Functional analysis of receptor-like kinases in monocots and dicots

Morillo, Santiago A.; Tax, Frans E.

doi:10.1016/j.pbi.2006.07.009

Cited by 199 publications

(158 citation statements)

References 71 publications

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“…The Arabidopsis genome encodes >200 predicted LRR-RLKs, most of which have unknown functions (Morillo and Tax, 2006). We identified two highly similar LRR-RLKs (82% amino acid identity) ( Figure 1A; see Supplemental Figure 1 online) that when both disrupted caused a swollen-root phenotype (Figures 1 and 2).…”

Section: Disruption Of Fei1 and Fei2 Alters Cell Expansionmentioning

confidence: 98%

“…The RLKs are a large, diverse family of transmembrane signaling elements in plants, only a few of which have been functionally characterized (Morillo and Tax, 2006). The Arabidopsis protein THE1, which belongs to the Cr RLK1L (for Catharanthus roseus protein kinase1-like) subfamily, has been hypothesized to sense cell wall integrity (Hé maty et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

et al. 2008

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The plant cell wall is a dynamic structure that changes in response to developmental and environmental cues through poorly understood signaling pathways. We identified two Leu-rich repeat receptor-like kinases in Arabidopsis thaliana that play a role in regulating cell wall function. Mutations in these FEI1 and FEI2 genes (named for the Chinese word for fat) disrupt anisotropic expansion and the synthesis of cell wall polymers and act additively with inhibitors or mutations disrupting cellulose biosynthesis. While FEI1 is an active protein kinase, a kinase-inactive version of FEI1 was able to fully complement the fei1 fei2 mutant. The expansion defect in fei1 fei2 roots was suppressed by inhibition of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, an enzyme that converts Ado-Met to ACC in ethylene biosynthesis, but not by disruption of the ethylene response pathway. Furthermore, the FEI proteins interact directly with ACC synthase. These results suggest that the FEI proteins define a novel signaling pathway that regulates cell wall function, likely via an ACC-mediated signal.

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Section: Disruption Of Fei1 and Fei2 Alters Cell Expansionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

et al. 2008

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“…Main correlations were observed with genes coding for proteins related to regulatory processes (Fig. 7), a broad category including (1) proteins involved in hormone biosynthesis and signaling, (2) proteins involved in redox regulation, (3) transcription factors of several families, (4) proteins involved in posttranslational modification of protein by folding or proteolysis, and (5) the huge group of protein kinases that have diverse functional roles (Wang et al, 2003;Krupa et al, 2006;Morillo and Tax, 2006). Most of these correlations can be placed within a general regulatory network ( Fig.…”

Section: Metabolite and Regulatory Gene Network Reveal Regulatory Humentioning

confidence: 99%

“…Since these belong to huge protein families with a wide range of functions (Kerk et al, 2002;Wang et al, 2003;Krupa et al, 2006;Morillo and Tax, 2006), they might control many aspects of expanding fruit growth and metabolism, ranging from signaling (e.g. receptor-like kinases; Morillo and Tax, 2006) to cell wall modifications (e.g.…”

Section: Developmental Regulations In Expanding Fruit Tissuesmentioning

confidence: 99%

Gene and Metabolite Regulatory Network Analysis of Early Developing Fruit Tissues Highlights New Candidate Genes for the Control of Tomato Fruit Composition and Development

et al. 2009

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Variations in early fruit development and composition may have major impacts on the taste and the overall quality of ripe tomato (Solanum lycopersicum) fruit. To get insights into the networks involved in these coordinated processes and to identify key regulatory genes, we explored the transcriptional and metabolic changes in expanding tomato fruit tissues using multivariate analysis and gene-metabolite correlation networks. To this end, we demonstrated and took advantage of the existence of clear structural and compositional differences between expanding mesocarp and locular tissue during fruit development (12-35 d postanthesis). Transcriptome and metabolome analyses were carried out with tomato microarrays and analytical methods including proton nuclear magnetic resonance and liquid chromatography-mass spectrometry, respectively. Pairwise comparisons of metabolite contents and gene expression profiles detected up to 37 direct gene-metabolite correlations involving regulatory genes (e.g. the correlations between glutamine, bZIP, and MYB transcription factors). Correlation network analyses revealed the existence of major hub genes correlated with 10 or more regulatory transcripts and embedded in a large regulatory network. This approach proved to be a valuable strategy for identifying specific subsets of genes implicated in key processes of fruit development and metabolism, which are therefore potential targets for genetic improvement of tomato fruit quality.

