by 3 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, such an experiment only addresses the effect of BCTIV in the reversal of the DNA methylation not in the initiation or maintenance of DNA methylation. In addition to Rep, a recent publication also reported the local VSR activity of BCTIV-V2 [52], which overlapped with the results presented in this manuscript. Although no sequence homology has been established between Becutrovirus-V2 and Begomovirus-AV2, it is worth mentioning that -AV2 was identified as a suppressor with a weaker silencing outcome than P19 [53],…”

Section: Discussionsupporting

confidence: 90%

Beet Curly Top Iran Virus Rep and V2 Suppress Post-Transcriptional Gene Silencing via Distinct Modes of Action

Ebrahimi,

Eini,

Baßler

et al. 2023

Viruses

View full text Add to dashboard Cite

Beet curly top Iran virus (BCTIV) is a yield-limiting geminivirus belonging to the becurtovirus genus. The genome organization of BCTIV is unique such that the complementary strand of BCTIV resembles Mastrevirus, whereas the virion strand organization is similar to the Curtovirus genus. Geminiviruses are known to avoid the plant defense system by suppressing the RNA interference mechanisms both at the transcriptional gene silencing (TGS) and post-transcriptional gene silencing (PTGS) levels. Multiple geminivirus genes have been identified as viral suppressors of RNA silencing (VSR) but VSR activity remains mostly elusive in becurtoviruses. We found that BCTIV-V2 and -Rep could suppress specific Sense-PTGS mechanisms with distinct efficiencies depending on the nature of the silencing inducer and the target gene. Local silencing induced by GFP inverted repeat (IR) could not be suppressed by V2 but was partially reduced by Rep. Accordingly, we documented that Rep but not V2 could suppress systemic silencing induced by GFP-IR. In addition, we showed that the VSR activity of Rep was partly regulated by RNA-dependent RNA Polymerase 6 (RDR6), whereas the VSR activity of V2 was independent of RDR6. Domain mapping for Rep showed that an intact Rep protein was required for the suppression of PTGS. In summary, we showed that BCTIV-Rep and -V2 function as silencing suppressors with distinct modes of action.

show abstract

Section: Discussionsupporting

confidence: 90%

Beet Curly Top Iran Virus Rep and V2 Suppress Post-Transcriptional Gene Silencing via Distinct Modes of Action

Ebrahimi,

Eini,

Baßler

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

“…In addition, V2 caused a mild leaf curling, thickening, and asymmetric leaves, while V1, V3, and C2 had no clear effect on plant phenotype [ 26 ]. In another study, Bahari et al [ 27 ] mutated the BCTIV V2 and concluded that the gene plays a crucial role In viral pathogenicity and systemic movement by suppressing host post-transcriptional gene silencing (PTGS) mechanisms. Similarly, Ebrahimi et al [ 28 ] found that V2 and Rep were able to suppress specific PTGS mechanisms, while BCTIV-V2 could suppress S-PTGS more efficiently than BCTIV-Rep. Luna et al [ 29 ] revealed that V2 from BCTV is able to induce systemic symptoms and necrosis associated with a hypersensitive response, and that this pathogenicity activity is not dependent on its ability to suppress PTGS.…”

Section: Discussionmentioning

confidence: 99%

Development of Highly Efficient Resistance to Beet Curly Top Iran Virus (Becurtovirus) in Sugar Beet (B. vulgaris) via CRISPR/Cas9 System

Yıldırım

Kavas

Küçük

et al. 2023

IJMS

View full text Add to dashboard Cite

Beet Curly Top Iran Virus (BCTIV, Becurtovirus) is a dominant and widespread pathogen responsible for great damage and yield reduction in sugar beet production in the Mediterranean and Middle East. CRISPR-based gene editing is a versatile tool that has been successfully used in plants to improve resistance against many viral pathogens. In this study, the efficiency of gRNA/Cas9 constructs targeting the expressed genes of BCTIV was assessed in sugar beet leaves by their transient expression. Almost all positive control sugar beets revealed systemic infection and severe disease symptoms (90%), with a great biomass reduction (68%) after BCTIV agroinoculation. On the other hand, sugar beets co-agronioculated with BCTIV and gRNA/Cas9 indicated much lower systemic infection (10–55%), disease symptoms and biomass reduction (13–45%). Viral inactivation was also verified by RCA and qPCR assays for gRNA/Cas9 treated sugar beets. PCR-RE digestion and sequencing assays confirmed the gRNA/Cas9-mediated INDEL mutations at the target sites of the BCTIV genome and represented high efficiencies (53–88%), especially for those targeting BCTIV’s movement gene and its overlapping region between capsid and ssDNA regulator genes. A multiplex CRISPR approach was also tested. The most effective four gRNAs targeting all the genes of BCTIV were cloned into a Cas9-containing vector and agroinoculated into virus-infected sugar beet leaves. The results of this multiplex CRISPR system revealed almost complete viral resistance with inhibition of systemic infection and mutant escape. This is the first report of CRSIPR-mediated broad-spectrum resistance against Becurtovirus in sugar beet.

