Abstract:To understand the immunity determinants of bacteriophage cf, we applied DNA shuffling to mutate the intergenic region (IG) for isolation of possible cf immunity mutants. After superinfection screening, an immunity mutant cf-m3 was obtained with a 106-109 fold greater superinfection ability compared with wild type cf. Nine mutations were characterized, four located within the coding region for a hypothetical repressor, PT gene (ORF165), and five located upstream of the PT coding region. To establish whether the… Show more
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