Functional analysis of sweet cherry PavbHLH106 in the regulation of cold stress

Hou, Qiandong; Shen, Tianjiao; Yu, Runrun; Deng, Hong; Wen, Xiaopeng; Qiao, Guang

doi:10.1007/s00299-023-03115-5

Cited by 5 publications

(5 citation statements)

References 65 publications

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“…This imbalance can lead to the production of a large number of reactive oxygen species (ROS) and other harmful metabolites in plants [31]. These substances accumulate to a certain extent, which can damage the cellular structure of plants, increase the permeability of cell membranes, and lead to a large amount of electrolyte leakage [32]. In addition, ROS can also inhibit the activity of biological enzymes, thereby affecting various physiological and metabolic reactions of plants.…”

Section: Discussionmentioning

confidence: 99%

“…In order to determine the localization of VhMYB15 in cells, Agrobacterium containing the VhMYB15-pCAMBIA1300 vector and pCAMBIA1300 vector were injected into 30-dayold tobacco leaves, and the distribution of GFP fluorescence signals was observed under confocal microscopy. DAPI staining was used as a means of assisting localization [32].…”

Section: Vector Construction and Subcellular Localization Of Vhmyb15mentioning

confidence: 99%

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Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thaliana

Han,

Dai,

Chen

et al. 2024

IJMS

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‘Beta’ is a hybrid of Vitis riparia L. and V. labrusca and has a strong ability to adapt to adverse growth environments and is mainly cultivated and used as a resistant rootstock. At present, the most extensively studied MYB TFs are R2R3-type, which have been found to be involved in plant growth, development, and stress response processes. In the present research, VhMYB15, a key transcription factor for abiotic stress tolerance, was screened by bioinformatics in ‘Beta’ rootstock, and its function under salinity and drought stresses was investigated. VhMYB15 was highly expressed in roots and mature leave under salinity and drought stresses. Observing the phenotype and calculating the survival rate of plants, it was found that VhMYB15-overexpressing plants exhibited relatively less yellowing and wilting of leaves and a higher survival rate under salinity and drought stresses. Consistent with the above results, through the determination of stress-related physiological indicators and the expression analysis of stress-related genes (AtSOS2, AtSOS3, AtSOS1, AtNHX1, AtSnRK2.6, AtNCED3, AtP5CS1, and AtCAT1), it was found that transgenic Arabidopsis showed better stress tolerance and stronger adaptability under salinity and drought stresses. Based on the above data, it was preliminarily indicated that VhMYB15 may be a key factor in salinity and drought regulation networks, enhancing the adaptability of ‘Beta’ to adverse environments.

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Section: Discussionmentioning

confidence: 99%

Section: Vector Construction and Subcellular Localization Of Vhmyb15mentioning

confidence: 99%

Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thaliana

Han,

Dai,

Chen

et al. 2024

IJMS

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“…Transcription factors from these families are primarily involved in the CBF-COR cold resistance pathway, directly binding to the promoter regions of CBFs to enhance their expression. For instance, GmNAC20 in soybean ( Hao et al, 2011 ), PbeNAC1 in pear ( Jin et al, 2017 ), and MdNAC104 in apple ( Mei et al, 2023 ), MdCIbHLH1 / MdICE1 , MdICE1L and MdbHLH4 in apple ( Feng et al, 2012 ; An et al, 2021 ; An et al, 2022 ; Yang et al, 2023 ), PavbHLH106 and PavbHLH28 in sweet cherry ( Cao et al, 2023 ; Hou et al, 2023 ), VaWRKY33 in grapevine ( Sun et al, 2019 ), VbWRKY32 in Verbena bonariensis ( Wang M. Q. et al, 2019 ), KoWRKY40 in Kandelia obovate ( Fei et al, 2022 ), CdWRKY2 in bermudagrass ( Huang et al, 2022 ). To date, no research has reported candidate transcription factors from these families involved in peach cold resistance.…”

Section: Discussionmentioning

confidence: 99%

Discovery of candidate genes involved in ethylene biosynthesis and signal transduction pathways related to peach bud cold resistance

Xia,

Yang,

Zhang

et al. 2024

Front. Genet.

