Functional analysis of cis-regulatory regions within the dioxin-inducible CYP1A promoter/enhancer region from zebrafish (Danio rerio)

ZeRuth, Gary; Pollenz, Richard S.

doi:10.1016/j.cbi.2007.07.003

Cited by 32 publications

(34 citation statements)

References 47 publications

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“…10,000 base pairs upstream of the start site and the entire length of the gene were examined for putative zebrafish AREs (TGA(G/C)nnnTC [57]) and XREs (KNGCGTG [58]). Additional searches were carried out for Nfe2 binding sites [59] determined from ChIP-Seq studies.…”

Section: Methodsmentioning

confidence: 99%

Developmental Expression of the Nfe2-Related Factor (Nrf) Transcription Factor Family in the Zebrafish, Danio rerio

et al. 2013

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Transcription factors in the CNC-bZIP family (NFE2, NRF1, NRF2 and NRF3) regulate genes with a wide range of functions in response to both physiological and exogenous signals, including those indicating changes in cellular redox status. Given their role in helping to maintain cellular homeostasis, it is imperative to understand the expression, regulation, and function of CNC-bZIP genes during embryonic development. We explored the expression and function of six nrf genes (nfe2, nrf1a, nrf1b, nrf2a, nrf2b, and nrf3) using zebrafish embryos as a model system. Analysis by microarray and quantitative RT-PCR showed that genes in the nrf family were expressed throughout development from oocytes to larvae. The spatial expression of nrf3 suggested a role in regulating the development of the brain, brachia and pectoral fins. Knock-down by morpholino anti-sense oligonucleotides suggested that none of the genes were necessary for embryonic viability, but nfe2 was required for proper cellular organization in the pneumatic duct and subsequent swim bladder function, as well as for proper formation of the otic vesicles. nrf genes were induced by the oxidant tert-butylhydroperoxide, and some of this response was regulated through family members Nrf2a and Nrf2b. Our results provide a foundation for understanding the role of nrf genes in normal development and in regulating the response to oxidative stress in vertebrate embryos.

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Section: Methodsmentioning

confidence: 99%

Developmental Expression of the Nfe2-Related Factor (Nrf) Transcription Factor Family in the Zebrafish, Danio rerio

et al. 2013

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“…Putative dioxin response elements (DREs) were sought in the promoter regions of the chicken, turkey, and mallard CYP1C genes, using the sequence 5′-(T/G)NGCGTG-3′ [26], [27]. Within 10 kb upstream from the start codons of the CYP1C genes two putative DREs were found in chicken (at −458 and −1671 bp) and one was found in turkey (at −9193 bp).…”

Section: Resultsmentioning

confidence: 99%

“…In addition to AHR affinity of the inducer, the level of CYP1 induction depends on the number of functional DREs in the gene promoters, epigenetic factors, interaction of the AHR with nuclear receptors (e.g., estrogen receptor), cofactors, the AHR repressor, etc. [26], [51], [52], [53], [54], [55]. Because the CYP1 s are likely to be expressed in different cell types, it would be informative to study cell-specific induction of the CYP1 s, and CYP1C1 in particular, in chicken.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 1 Genes in Birds: Evolutionary Relationships and Transcription Profiles in Chicken and Japanese Quail Embryos

et al. 2011

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BackgroundCytochrome P450 1 (CYP1) genes are biomarkers for aryl hydrocarbon receptor (AHR) agonists and may be involved in some of their toxic effects. CYP1s other than the CYP1As are poorly studied in birds. Here we characterize avian CYP1B and CYP1C genes and the expression of the identified CYP1 genes and AHR1, comparing basal and induced levels in chicken and quail embryos.Methodology/Principal FindingsWe cloned cDNAs of chicken CYP1C1 and quail CYP1B1 and AHR1. CYP1Cs occur in several bird genomes, but we found no CYP1C gene in quail. The CYP1C genomic region is highly conserved among vertebrates. This region also shares some synteny with the CYP1B region, consistent with CYP1B and CYP1C genes deriving from duplication of a common ancestor gene. Real-time RT-PCR analyses revealed similar tissue distribution patterns for CYP1A4, CYP1A5, CYP1B1, and AHR1 mRNA in chicken and quail embryos, with the highest basal expression of the CYP1As in liver, and of CYP1B1 in eye, brain, and heart. Chicken CYP1C1 mRNA levels were appreciable in eye and heart but relatively low in other organs. Basal transcript levels of the CYP1As were higher in quail than in chicken, while CYP1B1 levels were similar in the two species. 3,3′,4,5,5′-Pentachlorobiphenyl induced all CYP1s in chicken; in quail a 1000-fold higher dose induced the CYP1As, but not CYP1B1.Conclusions/SignificanceThe apparent absence of CYP1C1 in quail, and weak expression and induction of CYP1C1 in chicken suggest that CYP1Cs have diminishing roles in tetrapods; similar tissue expression suggests that such roles may be met by CYP1B1. Tissue distribution of CYP1B and CYP1C transcripts in birds resembles that previously found in zebrafish, suggesting that these genes serve similar functions in diverse vertebrates. Determining CYP1 catalytic functions in different species should indicate the evolving roles of these duplicated genes in physiological and toxicological processes.

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“…To construct the 3XRE-luc reporter, the following single-strand oligonucleotide (oligo) containing three repeated core sequences of zfXRE (bold) (Zeruth and Pollenz, 2007) was designed: 5′-CGGGGTACCGCTAAAGCATCGCGTGCAAAGGTGT GCTAAAGCATCGCGTGCAAAGGTGTGCTAAAGCATCGCGTGCAAAGGTGT-CCGCTCGAG-3′. The oligo and its complementary strand were synthesized by Tech Dragon Limited, Hong Kong.…”

Section: A Zebrafish Pxr Ligand Binding Domain (Lbd) Construct (Zfpxrmentioning

confidence: 99%

BDE-99, but not BDE-47, is a transient aryl hydrocarbon receptor agonist in zebrafish liver cells

Yang

Zhu

Chan

2016

Toxicology and Applied Pharmacology

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Functional analysis of cis-regulatory regions within the dioxin-inducible CYP1A promoter/enhancer region from zebrafish (Danio rerio)

Cited by 32 publications

References 47 publications

Developmental Expression of the Nfe2-Related Factor (Nrf) Transcription Factor Family in the Zebrafish, Danio rerio

Developmental Expression of the Nfe2-Related Factor (Nrf) Transcription Factor Family in the Zebrafish, Danio rerio

Cytochrome P450 1 Genes in Birds: Evolutionary Relationships and Transcription Profiles in Chicken and Japanese Quail Embryos

BDE-99, but not BDE-47, is a transient aryl hydrocarbon receptor agonist in zebrafish liver cells

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