Functional Analysis of Rice Long-Chain Acyl-CoA Synthetase 9 (OsLACS9) in the Chloroplast Envelope Membrane

Kitajima-Koga, Aya; Baslam, Marouane; Hamada, Y.; Ito, Namiko; Taniuchi, Tomoko; Takamatsu, Takeshi; Oikawa, Kazusato; Kikuchi, Kentaro; Mitsui, Toshiaki

doi:10.3390/ijms21062223

Cited by 11 publications

(7 citation statements)

References 45 publications

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“…9 ). In addition, there were signatures of selection for Long-Chain Acyl-CoA Synthetase ( LCAS ), a gene known for affecting storage oil synthesis and plant height 26 – 28 , and transcriptome analysis showed differential expression in different tissues (Supplementary Fig. 28 ).…”

Section: Resultsmentioning

confidence: 99%

Signatures of selection in recently domesticated macadamia

Lin

Zhang

et al. 2022

Nat Commun

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Macadamia is a high value nut crop that is recently domesticated, ideal for testing the effect of artificial selection. Here, we sequence the genome of Hawaiian cultivar ‘Kau’ and assemble into 794 Mb in 14 pseudo-chromosomes with 37,728 genes. Genome analysis reveals a whole-genome duplication event, occurred 46.8 million years ago. Gene expansions occurred in gene families involves in fatty acid biosynthesis. Gene duplication of MADS-Box transcription factors in proanthocyanidin biosynthesis are relevant for seed coat development. Genome re-sequencing of 112 accessions reveals the origin of Hawaiian cultivars from Mount Bauple in southeast Queensland in Australia. Selective sweeps are detected in macadamia cultivars, including genes involved in fatty acid biosynthesis, seed coat development, and heat stress response. Such strong effects of artificial selection in few generations reveals the genomic basis for ‘one-step operation’ for clonal crop domestication. The knowledge gained could accelerate domestication of new crops from wild species.

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Section: Resultsmentioning

confidence: 99%

Signatures of selection in recently domesticated macadamia

Lin

Zhang

et al. 2022

Nat Commun

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“…Plastids are the places where de novo synthesis of FAs occurs. Several LACS orthologs are known to localize to the plastid envelope ( Figure 2 ), including AtLACS9, HaLACS1 from sunflower ( H. annuus ), and OsLACS9 from rice ( Oryza sativa ) ( Schnurr et al, 2002 ; Aznar-Moreno et al, 2014 ; Jessen et al, 2015 ; Kitajima-Koga et al, 2020 ). They are implicated to function in the activation of de novo synthesized LCFAs in plastids.…”

Section: Subcellular Localizationmentioning

confidence: 99%

Functional Role of Long-Chain Acyl-CoA Synthetases in Plant Development and Stress Responses

2021

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Fatty acids (FAs) play vital roles in plants as components of lipid membranes that demarcate cells and organelles, as sources of stored energy in the form of neutral lipids, and as signaling molecules that elicit plant responses to adverse conditions. The activation of FAs through the formation of acyl-CoA intermediates by acyl-CoA synthetase (ACS) family enzymes is required for their synthesis and degradation. Long-chain ACSs (LACSs) represent a small subgroup of ACS enzymes that specifically convert long-chain or very-long-chain FAs into corresponding thioesters for multiple lipid-associated processes. Alteration of LACS activity often results in pleiotropic phenotypes such as male sterility, organ fusion, aberrant cuticular structure, delayed seed germination, altered seed oil content, and plant capacity to respond to various environmental stresses. This review provides a comprehensive analysis of LACS family enzymes including substrate specificity, tissue-specific expression patterns, and distinct subcellular localization highlighting their specific roles in lipid synthesis and degradation, the effects of altered LACS activity on plant development, the relationship between LACS activity and stress resistance, and the regulation of LACS activity. Finally, we pose several major questions to be addressed, which would advance our current understanding of LACS function in plants.

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“…Breuers et al further research demonstrated the localization of AtLACS9 to the outer envelope membrane [ 41 ]. In rice, the OsLACS9 was located in the chloroplast envelope membrane [ 42 ]. In this study, subcellular localization analysis indicated that the fluorescence signal of the BnLACS9-GFP fusion protein was around the chloroplast but did not overlap with the spontaneous fluorescence signal of the chloroplast and even formed loop structures and thin tubules from the chloroplast (Fig.…”

Section: Discussionmentioning

confidence: 99%

Overexpression the BnLACS9 could increase the chlorophyll and oil content in Brassica napus

Zhu

Zheng

et al. 2023

Biotechnol Biofuels

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Background Chlorophyll is a very important pigment involved in photosynthesis, while plant acyl-CoA biosynthesis is derived from plastid-localized fatty acids (FAs). Until now, the regulation of the acyl-CoA pathway for chlorophyll biosynthesis is still unknown. Results Here, we identified a long-chain acyl-CoA synthetase (LACS) gene BnLACS9 from Brassica napus. BnLACS9 complemented a LACS-deficient yeast strain YB525, which indicated that BnLACS9 has the LACS function. BnLACS9 was localized in the chloroplast envelope membrane, while mainly expressed in young leaves and flowers. Overexpression of BnLACS9 in Nicotiana benthamiana resulted in an increase in total CoA and MGDG content. In B. napus with overexpression of BnLACS9, the number of chloroplast grana lamellae and the chlorophyll content, as well as the MGDG and DGDG contents, increased compared to wild type. The net photosynthetic rate, dry weight of the entire plant and oil content of seeds increased significantly, accompanied by an increase in chlorophyll content. Transcriptome analysis revealed that overexpression of BnLACS9 improved the pathway of acyl-CoA biosynthesis and further improved the enzymes in the glycolipid synthesis pathway, while acyl-CoA was the substrate for glycolipid synthesis. The increased glycolipids, especially MGDG and DGDG, accelerated the formation of the chloroplast grana lamellae, which increased the number of chloroplast thylakoid grana lamella and further lead to increased chlorophyll content. Conclusions In the present study, we demonstrated that BnLACS9 played a crucial role in glycolipids and chlorophyll biosynthesis in B. napus. The results also provide a new direction and theoretical basis for the improvement of the agronomic traits of plants.

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Functional Analysis of Rice Long-Chain Acyl-CoA Synthetase 9 (OsLACS9) in the Chloroplast Envelope Membrane

Cited by 11 publications

References 45 publications

Signatures of selection in recently domesticated macadamia

Signatures of selection in recently domesticated macadamia

Functional Role of Long-Chain Acyl-CoA Synthetases in Plant Development and Stress Responses

Overexpression the BnLACS9 could increase the chlorophyll and oil content in Brassica napus

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