Functional analysis of pilQ gene in Xanthomanas oryzae pv. oryzae, bacterial blight pathogen of rice

Lim, Seon-Hwa; So, B. H.; Wang, Jichun; Song, Eun-Seong; Park, Youngjin; Lee, Byoung-Moo; Kang, Hee Wan

doi:10.1007/s12275-007-0173-9

Cited by 30 publications

(30 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To quantitate biofilm formation in Xoo , we used the polyvinyl chloride microplate method [32]. Xoo strains were cultured in PS broth media overnight and then diluted to concentration 5×10 5 CFU/ml in minimal media.…”

Section: Methodsmentioning

confidence: 99%

Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium

et al. 2011

View full text Add to dashboard Cite

The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axYS22, derived from the Ax21 (activator of XA21-mediated immunity) protein. The conservation of Ax21 in all sequenced Xanthomonas spp. and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in Xanthomonas oryzae pv. oryzae. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the Xoo RaxH histidine kinase serves as the bacterial receptor for Ax21. This work establishes a critical role for small protein-mediated QS in a Gram-negative bacterium.

show abstract

Section: Methodsmentioning

confidence: 99%

Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium

et al. 2011

View full text Add to dashboard Cite

show abstract

“…This seems to be the case for S. symbiotica CWBI-2.3 T . Nevertheless, it cannot be excluded that S. symbiotica use other kinds of motility such as twitching since we identified PilQ (CDS55476.1), a secretin that is essential for the biogenesis of type IV pili and that can be involved in twitching motility, adhesion to host epithelial cells and in protein secretion (Lim et al, 2008). …”

Section: Resultsmentioning

confidence: 99%

Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

Renoz

Champagne

Degand

et al. 2017

PeerJ

View full text Add to dashboard Cite

Symbiotic bacteria are common in insects and can affect various aspects of their hosts' biology. Although the effects of insect symbionts have been clarified for various insect symbiosis models, due to the difficulty of cultivating them in vitro, there is still limited knowledge available on the molecular features that drive symbiosis. Serratia symbiotica is one of the most common symbionts found in aphids. The recent findings of freeliving strains that are considered as nascent partners of aphids provide the opportunity to examine the molecular mechanisms that a symbiont can deploy at the early stages of the symbiosis (i.e., symbiotic factors). In this work, a proteomic approach was used to establish a comprehensive proteome map of the free-living S. symbiotica strain CWBI-2.3 T . Most of the 720 proteins identified are related to housekeeping or primary metabolism. Of these, 76 were identified as candidate proteins possibly promoting host colonization. Our results provide strong evidence that S. symbiotica CWBI-2.3 T is well-armed for invading insect host tissues, and suggest that certain molecular features usually harbored by pathogenic bacteria are no longer present. This comprehensive proteome map provides a series of candidate genes for further studies to understand the molecular cross-talk between insects and symbiotic bacteria.

show abstract

“…Type IV pili are bacterial major virulence factors supporting adhesion, surface motility, and gene transfer [34-36]. The role of type IV pili genes in biofilm formation and virulence of phytopathogenic bacteria has been largely studied in the vascular pathogens Ralstonia solanacearum and Xylella fastidiosa [37-39] and, most recently, in Xoo , Xac , and Xcc [35,40,41]. Type IV in Xoc virulence increased with the presence of two pilY1 insertion mutants [42].…”

Section: Resultsmentioning

confidence: 99%

In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1

et al. 2010

View full text Add to dashboard Cite

BackgroundBacterial leaf blight causes significant yield losses in rice crops throughout Asia and Africa. Although both the Asian and African strains of the pathogen, Xanthomonas oryzae pv. oryzae (Xoo), induce similar symptoms, they are nevertheless genetically different, with the African strains being more closely related to the Asian X. oryzae pv. oryzicola (Xoc).ResultsChanges in gene expression of the African Xoo strain MAI1 in the susceptible rice cultivar Nipponbare were profiled, using an SSH Xoo DNA microarray. Microarray hybridization was performed comparing bacteria recovered from plant tissues at 1, 3, and 6 days after inoculation (dai) with bacteria grown in vitro. A total of 710 bacterial genes were found to be differentially expressed, with 407 up-regulated and 303 down-regulated. Expression profiling indicated that less than 20% of the 710 bacterial transcripts were induced in the first 24 h after inoculation, whereas 63% were differentially expressed at 6 dai. The 710 differentially expressed genes were one-end sequenced. 535 sequences were obtained from which 147 non-redundant sequences were identified. Differentially expressed genes were related to metabolism, secretion and transport, pathogen adherence to plant tissues, plant cell-wall degradation, IS elements, and virulence. In addition, various other genes encoding proteins with unknown function or showing no similarity to other proteins were also induced. The Xoo MAI1 non-redundant set of sequences was compared against several X. oryzae genomes, revealing a specific group of genes that was present only in MAI1. Numerous IS elements were also found to be differentially expressed. Quantitative real-time PCR confirmed 86% of the identified profile on a set of 14 genes selected according to the microarray analysis.ConclusionsThis is the first report to compare the expression of Xoo genes in planta across different time points during infection. This work shows that as-yet-unidentified and potentially new virulence factors are appearing in an emerging African pathogen. It also confirms that African Xoo strains do differ from their Asian counterparts, even at the transcriptional level.

show abstract

Functional analysis of pilQ gene in Xanthomanas oryzae pv. oryzae, bacterial blight pathogen of rice

Cited by 30 publications

References 23 publications

Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium

Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium

Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1

Contact Info

Product

Resources

About