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“…RLKs have a common molecular structure consisting of a C-terminal cytoplasmic Ser/Thr protein kinase domain connected by a single-pass transmembrane motif to different types of N-terminal ectodomains facing extracytoplasmic compartments. Members of the RLK family play fundamental roles for cellular response programs regulating cell growth, morphogenesis, fertilization, abscission, plant defense, and interaction with symbionts (3)(4)(5)(6)(7). Nevertheless, the attribute "receptor-like" holds for most of the RLKs that still remain orphan with respect to their biological functions and their potential regulatory ligands.…”

mentioning

confidence: 99%

Arabidopsis thaliana Pattern Recognition Receptors for Bacterial Elongation Factor Tu and Flagellin Can Be Combined to Form Functional Chimeric Receptors

Albert

Jehle

Mueller

et al. 2010

Journal of Biological Chemistry

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The receptor kinase EFR of Arabidopsis thaliana detects the microbe-associated molecular pattern elf18, a peptide that represents the N terminus of bacterial elongation factor Tu. Here, we tested subdomains of EFR for their importance in receptor function. Receptor-like kinases (RLKs) 2 form the biggest family of surface receptors in higher plants. Based on genomic sequence information, more than 600 genes in Arabidopsis thaliana and more than 1,000 genes in rice are predicted to encode RLKs (1, 2). RLKs have a common molecular structure consisting of a C-terminal cytoplasmic Ser/Thr protein kinase domain connected by a single-pass transmembrane motif to different types of N-terminal ectodomains facing extracytoplasmic compartments. Members of the RLK family play fundamental roles for cellular response programs regulating cell growth, morphogenesis, fertilization, abscission, plant defense, and interaction with symbionts (3-7). Nevertheless, the attribute "receptor-like" holds for most of the RLKs that still remain orphan with respect to their biological functions and their potential regulatory ligands. Concerning functional aspects of ligand interaction and transmembrane activation, the receptor kinase BRI1, functioning as the receptor for the growth hormone brassinolide, has been most thoroughly studied (8 -10). Thereby, the ligand interaction site could be localized to a small subdomain within the ectodomain of BRI1 (11), and activation was found to involve a ligand-dependent complex formation with a second receptor kinase termed BAK1 for BRI1-associated receptor kinase 1 (9, 10). Transient expression of tagged versions of EFR and EFR lacking its cytoplasmic domain in leaves ofSeveral RLKs have been assigned roles in recognition of pathogen attack. Examples for RLKs that function as pattern recognition receptors for identified ligands include XA21 from rice (12), FLS2 (13), EFR (14), and CERK1 (15) detecting microbe-associated molecular patterns (MAMPs), and AtPEPR1 (16) detecting wound-related, endogenous danger signals. Upon activation with their respective ligands, FLS2, EFR, and AtPEPR1 seem to form heteromeric complexes with the co-receptor BAK1. Thus, intriguingly, BAK1 appears to function in the transmembrane signaling of plant RLKs with very different signal output programs (17)(18)(19)(20).The receptor kinase EFR from A. thaliana responds to bacterial elongation factor Tu (EF-Tu) with the induction of defense and increased resistance (14, 21). Mutant plants lacking this perception system show increased susceptibility to infection by Agrobacterium tumefaciens. The epitope that acts as MAMP was identified as the acetylated N terminus of EF-Tu. Synthetic peptides like elf18 and elf26 which represent the N terminus of EF-Tu with at least 18 amino acids are fully active as MAMPs and stimulate responses at subnanomolar concentrations. A radiolabeled derivative of elf26 was used to demonstrate specific, high affinity binding sites in tissues expressing EFR. In affinity cross-linking experiments this radioli...

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Functional analysis of receptor-like kinases in monocots and dicots

Cited by 199 publications

References 71 publications

Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

Gene and Metabolite Regulatory Network Analysis of Early Developing Fruit Tissues Highlights New Candidate Genes for the Control of Tomato Fruit Composition and Development

Arabidopsis thaliana Pattern Recognition Receptors for Bacterial Elongation Factor Tu and Flagellin Can Be Combined to Form Functional Chimeric Receptors

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