Functional Analysis of Natriuretic Peptide Receptors in the Bladder of the Toad, Bufo marinus

Meier, Stuart; Donald, John A.

doi:10.1006/gcen.2001.7761

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…The differences in results could be due to a difference in response of channels other than low-conductance, highly sodium-selective ENaC [indeed, our laboratory has previously described a nonselective cation channel in lung cells that is regulated quite differently than ENaC and whose activity may be increased by NO (17,20)]. Some of the differences, even when the channels are ENaC, might be due to the off-target effects of 8-Br-cAMP which, besides activating PKG, also alters the activity of phosphodiesterases, thereby altering levels of all cyclic nucleotides in cells (27). Alternatively, the responses might be attributed to the differential expression of different PKG isoforms in different epithelial cells.…”

Section: Resultsmentioning

confidence: 93%

ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling

Guo

Alli

Eaton

et al. 2013

American Journal of Physiology-Renal Physiology

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Epithelial sodium channels (ENaCs) located at the apical membrane of polarized epithelial cells are regulated by the second messenger guanosine 3',5'-cyclic monophosphate (cGMP). The mechanism for this regulation has not been completely characterized. Guanylyl cyclases synthesize cGMP in response to various intracellular and extracellular signals. We investigated the regulation of ENaC activity by natriuretic peptide-dependent activation of guanylyl cyclases in Xenopus 2F3 cells. Confocal microscopy studies show natriuretic peptide receptors (NPRs), including those coupled to guanylyl cyclases, are expressed at the apical membrane of 2F3 cells. Single-channel patch-clamp studies using 2F3 cells revealed that atrial natriuretic peptide (ANP) or 8-(4-chlorophenylthio)-cGMP, but not C-type natriuretic peptide or cANP, decreased the open probability of ENaC. This suggests that NPR-A, but not NPR-B or NPR-C, is involved in the natriuretic peptide-mediated regulation of ENaC activity. Also, it is likely that a signaling pathway involving cGMP and nitric oxide (NO) are involved in this mechanism, since inhibitors of soluble guanylyl cyclase, protein kinase G, inducible NO synthase, or an NO scavenger blocked or reduced the effect of ANP on ENaC activity.

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Section: Resultsmentioning

confidence: 93%

ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling

Guo

Alli

Eaton

et al. 2013

American Journal of Physiology-Renal Physiology

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“…This was an important finding, considering the previously limited data concerning NP control of amphibian renal function (Donald and Trajanovska 2006). In the cane toad bladder, messenger ribonucleic acid (mRNA) for both NP receptor-A and C receptor types was expressed, possibly reflecting the terrestriality of cane toads compared to other frogs in which ANP binding sites appeared to be absent in the bladder (Meier and Donald 2002). However, ANP had no effect on water permeability or vascular resistance of perfused toad bladders so the role of NP receptors in the toad urinary bladder remains unclear.…”

Section: Osmoregulationmentioning

confidence: 78%

Endocrinology of osmoregulation and thermoregulation of Australian desert tetrapods: A historical perspective

Cooper

2017

General and Comparative Endocrinology

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Many Australian tetrapods inhabit desert environments characterised by low productivity, unpredictable rainfall, high temperatures and high incident solar radiation. Maintaining a homeostatic milieu intérieur by osmoregulation and thermoregulation are two physiological challenges faced by tetrapods in deserts, and the endocrine system plays an important role in regulating these processes. There is a considerable body of work examining the osmoregulatory role of antidiuretic hormones for Australian amphibians, reptiles and mammals, with particular contributions concerning their role and function for wild, free-living animals in arid environments. The osmoregulatory role of the natriuretic peptide system has received some attention, while the role of adrenal corticosteroids has been more thoroughly investigated for reptiles and marsupials. The endocrinology of thermoregulation has not received similar attention. Reptiles are best-studied, with research examining the influence of arginine vasotocin and melatonin on body temperature, the role of prostaglandins in heart rate hysteresis and the effect of melanocyte-stimulating hormone on skin reflectivity. Australian mammals have been under-utilised in studies examining the regulation, development and evolution of endothermy, and there is little information concerning the endocrinology of thermoregulation for desert species. There is a paucity of data concerning the endocrinology of osmoregulation and thermoregulation for Australian desert birds. Studies of Australian desert fauna have made substantial contributions to endocrinology, but there is considerable scope for further research. A co-ordinated approach to examine arid-habitat adaptations of the endocrine system in an environmental and evolutionary context would be of particular value.

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Comparative aspects of natriuretic peptide physiology in non-mammalian vertebrates: a review

Toop

Donald

2004

Journal of Comparative Physiology B: Biochemical, Systemic, and

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The natriuretic peptide system is a complex family of peptides and receptors that is primarily linked to the maintenance of osmotic and cardiovascular homeostasis. A natriuretic peptide system is present in each vertebrate class but there are varying degrees of complexity in the system. In agnathans and chondrichthyians, only one natriuretic peptide has been identified, while new data has revealed that multiple types of natriuretic peptides are present in bony fish. However, it seems in tetrapods that there has been a reduction in the number of natriuretic peptide genes, such that only three natriuretic peptides are present in mammals. The peptides act via a family of guanylyl cyclase receptors to generate the second messenger cGMP, which mediates a range of physiological effects at key targets such as the gills, kidney and the cardiovascular system. This review summarises the current knowledge of the natriuretic peptide system in non-mammalian vertebrates and discusses the physiological actions of the peptides.

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Functional Analysis of Natriuretic Peptide Receptors in the Bladder of the Toad, Bufo marinus

Cited by 8 publications

References 28 publications

ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling

ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling

Endocrinology of osmoregulation and thermoregulation of Australian desert tetrapods: A historical perspective

Comparative aspects of natriuretic peptide physiology in non-mammalian vertebrates: a review

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