Functional Analysis of the Pathogenicity-Related Gene VdPR1 in the Vascular Wilt Fungus Verticillium dahliae

Zhang, Yalin; Li, Zhifang; Feng, Zili; Feng, Hongjie; Shí, Yùzhēn; Zhao, Lihong; Zhang, Xiling; Zhu, Heqin

doi:10.1371/journal.pone.0166000

Cited by 12 publications

(13 citation statements)

References 47 publications

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“…As our understanding of the pathogenicity of V. dahliae increases, it appears that a variety of genes are required for virulence towards host plants, including protein kinases (Rauyaree et al ., ; Tian et al ., ; Tzima et al ., ), TFs (Luo et al ., ; Santhanam et al ., ; Sarmiento‐Villamil et al ., ; Tran et al ., ; Xiong et al ., ), effectors (de Jonge et al ., ; Liu et al ., ; Santhanam et al ., ), enzymes involved in plant cell wall degradation (Chen et al ., ; Liu et al ., ; Maruthachalam et al ., ) and other functional genes (Gao et al ., ; Klimes and Dobinson, ; Li et al ., ; Maruthachalam et al ., ; Santhanam et al ., ; Zhang DD et al ., ; Zhang YL et al ., ). In this study, we identified a new pathogenicity and virulence‐related gene encoding a Fungal_trans domain‐containing TF (Figs and ).…”

Section: Discussionmentioning

confidence: 97%

“…Several V. dahliae pathogenicity‐related genes have been identified (Chen et al ., ; de Jonge et al ., ; Liu et al ., ; Maruthachalam et al ., ; Santhanam et al ., ; Zhou et al ., ). In addition, several pathogenicity‐related genes have been identified using T‐DNA insertion mutagenesis (Gao et al ., ; Li et al ., ; Luo et al ., ; Maruthachalam et al ., ; Santhanam et al ., ; Zhang DD et al ., ; Zhang YL et al ., ). Collectively, these studies indicate that the processes associated with pathogenicity are complex, and that a variety of V. dahliae gene products are required for host infection.…”

Section: Introductionmentioning

confidence: 97%

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Verticillium dahliae transcription factor VdFTF1 regulates the expression of multiple secreted virulence factors and is required for full virulence in cotton

Zhang

Gui

Short

et al. 2017

Molecular Plant Pathology

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Fungal transcription factors (TFs) implicated in the regulation of virulence gene expression have been identified in a number of plant pathogens. In Verticillium dahliae, despite its agricultural importance, few regulators of transcription have been characterized. In this study, a T-DNA insertion mutant with significantly reduced virulence towards cotton was identified. The T-DNA was traced to VdFTF1, a gene encoding a TF containing a Fungal_trans domain. Transient expression in onion epidermal cells indicated that VdFTF1 is localized to the nucleus. The VdFTF1-deletion strains displayed normal vegetative growth, mycelial pigmentation and conidial morphology, but exhibited significantly reduced virulence on cotton, suggesting that VdFTF1 is required exclusively for pathogenesis. Comparisons of global transcription patterns of wild-type and VdFTF1-deletion strains indicated that VdFTF1 affected the expression of 802 genes, 233 of which were associated with catalytic processes. These genes encoded 69 potentially secreted proteins, 43 of which contained a carbohydrate enzyme domain known to participate in pathogenesis during infection of cotton. Targeted gene deletion of one VdFTF1-regulated gene resulted in significantly impaired vascular colonization, as measured by quantitative polymerase chain reaction, as well as aggressiveness and symptom severity in cotton. In conclusion, VdFTF1, which encodes a TF containing a Fungal_trans domain, regulates the gene expression of plant cell wall degradation enzymes in V. dahliae, which are required for full virulence on cotton.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Verticillium dahliae transcription factor VdFTF1 regulates the expression of multiple secreted virulence factors and is required for full virulence in cotton

Zhang

Gui

Short

et al. 2017

Molecular Plant Pathology

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“…Verticillium wilt is caused by the systemic colonization of V. dahliae in plant vascular tissues, and plant roots usually serve as the first battleground between V. dahliae and plant host [ 36 ]. The colonization of V. dahliae on cotton roots directly affects the incidence of cotton Verticillium wilt [ 37 ]. Next, the xylem is the second battlefield of V. dahliae and plant host that compete for nutrition [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…The experiment was performed five times. Spore yields at 24, 48, and 72 hpt were estimated with a hemocytometer [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

