Functional analysis of CYP6AE68, a cytochrome P450 gene associated with indoxacarb resistance in Spodoptera litura (Lepidoptera: Noctuidae)

Hou, Wen-Tao; Staehelin, Christian; Elzaki, Mohammed Esmail Abdalla; Hafeez, Muhammad; Luo, Yu-Sen; Wang, Ruilong

doi:10.1016/j.pestbp.2021.104946

Cited by 29 publications

(26 citation statements)

References 44 publications

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“…The amino acid sequences of CYP6AE43 and CYP321A7 contain various shared conserved motifs including heme-binding predicted substrate recognition sites, suggesting that the enzyme is functional. These results are in line with previous findings ( Guo et al, 2015 ; Hou et al, 2021 ).…”

Section: Discussionsupporting

confidence: 94%

“…These findings were consistent with previous findings that some of the CYP321 subfamily genes, as well as the CYP6 and CYP9 subfamily genes, may be involved in mechanisms of detoxication or regulation of insecticides. These findings elucidated P450 functions on insect biology and physiology, and that the midgut and fat body tissue of insects are considered important as detoxification organs ( Giraudo et al, 2015 ; Hou et al, 2021 ). It has been previously reported that the development of insecticide resistance by enhancing metabolic detoxification enzymes in various insects is closely related to the elevated expression of P450 genes and subsequent increases in P450 protein levels ( Riveron et al, 2013 ; Xu et al, 2018 ).…”

Section: Discussionmentioning

confidence: 90%

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Down-Regulation of P450 Genes Enhances Susceptibility to Indoxacarb and Alters Physiology and Development of Fall Armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae)

Hafeez

Ullah

et al. 2022

Front. Physiol.

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The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a pest of many important crops globally. Effective control is challenging, with the pest exhibiting resistance to different synthetic pesticides across various groups. However, the mechanisms employed by resistant insects for overexpression of relevant detoxification genes remain unclear. The activity of detoxification enzymes was investigated in this study. Additionally, using RNA interference (RNAi), a functional analysis was completed of two P450s genes in an indoxacarb resistant population of fall armyworms. Elevated resistance levels (resistance ratio = 31.37-fold) in indoxacarb-selected populations of FAW were observed after 14 generations. The qRT-PCR showed higher expression of two cytochrome P450 genes, CYP321A7 and CYP6AE43, in this selected population compared to the control population. RNAi was applied to knock down the P450 dsCYP321A7 and dsCYP6AE43 genes in the FAW larvae. Droplet feeding of the dsRNAs (CYP321A7 and CYP6AE43) via an artificial diet significantly increased mortality rates in the indoxacarb treated population. A shorter larval developmental time of FAW was detected in all dsRNAs-fed larvae. Correspondingly, larval mass was reduced by dsRNAs in indoxacarb resistant populations of fall armyworm. Larval feeding assays demonstrate that dsRNAs targeting, specifically of CYP321A7 and CYP6AE43 enzymes, could be a beneficial technique in the management of indoxacarb resistant populations. Further study on the potential use of dsRNA and its application should be conducted in efforts to counter the development of resistance in FAW against various insecticides in the field.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 90%

Down-Regulation of P450 Genes Enhances Susceptibility to Indoxacarb and Alters Physiology and Development of Fall Armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae)

Hafeez

Ullah

et al. 2022

Front. Physiol.

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“…30 P450-mediated metabolism has already been found to be involved in resistance to indoxacarb in Spodoptera litura and clothianidin in N. lugens. 28,33 The elevated P450 enzyme activities conferring resistance often correlate with overexpression of specific P450 genes. 28,33−35 Shi et al found that the overexpression of six P450 genes was associated with fenpropathrin resistance in Tetranychus cinnabarinus.…”

Section: ■ Discussionmentioning

confidence: 99%

“…The knockdown of CYP6AE68 mediated by RNAi in S. litura increased the mortality of larvae exposed to indoxacarb. 33 In P. xylostella, downregulation of CYP6BG1 gene expression markedly reduced P450 activity and increased larval susceptibility to chlorantraniliprole. 22 RNAi-induced silencing of CYP6ER1 increased the susceptibility of N. lugens to clothianidin.…”

