Functional analysis of the promoter of the MdFRK2 gene encoding a high-affinity fructokinase in apple (malus × domestica)

Yang, Jingjing; Zhan, Ruiling; Jin, Yuru; Song, Jianyu; Li, Dongxia; An, Guiyang; Li, Mingjun

doi:10.1016/j.scienta.2019.109088

Cited by 7 publications

(5 citation statements)

References 45 publications

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“…The CDS of MdERF4 , MdHDA19 , MdTPL4 were individually cloned into the binary pRI101 vector to generate effector constructs pRI101- MdERF4 , pRI101- MdTPL4 , and pRI101- MdHDA19 ( Li et al, 2016 ). Reporter constructs were generated using the sequences of MdACS3a , A1 , or A3 , which was binding sites of MdHDA19 to MdACS3a , cloned upstream of the GUS reporter gene in the binary vector pBI121 ( Yang et al, 2020 ). For the transient expression assay, the reporter vector and the effector vector were transformed into A. tumefaciens GV3101 and co-infiltrated into N. tabacum leaves as in Yin et al (2010) .…”

Section: Methodsmentioning

confidence: 99%

Ethylene response factor MdERF4 and histone deacetylase MdHDA19 suppress apple fruit ripening through histone deacetylation of ripening-related genes

Han

Wang

et al. 2022

Plant Physiology

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Histone deacetylase enzymes participate in the regulation of many aspects of plant development. However, the genome-level targets of histone deacetylation during apple (Malus domestica) fruit development have not been resolved in detail, and the mechanisms of regulation of such a process are unknown. We previously showed that the complex of ethylene response factor 4 (MdERF4) and the TOPLESS co-repressor (MdTPL4) (MdERF4-MdTPL4) is constitutively active during apple fruit development, but whether this transcriptional repression complex is coupled to chromatin modification is unknown. Here, we show that a histone deacetylase (MdHDA19) is recruited to the MdERF4-MdTPL4 complex, thereby impacting fruit ethylene biosynthesis. Transient suppression of MdHDA19 expression promoted fruit ripening and ethylene production. To identify potential downstream target genes regulated by MdHDA19, we conducted chromatin immunoprecipitation (ChIP) sequencing of H3K9 and ChIP-quantitative PCR assays. We found that MdHDA19 affects ethylene production by facilitating H3K9 deacetylation and forms a complex with MdERF4-MdTPL4 to directly repress MdACS3a expression by decreasing the degree of acetylation. We demonstrate that an early-maturing-specific acetylation H3K9ac peak in MdACS3a and expression of MdACS3a were specifically up-regulated in fruit of an early-maturing, but not a late-maturing, cultivar. We provide evidence that a C-to-G mutation in the EAR motif of MdERF4 reduces the repression of MdACS3a by the MdERF4-MdTPL4-MdHDA19 complex. Taken together, our results reveal that the MdERF4-MdTPL-MdHDA19 repressor complex participates in the epigenetic regulation of apple fruit ripening.

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Section: Methodsmentioning

confidence: 99%

Ethylene response factor MdERF4 and histone deacetylase MdHDA19 suppress apple fruit ripening through histone deacetylation of ripening-related genes

Han

Wang

et al. 2022

Plant Physiology

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“…After 48 h, the leaves were immersed in staining buffer at 37 °C for 24 h and then rinsed with 70% (v/v) ethanol 66 . The other leaves were frozen in liquid nitrogen to analyze GUS activity according to a previously described method 67 .…”

Section: Methodsmentioning

confidence: 99%

Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon

Zhang

Chang

et al. 2021

Horticulture Research

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Although male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production, its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear. Here, a spontaneous male-sterile watermelon mutant, Se18, was reported to have abnormal tapetum development, which resulted in completely aborted pollen grains. Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1 (ClATM1) encodes a basic helix-loop-helix (bHLH) transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional (BIF) domain in Se18 plants. qRT–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther development. The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis. Moreover, ClATM1 was significantly downregulated in the Se18 mutant, displaying a clear dose effect at the transcriptional level. Subsequent dual-luciferase reporter, β-glucuronidase (GUS) activity, and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding. Collectively, ClATM1 is the first male sterility gene cloned from watermelon, and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants.

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“…In our study, genes encoding fructokinase and trehalose 6-phosphate were upregulated in DT. Fructokinase plays an important role in plant tolerance to abiotic stresses, and it can promote the accumulation of osmoprotectants under drought [50]. Trehalose 6-phosphate phosphatase controls the biosynthesis of trehalose [51], which functions as an osmolyte, carbon reserve, transport sugar, stress protectant, and signaling and homeostatic regulator of sucrose in plants [52].…”

Section: Genes Involved In Photosynthesis and Carbon Metabolismmentioning

confidence: 99%

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Wang

et al. 2021

Plant Physiology and Biochemistry

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BackgroundDrought is one of the main abiotic factors that affect alfalfa yield. The identi cation of genes that control this complex trait can provide important insights for alfalfa breeding. However, little is known about how alfalfa responds and adapts to drought stress, particularly in cultivars of differing drought tolerance. ResultsIn this study, the drought-tolerant cultivar Dryland 'DT' and the drought-sensitive cultivar WL343HQ 'DS' were used to characterize leaf and root physiological responses and transcriptional changes in response to water de cit. Under drought stress, Dryland roots (DTR) showed more differentially expressed genes than WL343HQ roots (DSR), whereas WL343HQ leaves (DSL) showed more differentially expressed genes than Dryland leaves (DTL). Many of these genes were involved in stress-related pathways, carbohydrate metabolism, and lignin and wax biosynthesis, which may have improved the drought tolerance of alfalfa. We also observed that several genes related to ABA metabolism, root elongation, peroxidase activity, cell membrane stability, ubiquitination, and genetic processing responded to drought stress in alfalfa. We highlighted several candidate genes, including sucrose synthase, xylan 1,4-betaxylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA reductase, for future studies on drought stress resistance in alfalfa and other plant species. ConclusionsIn summary, our results reveal the unique drought adaptation and resistance characteristics of two alfalfa genotypes. These ndings, which may be valuable for drought resistance breeding, warrant further gene functional analysis to augment currently available information and to clarify the drought stress regulatory mechanisms of alfalfa and other plants. HighlightsWe assembled leaf and root transcriptomes of drought-tolerant and drought-sensitive alfalfa cultivars under control and drought conditions.Under drought, the drought-tolerant cultivar exhibited more differentially expressed metabolites than the drought-sensitive cultivar.Genes associated with carbon metabolism, amino acid metabolism, hormones, and secondary metabolites were differentially expressed under drought stress.The drought-tolerant cultivar exhibited higher expression of genes encoding sucrose synthase, xylan 1,4-beta-xylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA.

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Functional analysis of the promoter of the MdFRK2 gene encoding a high-affinity fructokinase in apple (malus × domestica)

Cited by 7 publications

References 45 publications

Ethylene response factor MdERF4 and histone deacetylase MdHDA19 suppress apple fruit ripening through histone deacetylation of ripening-related genes

Ethylene response factor MdERF4 and histone deacetylase MdHDA19 suppress apple fruit ripening through histone deacetylation of ripening-related genes

Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

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