The Role of a New Compound Micronutrient Multifunctional Fertilizer against Verticillium dahliae on Cotton

et al. 2021

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Verticillium dahliae Kleb., the causal pathogen of vascular wilt, can seriously reduce the yield and quality of many crops, including cotton (Gossypium hirsutum). To control the harm caused by V. dahliae, considering the environmental pollution of chemical fungicides and their residues, the strategy of plant nutrition regulation is becoming increasingly important as an eco-friendly method for disease control. A new compound micronutrient fertilizer (CMF) found in our previous study could reduce the damage of cotton Verticillium wilt and increase yield. However, there is little information about the mode of action of CMF to control this disease. In the present study, we evaluated the role of CMF against V. dahliae and its mechanism of action in vitro and in vivo. In the laboratory tests, we observed that CMF could inhibit hyphal growth, microsclerotia germination, and reduce sporulation of V. dahliae. Further studies revealed that the biomass of V. dahliae in the root and hypocotyl of cotton seedlings treated with CMF were significantly reduced compared with the control, and these results could explain the decline in the disease index of cotton Verticillium wilt. Furthermore, those key genes involved in the phenylpropanoid metabolism pathway, resistance-related genes defense, and nitric oxide signaling pathway were induced in cotton root and hypocotyl tissue when treated with CMF. These results suggest that CMF is a multifaceted micronutrient fertilizer with roles in inhibiting the growth, development, and pathogenicity of V. dahliae and promoting cotton growth.

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“…Fungal strain and cotton variety V. dahliae Vd080 (Zhang et al 2016) which was used in this study was kept on potato dextrose agar (PDA) medium. The mycelia of V. dahliae were obtained from growing the strain in potato dextrose broth (PDB) medium at 28°C, 160 rpm for 12 days.…”

Section: Methodsmentioning

confidence: 99%

A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

Sherzad

Tang

2020

Egypt J Biol Pest Control

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Background Verticillium dahliae is one of the most destructive pathogens in many cotton-growing countries around the globe. There is still the lack of high-resistant upland cotton cultivars against this pathogen, and the usage of fungicides is threatening the environment. Biocontrol is an environment-friendly and a green technique in plant protection. In this regard, the present work was aimed to investigate antagonistic bacterial strains and to introduce them as new bioinoculants against V. dahliae in cotton. Main body of the abstract Numerous bacteria were isolated from cotton roots, and their antagonism was examined against V. dahliae. Strain SZAD2 which was identified as Bacillus velezensis on the bases of 16S rRNA gene analysis had significantly high biocontrol potential both in plate assay and under greenhouse conditions. The biocontrol efficacy in seed treatment was 60.31%, while in the soil drench method, it was 79.19%. Transmission electron microscopy (TEM) analysis revealed that the strain systemically colonized the roots and lived within the intercellular space. Hydrogen peroxide staining exposed that the strain increased plant basal defense response by the accumulation of hydrogen peroxide (H2O2) in both roots and leaves. Furthermore, the bacterization of cotton roots triggered induced systemic resistance (ISR) in the roots and caused the activation of the antioxidant enzymes such as phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), and phenol contents. Moreover, the antifungal extract produced by the strain contained the activity of both cellulase and chitinase, which were able to suppress fungal mycelia both in the presence and absence of cotton. Short conclusions Strain SZAD2 systemically colonized the plant, showed multiple modes of action against the pathogen which played a vital role in V. dahliae control, and is recommended for commercial exploration as a safe alternative bioinoculant instead of hazardous chemical fungicides.

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Functional Analysis of the Pathogenicity-Related Gene VdPR1 in the Vascular Wilt Fungus Verticillium dahliae

Cited by 12 publications

References 47 publications

Verticillium dahliae transcription factor VdFTF1 regulates the expression of multiple secreted virulence factors and is required for full virulence in cotton

Verticillium dahliae transcription factor VdFTF1 regulates the expression of multiple secreted virulence factors and is required for full virulence in cotton

The Role of a New Compound Micronutrient Multifunctional Fertilizer against Verticillium dahliae on Cotton

A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

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