Section: ■ Discussionmentioning

confidence: 99%

Overexpression of Multiple Cytochrome P450 Genes Conferring Clothianidin Resistance in Bradysia odoriphaga

Chunni

Liu

et al. 2022

J. Agric. Food Chem.

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Cytochrome P450 monooxygenases (P450s) play important roles in the detoxification metabolism of xenobiotics and are involved in the resistance of insects to many insecticides. In this study, piperonyl butoxide (PBO), an inhibitor of P450 enzyme activity, significantly increased the toxicity of clothianidin in the clothianidin-resistant (CL-R) population of Bradysia odoriphaga. The enzyme activity of P450 in the CL-R population was significantly higher than that in the SS population. Furthermore, four P450 genes were found to be significantly overexpressed in the CL-R population. Tissue-specific expression analysis indicates that CYP9J57, CYP3828A1, CYP6SX1, and CYP6QE1 were most highly expressed in the midgut and/or Malpighian tubules. After exposure to LC 30 of clothianidin, the expression levels of the four P450 genes were significantly upregulated. The RNAi-mediated knockdown of CYP9J57, CYP3828A1, and CYP6QE1 significantly increased the susceptibility of B. odoriphaga to clothianidin. These results suggest that P450 genes are involved in clothianidin resistance in B. odoriphaga. This provides a better understanding of P450-mediated clothianidin resistance in B. odoriphaga and will contribute to the management of insect resistance to insecticides.

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“…Ulat grayak menyerang daun tanaman dan karakteristik dari kerusakannya berupa daun tanaman yang berlubang (Silalahi et al,2019). Serangan larva ulat grayak dapat merusak daun hingga terlihat tulang daun (Gu et al, 2015;Hou et al, 2021). Hama belalang (Oxya spp.)…”

Section: Pendahuluanunclassified

Respon Ketahanan Tanaman Caisim (Brassica Juncea L.) terhadap Intensitas Serangan Hama dan Penyakit setelah Pemberian Pupuk Kandang Kambing dan Pupuk Hayati Provibio®

Panunggul¹,

Widarawati

Sitanini³

et al. 2022

Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati

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Caisim (Brassica juncea L.) merupakan sayuran yang hasil produk berupa daun segar. Kerusakan tanaman caisim dapat menurunkan nilai jual. Hama belalang (Oxya spp.), ulat pemakan daun, penyakit bercak daun pada (Brassica juncea L.) merupakan hama yang merusak daun tanaman caisim. Penelitian bertujuan mempelajari respon ketahanan pada caisim serangan hama dan penyakit setelah pemberian pupuk kandang dan pupuk hayati Provibio. Metode yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan 2 faktor. Faktor pertama adalah pupuk kambing (P0 = 0 ton/ha (kontrol), P1 = 5 ton-1 per polibag, P2 = 15 ton-1 per polibag) dan faktor kedua adalah pupuk hayati (D0 = 0 mL/polibag (kontrol), D1 = 5 mL/polibag, dan D2 = 10 mL/polibag). Hasil penelitian menunjukkan bahwa pemberian pupuk kandang kambing tidak menunjukkan peningkatan ketahanan yang nyata terhadap intensitas serangan belalang, ulat pemakan daun dan penyakit bercak daun tanaman caisim. Aplikasi pupuk hayati Provibio belum mampu menurunkan hama belalang secara optimal dengan dosis 5 mL, serta belum mampu memberikan ketahanan terhadap ulat daun dan penyakit bercak daun tanaman caisim.

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Functional analysis of CYP6AE68, a cytochrome P450 gene associated with indoxacarb resistance in Spodoptera litura (Lepidoptera: Noctuidae)

Cited by 29 publications

References 44 publications

Down-Regulation of P450 Genes Enhances Susceptibility to Indoxacarb and Alters Physiology and Development of Fall Armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae)

Down-Regulation of P450 Genes Enhances Susceptibility to Indoxacarb and Alters Physiology and Development of Fall Armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae)

Overexpression of Multiple Cytochrome P450 Genes Conferring Clothianidin Resistance in Bradysia odoriphaga

Respon Ketahanan Tanaman Caisim (Brassica Juncea L.) terhadap Intensitas Serangan Hama dan Penyakit setelah Pemberian Pupuk Kandang Kambing dan Pupuk Hayati Provibio®

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