Functional analysis of the taproot and fibrous roots of Medicago truncatula: Sucrose and proline catabolism primary response to water deficit

Castañeda, Verónica; Peña, Marlon de la; Azcárate, Lidia; Aranjuelo, Íker; González, Esther M.

doi:10.1016/j.agwat.2018.07.018

Cited by 14 publications

(45 citation statements)

References 67 publications

(72 reference statements)

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“…This is in agreement with the observed proteomic changes in M. truncatula [ 27 ], where drought stress provoked an accumulation of protein degradation and amino acid synthesis-related proteins in roots while decreasing in shoots, as suggested by our enzymatic studies ( Table 5 ). In addition, the increased BCAA levels ( Table S1 ), as previously observed [ 25 ], could be used as alternative electron donors for respiration, reinforcing their role on water-deficit tolerance [ 68 ].…”

Section: Discussionsupporting

confidence: 56%

“…This increase in root strength under No-W could be linked to the increase in alkINV observed in this organ, which could compensate for the decrease in the SuSy activity ( Table 5 ), as observed previously in arabidopsis [ 55 ]. The decrease in root SuSy activity in response to abiotic stress has been already reported for roots of salt-stressed maize [ 56 ] and drought-stressed sorghum [ 57 ] and M. truncatula [ 25 ], being described as one of the main and faster markers of abiotic stress [ 25 ]. In our study, the decrease in root sink strength due to a decrease in both SuSy and INV under salinity stress might indicate a redistribution of assimilates to the shoots rather than to the roots in NaCl-stressed M. truncatula plants.…”

Section: Discussionmentioning

confidence: 86%

“…Many authors have studied the effect of water-deficit stress in leaves [ 23 ] or roots [ 24 , 25 ], but not many have simultaneously addressed the response in both organs [ 26 , 27 ], and even less at the whole-plant level including the phloem sap [ 15 , 19 ], even though the study of a plant as a whole can give us a unique and valuable information and understanding of its response to stress. We hypothesize that the impact of different osmotic treatments frequently applied to simulate water-deficit stress is markedly different at the whole-plant level.…”

Section: Introductionmentioning

confidence: 99%

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Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula

Castañeda

González

2021

IJMS

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Water-deficit stresses such as drought and salinity are the most important factors limiting crop productivity. Hence, understanding the plant responses to these stresses is key for the improvement of their tolerance and yield. In this study M. truncatula plants were subjected to 250 mM NaCl as well as reduced irrigation (No-W) and 250 g/L polyethylene glycol (PEG)-6000 to induce salinity and drought stress, respectively, provoking a drop to −1.7 MPa in leaf water potential. The whole plant physiology and metabolism was explored by characterizing the stress responses at root, phloem sap and leaf organ level. PEG treatment led to some typical responses of plants to drought stress, but in addition to PEG uptake, an important impairment of nutrient uptake and a different regulation of carbon metabolism could be observed compared to No-W plants. No-W plants showed an important redistribution of antioxidants and assimilates to the root tissue, with a distinctive increase in root proline degradation and alkaline invertase activity. On the contrary, salinity provoked an increase in leaf starch and isocitrate dehydrogenase activity, suggesting key roles in the plant response to this stress. Overall, results suggest higher protection of salt-stressed shoots and non-irrigated roots through different mechanisms, including the regulation of proline and carbon metabolism, while discarding PEG as safe mimicker of drought. This raises the need to understand the effect at the whole plant level of the different strategies employed to apply water-deficit stress.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula

Castañeda

González

2021

IJMS

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“…Although an increase in temperature breaks the low‐temperature restriction on plants, warming‐induced drought may restrain the biomass production of some drought‐intolerant species. In general, taproot species have a higher water absorption capacity than fibrous root species because taproot species can absorb water and nutrients from deeper soil layers which are relatively less affected by warming‐induced drought than shallow soils (Castañeda et al., 2019). Our results showed that warming had a negative impact on the biomass production of fibrous root species, K. pygmaea and P. pratensis (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Phenological changes offset the warming effects on biomass production in an alpine meadow on the Qinghai–Tibetan Plateau

