Functional analysis of the G-protein α subunits FGA1 and FGA3 in the banana pathogen Fusarium oxysporum f. sp. cubense

Guo, Lijia; Yang, Yuhua; Yang, Lei; Wang, Feiyang; Wang, Guofen; Jun-sheng, Huang

doi:10.1016/j.pmpp.2016.04.003

Cited by 15 publications

(27 citation statements)

References 33 publications

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“…For example, the conidiation of magB deletion mutant of M. grisea and ∆fga1 mutant of F. oxysporum f. sp. cubense is impaired (Guo et al 2016b;Liu and Dean 1997). A similar phenomenon was also observed in the current study.…”

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationsupporting

confidence: 92%

“…For example, the pathogenicity of the deletion strains M. grisea ∆magB, Botryils cinerea ∆bcg1, and F. oxysporum f. sp. cubense ∆fga1 is markedly reduced (Gronover et al 2001;Guo et al 2016b;Liu and Dean 1997). In the entomopathogenic fungus M. robertsii, MrGpa1 deletion resulted in a marked attenuation of virulence in the G. mellonella model, with an increased LT 50 value (by 63.9%) compared with that of the WT strain.…”

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationmentioning

confidence: 98%

“…In filamentous fungi, genes encoding three G-protein a subunits were reported in N. crassa, F. oxysporum f. sp. cubense, Aspergillus nidulans and M. grisea (Guo et al 2016b;Hicks et al 1997;Ivey et al 1996;Liu and Dean 1997). However, while genes for four G protein a subunits were identified in M. robertsii, two of the open reading frames (ORF) are highly similar (MrGpa2 and MrGpa4).…”

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationmentioning

confidence: 99%

“…This observation was consistent with findings for F. oxysporum f. sp. cubense and N.crassa (Guo et al 2016b;Yang and Borkovich 1999). According to the two cited studies, intracellular cAMP levels are reduced in the Ga i deletion mutants, suggesting that the cAMP pathway might be involved in the response to thermal stress and tolerance of UV irradiation in some fungi.…”

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationmentioning

confidence: 99%

“…It participates in signal transduction pathways linking activated cellsurface receptors with intracellular effectors, including adenylate cyclase and phospholipase through a series of signaling cascades involved in the regulation of physiological and biochemical processes 3 (Chakravorty and Assmann 2018;Ortiz-Urquiza and Keyhani 2015). In fungi, G protein is associated with sexual and asexual reproduction, virulence and response to external signal stimuli (Guo et al 2016b;Ivey et al 1996;Liu and Dean 1997).…”

Section: Introductionmentioning

confidence: 99%

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G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii

Tong

Liu

et al. 2020

Preprint

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G proteins are critical modulators or transducers in various transmembrane signaling systems. They play key roles in numerous biological processes in fungi, including vegetative growth, development of infection-related structures, asexual sporulation, and virulence. However, their function in entomopathogenic fungi remains unclear. Here, we characterized the roles of MrGPA1, a G-protein subunit Gα i , in conidiation, stress resistance, and virulence in Metarhizium robertsii . MrGPA1 was localized in the mitochondria. MrGpa1 deletion resulted in a significant reduction (47%) in the conidiation capacity, and reduced expression of several key conidiation-related genes, including fluG , flbD , brlA , wetA , phiA , and stuA . Further, MrGpa1 disruption resulted in decreased fungal sensitivity to UV irradiation and thermal stress, as determined based on conidial germination of ∆MrGpa1 and wild-type strains. Chemical stress analysis indicated that MrGpa1 contributes to fungal antioxidant capacity and cell wall integrity, but is not involved in antifungal ability and osmotic stress. Importantly, insect bioassays involving (topical inoculation and injection) of Galleria mellonella larvae revealed decreased virulence of ∆MrGpa1 strain after cuticle infection. This was accompanied by decreased rates of appressorium formation and reduced expression of several cuticle penetration-related genes. These observations suggest that MrGpa1 contributes to the regulation of conidiation, UV irradiation, thermal stress response, antioxidant capacity and cell wall integrity in M. robertsii . This gene is also involved in insect cuticle penetration during infection. These findings raise the possibility of designing powerful strategies for genetic improvement of M. robertsii conidiation capacity and virulence for killing pests.

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Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationsupporting

confidence: 92%

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationmentioning

confidence: 98%

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationmentioning

confidence: 99%

Section: Mrgpa1 Plays An Important Role In Insect Cuticle Penetrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii

Tong

Liu

et al. 2020

Preprint

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Insights into intracellular signaling network in Fusarium species

Yang

Huang

et al. 2022

International Journal of Biological Macromolecules

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The G-protein alpha subunit CgGa1 mediates growth, sporulation, penetration and pathogenicity in Colletotrichum gloeosporioides

et al. 2021

Microbial Pathogenesis

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Functional analysis of the G-protein α subunits FGA1 and FGA3 in the banana pathogen Fusarium oxysporum f. sp. cubense

Cited by 15 publications

References 33 publications

G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii

G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii

Insights into intracellular signaling network in Fusarium species

The G-protein alpha subunit CgGa1 mediates growth, sporulation, penetration and pathogenicity in Colletotrichum gloeosporioides

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Functional analysis of the G-protein α subunits FGA1 and FGA3 in the banana pathogen Fusarium oxysporum f. sp. cubense

Cited by 15 publications

References 33 publications

G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii﻿

G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii﻿

Insights into intracellular signaling network in Fusarium species

The G-protein alpha subunit CgGa1 mediates growth, sporulation, penetration and pathogenicity in Colletotrichum gloeosporioides

Contact Info

Product

Resources

About

G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii

G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii