Functional analysis of a grape WRKY30 gene in drought resistance

Zhu, Dan; Che, Yongmei; Xiao, Peilian; Hou, Lixia; Guo, Yang; Liu, Xin

doi:10.1007/s11240-017-1341-1

Cited by 23 publications

(12 citation statements)

References 32 publications

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“…The soluble sugar content, as an osmotic regulator, plays an important role in the resistance of plants to drought stress. Zhu et al examined the effect of VvWRKY30 overexpression on the accumulation of soluble sugars under drought stress and found that soluble sugar content increased in both transgenic and WT Arabidopsis plants, with the increase most pronounced in transgenic plants [44]. Ma et al [45] overexpressed TaWRKY146 in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

“…This effect was can be measured in tolerant genotypes, although the effect is most prominent in sensitive genotypes, such as those of wheat [48]. Zhu et al [44] overexpressed VvWRKY30 in grape and found that after 7 d exposure to 300 mM mannitol the root growth of both the transgenic and WT plants were inhibited, although the effect was greatest in the WT plants. However, Arabidopsis plants overexpressing VvWRKY30 that were grown on basal MS medium without any stress treatment had no significant differences in root growth from that of WT plants.…”

Section: Discussionmentioning

confidence: 99%

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Meta-analysis of the effects of overexpression of WRKY transcription factors on plant responses to drought stress

et al. 2019

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Background The tryptophan-arginine-lysine-tyrosine (WRKY) transcription factors play important roles in plants, allowing them to adapt to environmental conditions that are not normally conducive to plant growth; in particular, drought. There has been extensive research on WRKY transcription factors and the effects of their overexpression in plants on resistance to drought stress. However, due to the materials (the type and species of donor and receptor, promoters) and treatments (the type and time of stress) used, different and often confounding results have been obtained between studies. Meta-analysis is a powerful statistical tool that can be used to summarize results from numerous independent experiments on the same research topic while accounting for variability across experiments. Results We carried out a meta-analysis of 16 measured parameters that affect drought resistance in plants overexpressing WRKY transcription factors and wild-type plants. We found that only one of these parameters was significantly different between transgenic and wild-type plants under drought and control conditions at a 95% confidence interval ( p = 0.000, p = 0.009, respectively). Eleven of the sixteen parameters were obviously different in WRKY transgenic plants under drought and control conditions (SV, p = 0.023, SSC, p = 0.000, SOD, p = 0.012, SFW, p = 0.000, RL, p = 0.016, Pro, p = 0.000, POD, p = 0.027, MDA, p = 0.000, H 2 O 2 , p = 0.003, EL, p = 0.000, CHC, p = 0.000, respectively), seven of the eleven obviously different parameters showed positive effect (SSC, SOD, Pro, POD, MDA, H 2 O 2 , EL), four of them revealed negative effect (SV, SFW, RL, CHC). Conclusion We have found that only one of these parameters was significantly different between transgenic and wild-type plants under drought and control conditions respectively, at a 95% confidence interval. And eleven of sixteen parameters showed obviously different of WRKY-overexpressed plants under different conditions (water-stressed and normal), suggesting that WRKY transcription factors play an important role in plant responses to drought stress. These findings also provide a theoretical basis for further study of the role of WRKY transcription factors in the regulation of plant responses to environmental stress. Electronic supplementary material The online version of this article (10.1186/s12863-019-0766-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Meta-analysis of the effects of overexpression of WRKY transcription factors on plant responses to drought stress

et al. 2019

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“…Soluble sugar content was determined using a previously published method ( Zhu et al, 2018 ). One gram of fresh leaf tissue was reduced to small pieces and then transferred to a test tube with 15 ml of distilled water.…”

Section: Methodsmentioning

confidence: 99%

VvBAP1 Is Involved in Cold Tolerance in Vitis vinifera L.

et al. 2018

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The majority of commercial grape cultivars originate from the European grape. While these cultivars have excellent organoleptic qualities, they suffer from a relatively poor tolerance to the cold experienced during winter, resulting in significant damage to grapevines. Thus, low temperature is one of the bottlenecks that restrict the further growth of the grape industry. Research on the mechanism of cold tolerance in grapes is therefore very important. BON association protein 1 (BAP1) is a recently discovered phospholipid-binding protein. In Arabidopsis, the expression of AtBAP1 can be regulated via low temperature; however, the function of BAP1 in the grapevine has not been reported. The VvBAP1 gene was cloned in our previous studies in grapes, and bioinformatics analysis showed that it harbors the conservative calcium-dependent C2 protein domain. However, little is known about its function and underlying mechanism. In this study, cold treatment was applied to the cold-resistant grape varieties ‘F-242’ and ‘Zuoyouhong’ as well as to the cold-sensitive grape varieties ‘Cabernet Sauvignon’ and ‘Chardonnay.’ The expression level of VvBAP1 in the cold-resistant varieties was significantly higher than in the cold-sensitive varieties, indicating that VvBAP1 could be associated with the cold response processes in the grapevine. Using the cold-resistant grape variety ‘F-242’ as material, with the 4°C and CaCl2 treatment, the relative expression of VvBAP1 was determined via qRT-PCR. Both low temperature and low-temperature signal Ca2+ induced VvBAP1 expression. In addition, the VvBAP1 gene was cloned and transferred into Arabidopsis to generate VvBAP1 overexpressing plants. Biochemical assays and gene expression analyses were conducted on plants subjected to low temperature treatments (4 and -8°C). The obtained results showed that the activities of superoxide dismutase and peroxidase in these transgenic plants were higher than those in wild type (WT) plants, and that cell membrane permeability and malondialdehyde content were both lower compared to WT plants. Furthermore, the content of soluble sugars and the expression levels of sugar-metabolizing related genes, such as BAM4-7, SS4, and G6PD5, were significantly higher than those of WT plants. Furthermore, the expression of low temperature response signal genes, including CBF1, CBF3, COR15a, COR6.6, COR27, and KIN1, were also enhanced. In summary, these results showed that VvBAP1 could strengthen the cold resistance in the grapevine through adjusting and controlling the sugar content and activating antioxidant enzyme activity.

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“…MYB108 has been found to regulate responses to biotic and abiotic stresses including drought [99,100,101]. WRKY30 has been shown that positively regulate drought tolerance [102,103]. NAC29 also enhanced salt and drought tolerance in transgenic Arabidopsis [104].…”

Section: Gene Regulatory Networkmentioning

confidence: 99%

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Baghery

Kazemitabar

Dehestani

et al. 2020

Preprint

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Background: Drought is one of the most common environmental stresses affecting crops yield and quality. Sesame is an important oilseed crop that most likely faces drought during its growth due to growing in semi-arid and arid areas. Plants responses to drought controlled by regulatory mechanisms. Despite this importance, there is little information about Sesame regulatory mechanisms against drought stress. Results: 458 drought-related genes were identified using comprehensive RNA-seq data analysis of two susceptible and tolerant sesame genotypes under drought stress. These drought-responsive genes were included secondary metabolites biosynthesis-related Like F3H, sucrose biosynthesis-related like SUS2, transporters like SUC2, and protectives like LEA and HSP families. Interactions between identified genes and regulators including TFs and miRNAs were predicted using bioinformatics tools and related regulatory gene networks were constructed. Key regulators and relations of Sesame under drought stress were detected by network analysis. TFs belonged to DREB (DREB2D), MYB (MYB63), ZFP (TFIIIA), bZIP (bZIP16), bHLH (PIF1), WRKY (WRKY30) and NAC (NAC29) families were found among key regulators. mRNAs like miR399, miR169, miR156, miR5685, miR529, miR395, miR396, and miR172 also found as key drought regulators. Furthermore, a total of 117 TFs and 133 miRNAs that might be involved in drought stress were identified with this approach. Conclusions: Most of the identified TFs and almost all of the miRNAs are introduced for the first time as potential regulators of drought response in Sesame. These regulators accompany with identified drought-related genes could be valuable candidates for future studies and breeding programs on Sesame under drought stress. Keywords: Sesamum indicum, Drought stress, Regulatory networks, miRNA, Transcription Factors.

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Functional analysis of a grape WRKY30 gene in drought resistance

Cited by 23 publications

References 32 publications

Meta-analysis of the effects of overexpression of WRKY transcription factors on plant responses to drought stress

Meta-analysis of the effects of overexpression of WRKY transcription factors on plant responses to drought stress

VvBAP1 Is Involved in Cold Tolerance in Vitis vinifera L.

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

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