Functional analysis of membrane vesicles of Listeria monocytogenes suggests a possible role in virulence and physiological stress response

Karthikeyan, Raman; Gayathri, Pratapa; Gunasekaran, Paramasamy; Jagannadham, Medicharla V.; Rajendhran, Jeyaprakash

doi:10.1016/j.micpath.2020.104076

Cited by 9 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibiotics introduce a new challenge for bacteria in an infection, and we now see bEVs acting as ‘decoys’ for antibiotics directed at membrane-bound targets [ 37 ], carrying enzymes that degrade antibiotics in the surrounding milieu [ 38 , 39 ], or enabling horizontal gene transfer (HGT) of antimicrobial resistance genes to susceptible bacterial cells [ 40 ]. A summary of the pathogenic diversity of bEVs from various bacteria is presented in Table 1 .…”

Section: Bacterial Evs In Infectionmentioning

confidence: 99%

The complex, bidirectional role of extracellular vesicles in infection

White

Dauros-Singorenko

Hong

et al. 2021

Biochemical Society Transactions

View full text Add to dashboard Cite

Cells from all domains of life release extracellular vesicles (EVs), packages that carry a cargo of molecules that participate in communication, co-ordination of population behaviours, virulence and immune response mechanisms. Mammalian EVs play an increasingly recognised role to fight infection, yet may also be commandeered to disseminate pathogens and enhance infection. EVs released by bacterial pathogens may deliver toxins to host cells, signalling molecules and new DNA to other bacteria, and act as decoys, protecting infecting bacteria from immune killing. In this review, we explore the role of EVs in infection from the perspective of both the pathogen and host, and highlight their importance in the host/pathogen relationship. We highlight proposed strategies for EVs in therapeutics, and call attention to areas where existing knowledge and evidence is lacking.

show abstract

Section: Bacterial Evs In Infectionmentioning

confidence: 99%

The complex, bidirectional role of extracellular vesicles in infection

White

Dauros-Singorenko

Hong

et al. 2021

Biochemical Society Transactions

View full text Add to dashboard Cite

show abstract

“…MVs are produced by various Gram-positive bacteria, including Staphylococcus aureus, Streptococcus pneumoniae, Mycobacterium tuberculosis, Lactobacillus, Bacillus subtilis, Bacillus anthracis, Listeria monocytogenes , and Clostridium perfringens ( Jiang et al., 2014 ; White et al., 2018 ; Choi et al., 2019 ; Karthikeyan et al., 2020 ; Abe et al., 2021 ; Bitto et al., 2021 ; Mehanny et al., 2022 ). Their diameters range between 20 and 500 nm ( Brown et al., 2015 ).…”

Section: Formation Of Gram-positive Bacteria Mvsmentioning

confidence: 99%

An update on our understanding of Gram-positive bacterial membrane vesicles: discovery, functions, and applications

Xu,

Xie,

Liu

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are nano-sized particles released from cells into the extracellular environment, and are separated from eukaryotic cells, bacteria, and other organisms with cellular structures. EVs alter cell communication by delivering their contents and performing various functions depending on their cargo and release into certain environments or other cells. The cell walls of Gram-positive bacteria have a thick peptidoglycan layer and were previously thought to be unable to produce EVs. However, recent studies have demonstrated that Gram-positive bacterial EVs are crucial for health and disease. In this review, we have summarized the formation, composition, and characteristics of the contents, resistance to external stress, participation in immune regulation, and other functions of Gram-positive bacterial EVs, as well as their application in clinical diagnosis and treatment, to provide a new perspective to further our understanding of Gram-positive bacterial EVs.

show abstract

“…In addition to broth culture, L. monocytogenes also release EVs in infected macrophages, and they have a crucial role in bacterial survival in the host (Vdovikova et al, 2017;Coelho et al, 2019). Proteomic, lipidomic and metabolomic analyses of L. monocytogenes-derived EVs have found these EVs to carry the majority of known Listeria virulence proteins, including the pore-forming hemolysin LLO and phosphatidylinositol-specific phospholipase C (PI-PLC, encoded by plcA) (Lee et al, 2013;Coelho et al, 2019;Karthikeyan et al, 2020Karthikeyan et al, , 2019. L. monocytogenes-derived EVs induce significant cytotoxicity in host Caco-2 cells that is likely due to the presence of LLO and other toxins (Karthikeyan et al, 2020).…”

Section: Evs Released By L Monocytogenesmentioning

confidence: 99%

“…Proteomic, lipidomic and metabolomic analyses of L. monocytogenes-derived EVs have found these EVs to carry the majority of known Listeria virulence proteins, including the pore-forming hemolysin LLO and phosphatidylinositol-specific phospholipase C (PI-PLC, encoded by plcA) (Lee et al, 2013;Coelho et al, 2019;Karthikeyan et al, 2020Karthikeyan et al, , 2019. L. monocytogenes-derived EVs induce significant cytotoxicity in host Caco-2 cells that is likely due to the presence of LLO and other toxins (Karthikeyan et al, 2020). Although LLO is a major virulence factor for Listeria pathogenesis, it can also activate antibacterial pathways that are detrimental for bacterial survival in host cells, such as autophagy (Meyer-Morse et al, 2010).…”

Section: Evs Released By L Monocytogenesmentioning

confidence: 99%

Bacteria- and host-derived extracellular vesicles – two sides of the same coin?

Schorey

Cheng

McManus

2021

Journal of Cell Science

View full text Add to dashboard Cite

Intracellular bacterial pathogens spend portions of their life cycle both inside and outside host cells. While in these two distinct environments, they release or shed bacterial components, including virulence factors that promote their survival and replication. Some of these components are released through extracellular vesicles, which are either derived from the bacteria themselves or from the host cells. Bacteria- and host-derived vesicles have been studied almost exclusively in isolation from each other, with little discussion of the other type of secreted vesicles, despite the fact that both are generated during an in vivo infection and both are likely play a role in bacterial pathogenesis and host immunity. In this Review, we aim to bridge this gap and discuss what we know of bacterial membrane vesicles in their generation and composition. We will compare and contrast this with the composition of host-derived vesicles with regard to bacterial components. We will also compare host cell responses to the different vesicles, with a focus on how these vesicles modulate the immune response, using Mycobacterium, Listeria and Salmonella as specific examples for these comparisons.

show abstract

Functional analysis of membrane vesicles of Listeria monocytogenes suggests a possible role in virulence and physiological stress response

Cited by 9 publications

References 27 publications

The complex, bidirectional role of extracellular vesicles in infection

The complex, bidirectional role of extracellular vesicles in infection

An update on our understanding of Gram-positive bacterial membrane vesicles: discovery, functions, and applications

Bacteria- and host-derived extracellular vesicles – two sides of the same coin?

Contact Info

Product

Resources

About