Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (<i>Saccharum</i>) cultivars

Verma, Ashok Kumar; Upadhyay, Santosh Kumar; Verma, Praveen Chandra; Solomon, S.; Singh, S. B.

doi:10.1111/j.1438-8677.2010.00379.x

Cited by 114 publications

(80 citation statements)

References 45 publications

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“…However, the mechanism of how SCMV infection affects SPS protein biosynthesis remains unclear. Since SPS plays a crucial role in sucrose biosynthesis, incorporating with Sucrose Phosphate Phosphatase (SPP) [38,39], the increased activity of SPS would result in a higher sucrose accumulation [40]. In contrast, our result showed a lower SPS activity, but higher sucrose accumulation in infected leaves (Figure 4b).…”

Section: Discussioncontrasting

confidence: 55%

Detection and Response of Sugarcane against the Infection of Sugarcane Mosaic Virus (SCMV) in Indonesia

et al. 2017

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Sugarcane mosaic virus (SCMV) is one among many viruses that infect sugarcane, cause yield loss, and become serious disease agents on sugarcane plantations. Since the morphological symptoms of SCMV are similar to other symptoms caused by Sugarcane streak mosaic virus (SCSMV) or nitrogen deficiency, the detection of SCMV is important through accurate diagnostic-like ELISA or RT-PCR. This research aimed to study the causative mosaic pathogen of SCMV in East Java, Indonesia, including mosaic development. The results showed that the mosaic symptom is present in all sugarcane plantations with 78% and 65% disease incidence and severity, respectively. Moreover, the detection procedure based on an amplification of cDNA of the coat protein gene sequence confirmed that SCMV was the causative agent of mosaic disease on sugarcane. Re-inoculation of healthy sugarcane plants with plant sap from a symptomatic leaf from the field showed similar mosaic or yellowish chlorotic areas on the leaf blade, and appeared on the fourth leaves upward from the inoculation leaf, in addition to showing different levels of peroxidase but not total phenol. Mosaic also correlated with the amount of total chlorophyll. Although Sucrose phosphate synthase (SPS) protein accumulation and activity were at a lower level in infected leaves, sucrose accumulation was at a higher level in the same leaves.

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Section: Discussioncontrasting

confidence: 55%

Detection and Response of Sugarcane against the Infection of Sugarcane Mosaic Virus (SCMV) in Indonesia

et al. 2017

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“…Expression analysis of SAI gene by RT-PCR Expression analysis of SAI gene was performed by following the method as described by Verma et al (2010) for Sucrose phosphate synthase and sucrose synthase genes in sugarcane. Total RNA was isolated from the mature and immature internodes of the different cultivars and cDNAs were synthesized as described above.…”

Section: Extraction and Estimation Of Endogenous Free Sugarsmentioning

confidence: 99%

“…RNA isolation and cDNA synthesis RNA isolation and cDNA synthesis was performed as described by Verma et al (2010) for sugarcane. Total RNA was isolated from 100 mg of plant samples (mature and immature internodes) using total RNA isolation kit (Sigma).…”

Section: Extraction and Estimation Of Endogenous Free Sugarsmentioning

confidence: 99%

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

et al. 2011

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Soluble acid invertase (SAI, EC 3.2.1.26), catalyzes the hydrolysis of sucrose into hexose sugars, and it has been considered a key enzyme for carbohydrate metabolism. In the present study, the activity of SAI enzyme was determined to establish a correlation between the change in transcript levels and enzyme activity in high and low sugar accumulating sugarcane cultivars, in various internodal tissues at different developmental stages. A decrease in SAI activity and transcript levels was observed with age, during all the developmental stages in both the cultivars. A negative correlation between SAI activity and sucrose content was observed in mature and immature internodes; however, there was a positive correlation between SAI activity and content of hexose sugars. These results imply that SAI plays a crucial role in sucrose partitioning in various intermodal tissues in high and low sugar cultivars. In addition to this, the changes in enzyme activity also resulted in changes in transcript level.

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“…Pan et al (2007) reported that four enzymes made great contribution to sucrose accumulation in sugarcane internodes, and SAI, SS and NI were negatively while SPS was positively correlated with sucrose content in cane, respectively. Verma et al (2011) emphasized that SPS activity was positively correlated with sucrose content and negatively correlated with hexose sugars content, but SS activity was negatively correlated with sucrose content and positively correlated with hexose sugars content. Changes of sugar contents due to exogenous ethylene treatment were supported by the changes of different sugar metabolizing enzymes.…”

Section: Enzymes In Carbohydrate Metabolismmentioning

confidence: 99%

Ethylene inhibited sprouting of potato tubers by influencing the carbohydrate metabolism pathway

Hirata

Yang

et al. 2016

J Food Sci Technol

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The aim of this study was to investigate the role of ethylene to control sprouting of potatoes by observing the effect of exogenous ethylene on carbohydrate metabolism and key enzymes. The initial time of potato tuber sprouting and sprouting index were recorded, and rate of respiration, total sugar, total reducing sugar, starch, fructose, glucose, sucrose and the activities of acid invertase (AI), neutral invertase (NI), sucrose synthase (SS), sucrose phosphate synthase (SPS), starch phosphorylase and amylase during sprouting were measured. Exogenous ethylene inhibited sprouting of potato tubers. Moreover, exogenous ethylene increased respiration total sugar, AI activity, SPS activity, SS activity, and reduced sugar and assay activity. Nevertheless, starch, glucose, fructose, NI activity and starch phosphorylase activity showed lower variation. Lower sprouting resulted into potatoes with higher levels of total sugar, total reducing sugar and glucose, and lower level of fructose and sucrose. And sprouting could be inhibited by increasing the activities of SS, SPS and AI by treatment with 199.3 ll L -1 exogenous ethylene. Overall, exogenous ethylene inhibited sprouting of potato tubers by influencing its carbohydrate metabolism.

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Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars

Cited by 114 publications

References 45 publications

Detection and Response of Sugarcane against the Infection of Sugarcane Mosaic Virus (SCMV) in Indonesia

Detection and Response of Sugarcane against the Infection of Sugarcane Mosaic Virus (SCMV) in Indonesia

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

Ethylene inhibited sprouting of potato tubers by influencing the carbohydrate metabolism pathway

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