Functional analysis and tissue-differential expression of four FAD2 genes in amphidiploid Brassica napus derived from Brassica rapa and Brassica oleracea

Lee, Kyeong‐Ryeol; Sohn, Soo-In; Jung, Jin Hee; Kim, Sun Hee; Roh, Kyung Hee; Kim, Jong Bum; Suh, Mi Chung; Kim, Hyun Uk

doi:10.1016/j.gene.2013.08.095

Cited by 38 publications

(47 citation statements)

References 52 publications

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“…(Table 1), we found that seven AsFAD2 proteins were located in the ER membrane ( Fig. 6), which was in agreement with previous findings for cotton FAD2-4:GFP [ 30], three BnFAD2s:YFP [ 13] and PfrFAD2-1:GFP [ 51]. These results further confirmed that the core reaction of LA biosynthesis in plants occurs in the ER and suggested that other AsFAD2 enzymes may be ER-localized.…”

Section: Discussionsupporting

confidence: 91%

“…With the exception of Arabidopsis thaliana, which has only one FAD2 gene [ 11], while most plants have multiple FAD2 genes. Thus, olive (Olea europaea) has two FAD2 genes [ 12], Oilseed rape (Brassica napus) has four [ 13], peanut (Arachis hypogaea) has six [ 14], safflower (Carthamus tinctorius) has eleven [ 15], and Artemisia sphaerocephala has twenty-six [ 16]. Gene families usually occur through gene duplication and mutations, and the variations in the number of gene family members is an important evolutionary mechanism underlying functional diversity and shaping genomic adaptation in various species [ 17].…”

Section: Introductionmentioning

confidence: 99%

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Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Miao

zhang

et al. 2019

Preprint

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Background: Linoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Δ12) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis. Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The objective of this work is to study the difference and potentially functionality of AsFAD2 family members. Results: Full-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable, suggesting that the AsFAD2 family may play an important role in the adaptation of A. sphaerocephala to the harsh desert environment. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, -10, and -23, were Δ12 oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2-1 and AsFAD2-10 could also produce palmitolinoleic acid. Conclusions: This research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding of fatty acid biosynthesis in A. sphaerocephala, and can be applied in the study of plant fatty acids traits.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Miao

zhang

et al. 2019

Preprint

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“…Well-studied genes such as FAE1, TAG1, and FAD2 in Arabidopsis, B. napus, and other oilseed crops were reported as candidate genes for the synthesis of linoleic acid, linolenic acid, erucic acid, oleic acid, or total oil content (Peng et al, 2010;Yang et al, 2012;Lee et al, 2013). Cis-eQTL of BrFAD2 and other BrFAD genes (BrFAD5 and BrFAD7) and trans-eQTL of BrFAE1 and BrTAG1 genes colocalized with faQTLs for oleic acid (C18:2), linoleic acid (C18:2), erucic acid (C22:1), and other FAs ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…At the major eQTL hotspot on A09 (89 trans-eQTLs), where the cis-regulated BrTT8 gene for seed color is located, we did not detect major faQTLs. We looked for colocation of eQTLs of the genes BrFAE1, TAG1 (also called DGAT1), BrFAD2, and other BrFAD genes with faQTLs, as these genes were reported as candidate genes for the synthesis of linoleic acid, linolenic acid, erucic acid, oleic acid, or total oil content in Arabidopsis and B. napus (Peng et al, 2010;Yang et al, 2012;Lee et al, 2013). BrFAE1 has two paralogs on A01 and A03, which only have trans-eQTLs on A02, A03, A05, and A07, and BrTAG1 on A07 has a transeQTL on A05 (Supplemental Fig.…”

Section: Colocalization Of Metabolite Qtls and Eqtlsmentioning

confidence: 99%

“…Across a number of studies, a large number of QTLs for FAs and oil content have been reported, suggesting a complex genetic architecture (Yan et al, 2011;Sanyal and Randal Linder, 2012). Genetic studies have identified the FA desaturase genes BnaFAD2 and BnaFAD3 as the major genes for the regulation of C18:1 (oleic acid) and C18:3 (linolenic acid) content in B. napus (Peng et al, 2010;Yang et al, 2012;Lee et al, 2013). BnaFAE1 is a candidate gene for erucic acid and total oil content in B. napus seed (Peng et al, 2010), while BrFAD3 is a candidate gene for the synthesis of linolenic acid in seed triacylglycerols in B. rapa ssp.…”

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confidence: 99%

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A Systems Genetics Approach Identifies Gene Regulatory Networks Associated with Fatty Acid Composition in Brassica rapa Seed

et al. 2015

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Fatty acids in seeds affect seed germination and seedling vigor, and fatty acid composition determines the quality of seed oil. In this study, quantitative trait locus (QTL) mapping of fatty acid and transcript abundance was integrated with gene network analysis to unravel the genetic regulation of seed fatty acid composition in a Brassica rapa doubled haploid population from a cross between a yellow sarson oil type and a black-seeded pak choi. The distribution of major QTLs for fatty acids showed a relationship with the fatty acid types: linkage group A03 for monounsaturated fatty acids, A04 for saturated fatty acids, and A05 for polyunsaturated fatty acids. Using a genetical genomics approach, expression quantitative trait locus (eQTL) hotspots were found at major fatty acid QTLs on linkage groups A03, A04, A05, and A09. An eQTL-guided gene coexpression network of lipid metabolism-related genes showed major hubs at the genes BrPLA2-ALPHA, BrWD-40, a number of seed storage protein genes, and the transcription factor BrMD-2, suggesting essential roles for these genes in lipid metabolism. Three subnetworks were extracted for the economically important and most abundant fatty acids erucic, oleic, linoleic, and linolenic acids. Network analysis, combined with comparison of the genome positions of cis-or trans-eQTLs with fatty acid QTLs, allowed the identification of candidate genes for genetic regulation of these fatty acids. The generated insights in the genetic architecture of fatty acid composition and the underlying complex gene regulatory networks in B. rapa seeds are discussed.

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Genetic Improvement of Oil Quality Using Molecular Techniques in Brassica juncea

Yashpal

Saini

Singh

et al. 2020

Brassica Improvement

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Functional analysis and tissue-differential expression of four FAD2 genes in amphidiploid Brassica napus derived from Brassica rapa and Brassica oleracea

Cited by 38 publications

References 52 publications

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

A Systems Genetics Approach Identifies Gene Regulatory Networks Associated with Fatty Acid Composition in Brassica rapa Seed

Genetic Improvement of Oil Quality Using Molecular Techniques in Brassica juncea

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