Ganjurjav

Gornish

et al. 2020

Journal of Ecology

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Phenology is an important indicator of plant responses to environmental changes and is closely correlated with biomass production. However, how changes in phenological events affect plant biomass production when exposed to changing temperature and precipitation remain unclear. We conducted a 4‐year manipulative experiment of warming and precipitation addition to explore phenology–biomass interactions under climate change in a dry alpine meadow on the central Qinghai–Tibetan Plateau from 2015 to 2018. In dry and warm years, warming delayed phenology and precipitation addition advanced them. Warming decreased the biomass of Kobresia pygmaea in 2018 and the biomass of Poa pratensis in 2015, 2017 and 2018. However, precipitation addition significantly increased the biomass of Poa pratensis and Potentilla multifida in most of the experimental years. Phenological changes regulated the responses of biomass to treatments. Specifically, delay of green up of P. pratensis and delay of withering of K. pygmaea induced by warming can increase biomass production, but it can be offset by the direct negative effects of warming on biomass. Synthesis. Here we show how warming‐induced drought tend to decrease the biomass production of graminoids and the negative effects of warming on the biomass of P. pratensis and K. pygmaea were partially offset by green up postponement and withering postponement respectively. Our results highlights phenology is a crucial regulator for biomass production under climate change. Hence, both direct and indirect effects of warming and precipitation addition on phenology and biomass cannot be ignored when predicting biomass responses to climate change.

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“…Proline has been shown to have a strong correlation with increased drought tolerance (Balestro et al, 2017;Castañeda et al, 2018). However, Mansour and Ali (2017), and Marcos et al (2018) argued that since proline is produced under stressful conditions, its absence or low production by the plant may indicate less stress.…”

Section: Resultsmentioning

confidence: 99%

Agro-biochemical Traits of Sugarcane Varieties Grown in the Brazilian Semi Arid Region

Leal¹,

Rocha²,

Oliveria³

et al. 2019

JAS

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The objective of this research was to evaluate the productive response associate to biochemical indicators and oxidative enzymes activities involved in the water deficit resistance in eight sugarcane varieties (RB951541, RB931011, RB962962, RB867515, RB763710, RB72454, RB863129, and RB92579) grown in the semi-arid regions of Brazil. Compared to all other varieties, RB763710 was superior in the number of stems m-1, mean height, and stem diameter, production of whole plant fresh biomass and stem. When biochemical responses were obtained, all sugarcane varieties had a statistically similar solubility and maturity, regardless of the specific maturity rate of each cultivar. The increase in soluble carbohydrate levels occurred in the most stress-sensitive varieties and the variety RB763710 had the highest proline content. A lower general correlation was observed between the production of fresh biomass of stalks and the enzymatic activity. Among the varieties, RB763710 had the highest enzymatic activities which possibly provided greater tolerance to water stress due to the ability to maintain the redox state in the leaves of plants under water deficit. The study of the adaptation mechanisms of sugarcane against water deficit has contributed to the development and diffusion of genotypes tolerant to rainfed conditions, thus contributing to increased productivity even under adverse conditions, allowing maintenance and optimization of the production chains of sugarcane in rainfed regions.

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Functional analysis of the taproot and fibrous roots of Medicago truncatula: Sucrose and proline catabolism primary response to water deficit

Cited by 14 publications

References 67 publications

Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula

Strategies to Apply Water-Deficit Stress: Similarities and Disparities at the Whole Plant Metabolism Level in Medicago truncatula

Phenological changes offset the warming effects on biomass production in an alpine meadow on the Qinghai–Tibetan Plateau

Agro-biochemical Traits of Sugarcane Varieties Grown in the Brazilian Semi Arid Region

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