show abstract

“…RNAhybrid identified nine ghr-miRNAs: ghr-miR393, ghr-miR482 (a, b), ghr-miR7486 (a, b), ghr-miR7490, ghr-miR7504a, ghr-miR7510a and ghr-miR7512 at nucleotide positions 611, 581, 849, 670, 694 and 917, respectively (Figure 3D and Table 2) and (Table S3-S4 and File S1). [70][71][72][73]. The ghr-miR7497 targeted the V2 gene at (locus 349) and (loci 155, 459), as identified by the miRanda and psRNATarget algorithms, respectively (Figure 3A and 3C).…”

Section: V1 Encoding Coat Protein (Cp)mentioning

confidence: 98%

Genome-Wide Identification of Cotton MicroRNAs Predicted for Targeting Cotton Leaf Curl Kokhran Virus-Lucknow

Ashraf,

Brown,

Iqbal

et al. 2023

Preprint

View full text Add to dashboard Cite

Cotton leaf curl Kokhran virus (CLCuKoV) (genus, Begomovirus; family, Geminiviridae) is highly infectious, widespread and the most dangerous pathogen of cotton (Gossypium hirsutum L.) that is responsible to a serious disorder, cotton leaf curl disease (CLCuD). Begomoviruses are spread very efficiently by the whitefly Bemisia tabaci cryptic species, causing economic losses to cotton crop, all over the world. The ‘Lucknow’ strain of CLCuKoV has emerged as a divergent isolate that could cause CLCuD. The monopartite ssDNA genome of CLCuKoV-Lu (2.7 Kb) contains six open reading frames (ORFs) that was shown to encode four major proteins. RNA interference (RNAi)-based antiviral innate immunity is a sequence-specific biological phenomenon and a powerful tool to control plant viruses. The present study aims to determine cotton locus-derived microRNAs (ghr-miRNAs) that are identified for targeting the CLCuKoV-Lu ss-DNA-encoded mRNAs using a predictive approach that involves four computational algorithms, miRanda, RNA22, psRNATarget and RNAhybrid. Mature ghr-miRNA sequences (n=80) from allotetraploid upland cotton (2n = 4x = 52) were selected from the miRBase and were tested for alignment with the CLCuKoV-Lu genome. Among the 80 cotton locus-derived ghr-miRNAs evaluated, only one consensus cotton locus-derived ghr-miRNA (ghr-miR2950) was concluded to have effective ghr-miRNA target site at common nucleotide position 82 in the CLCuKoV-Lu genome respectively, using a stringent criterion, identified by all the algorithms used. The miRNA targeting is reliant on base pairing of miRNA-mRNA target pairings. Conservation of the hybridization binding site of the predicted ghr-miR2950 was validated using multiple sequence alignment within all the strains of CLCuKoV. We constructed a regulatory interaction network of miRNA–mRNA to identify novel targets. The efficacy of the predicted miRNAs against CLCuKoV-Lu was evaluated by RNAi-mediated targeted mRNA cleavage. The current investigated miRNA targets provide evidence for the development of CLCuD-resistant cotton plants.

show abstract

Functional Analysis of V2 Protein of Beet Curly Top Iran Virus

Cited by 3 publications

References 55 publications

Beet Curly Top Iran Virus Rep and V2 Suppress Post-Transcriptional Gene Silencing via Distinct Modes of Action

Beet Curly Top Iran Virus Rep and V2 Suppress Post-Transcriptional Gene Silencing via Distinct Modes of Action

Development of Highly Efficient Resistance to Beet Curly Top Iran Virus (Becurtovirus) in Sugar Beet (B. vulgaris) via CRISPR/Cas9 System

Genome-Wide Identification of Cotton MicroRNAs Predicted for Targeting Cotton Leaf Curl Kokhran Virus-Lucknow

Contact Info

Product

Resources

About