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Background: Low temperature pose significant challenges to peach cultivation, causing severe damage to peach buds and restricting production and distribution. Ethylene, an important phytohormone, plays a critical role in enhancing plant cold resistance. Structural genes and transcription factors involved in ethylene biosynthesis and signal transduction pathways are associated with cold resistance. However, no research has specifically addressed their roles in peach cold resistance.Methods: In this study, we aimed for cold-resistance gene discovery in cold-sensitive peach cultivar “21Shiji” (21SJ) and cold-resistance cultivar “Shijizhixing” (SJZX) using RNA-seq and gas chromatography.Results: The findings revealed that under cold stress conditions, ethylene biosynthesis in “SJZX” was significantly induced. Subsequently, a structural gene, PpACO1-1, involved in ethylene biosynthesis in peach buds was significantly upregulated and showed a higher correlation with ethylene release rate. To identify potential transcription factors associated with PpACO1-1 expression and ethylene signal transduction, weighted gene co-expression network analysis was conducted using RNA-seq data. Four transcription factors: PpERF2, PpNAC078, PpWRKY65 and PpbHLH112, were identified.Conclusion: These findings provide valuable theoretical insights for investigating the regulatory mechanisms of peach cold resistance and guiding breeding strategies.

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“…In Prunus avium , cold stress specifically induces the transcription of genes such as PavbHLH28 and PavbHLH106 . Further experiments indicated that the PavbHLH28 and PavbHLH106 enhance the cold resistance by directly binding to the promoters of PavPOD2 and PavACO , respectively. , …”

Section: The Function Of Bhlh Transcription Factors In Abiotic Stress...mentioning

confidence: 95%

“…Further experiments indicated that the PavbHLH28 and PavbHLH106 enhance the cold resistance by directly binding to the promoters of PavPOD2 and PavACO, respectively. 133,217 In summary, there is substantial evidence indicating that the protein stability of bHLH TFs is regulated by post-translational modifications, hormone signaling components, and protein complexes.…”

Section: The Function Of Bhlh Transcription Factors In Abiotic Stress...mentioning

confidence: 99%

Functions of Basic Helix–Loop–Helix (bHLH) Proteins in the Regulation of Plant Responses to Cold, Drought, Salt, and Iron Deficiency: A Comprehensive Review

Lei,

Jiang,

Zhao

et al. 2024

J. Agric. Food Chem.

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Abiotic stresses including cold, drought, salt, and iron deficiency severely impair plant development, crop productivity, and geographic distribution. Several bodies of research have shed light on the pleiotropic functions of BASIC HELIX− LOOP−HELIX (bHLH) proteins in plant responses to these abiotic stresses. In this review, we mention the regulatory roles of bHLH TFs in response to stresses such as cold, drought, salt resistance, and iron deficiency, as well as in enhancing grain yield in plants, especially crops. The bHLH proteins bind to E/G-box motifs in the target promoter and interact with various other factors to form a complex regulatory network. Through this network, they cooperatively activate or repress the transcription of downstream genes, thereby regulating various stress responses. Finally, we present some perspectives for future research focusing on the molecular mechanisms that integrate and coordinate these abiotic stresses. Understanding these molecular mechanisms is crucial for the development of stress-tolerant crops.

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Functional analysis of sweet cherry PavbHLH106 in the regulation of cold stress

Cited by 5 publications

References 65 publications

Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thaliana

Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thaliana

Discovery of candidate genes involved in ethylene biosynthesis and signal transduction pathways related to peach bud cold resistance

Functions of Basic Helix–Loop–Helix (bHLH) Proteins in the Regulation of Plant Responses to Cold, Drought, Salt, and Iron Deficiency: A Comprehensive Review

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