Functional analysis and application of the cryptic plasmid pBSG3 harboring the RapQ–PhrQ system in Bacillus amyloliquefaciens B3

Qiao, Jun; Tian, Dacheng; Huo, Rong; Wu, Hui; Gao, Xue

doi:10.1016/j.plasmid.2010.11.008

Cited by 14 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A plasmid was evident from strain AP143. High coverage contigs from the assembled genome sequence of AP143 (data not shown) were screened by BLASTn and one of the contigs showed high identity (99%) with plasmid pBSG3 from Bacillus amyloliquefaciens B3 [31] . BLASTx analysis of the predicted open reading frames from this plasmid did not show any similarity to genetic loci involved in antibiotic resistance or virulence.…”

Section: Resultsmentioning

confidence: 99%

Identification of Bacillus Strains for Biological Control of Catfish Pathogens

et al. 2012

View full text Add to dashboard Cite

Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Yersinia ruckeri, Flavobacterium columnare, and/or the oomycete Saprolegnia ferax. Survival of the 21 Bacillus strains in the intestine of catfish was determined as Bacillus CFU/g of intestinal tissue of catfish after feeding Bacillus spore-supplemented feed for seven days followed by normal feed for three days. Five Bacillus strains that showed good antimicrobial activity and intestinal survival were incorporated into feed in spore form at a dose of 8×107 CFU/g and fed to channel catfish for 14 days before they were challenged by E. ictaluri in replicate. Two Bacillus subtilis strains conferred significant benefit in reducing catfish mortality (P<0.05). A similar challenge experiment conducted in Vietnam with four of the five Bacillus strains also showed protective effects against E. ictaluri in striped catfish. Safety of the four strains exhibiting the strongest biological control in vivo was also investigated in terms of whether the strains contain plasmids or express resistance to clinically important antibiotics. The Bacillus strains identified from this study have good potential to mediate disease control as probiotic feed additives for catfish aquaculture.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification of Bacillus Strains for Biological Control of Catfish Pathogens

et al. 2012

View full text Add to dashboard Cite

show abstract

“…8 ). Studies have shown that some species of the Bacillus genus produce antibiotics and fungal cell wall degrading enzymes such as chitinase and glucoamylase; while some species carry genes related to indole-3-acetic acid production and ferredoxin improving plant growth 55 – 58 . In addition, according to the analysis of bacterial functional analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

Liu

Wang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The soil-borne disease caused by Fusarium graminearum seriously affects the corn quality. Straw can greatly improve soil quality, but the effect is limited by its nature and environmental factors. This study explored the impact of straw-JF-1(biocontrol bacteria) combination on soil environment and soil disease resistance. The results showed that the combined treatment increased the proportion of soil large and small macro-aggregates by 22.50 and 3.84%, with soil organic carbon (SOC) content by 16.18 and 16.95%, respectively. Compared to treatment with returning straw to the field alone, the straw-JF-1 combination increased the soil content of humic acid, fulvic acid, and humin by 14.06, 5.50, and 4.37%, respectively. Moreover, A metagenomics showed that returning straw to the field alone increased the abundance of disease-causing fungi (Fusarium and Plectosphaerella), however, the straw-JF-1 combination significantly suppressed this phenomenon as well as improved the abundance of probiotic microorganisms such as Sphingomonas, Mortierella, Bacillus, and Pseudomonas. Functional analysis indicated that the combination of straw and JF-1 improved some bacterial functions, including inorganic ion transport and metabolism, post-translational modification/protein turnover/chaperones and function unknown, fungal functions associated with plant and animal pathogens were effectively inhibited. Pot experiments showed that the straw-JF-1 combination effectively inhibited the Fusarium graminearum induced damage to maize seedlings. Therefore, the combination of straw and JF-1 could be a practical method for soil management.

show abstract

“…Additionally, peptides such as nisin and subtilin, encoded by nisB, nisC, and spaS, are bacteriocin effective against many Gram-positive organisms, functioning as probable probiotics with antimicrobial activities [67][68][69]. Moreover, B. subtilis SRCM103571 and SRCM103689 specifically possess genes encoding response regulators of aspartate phosphatase I and SaeR, which are involved in sporulation and adhesion to the host cells [70,71]. recombination, and repair; D, Cell cycle control, cell division, and chromosome partitioning; V, Defense mechanisms; T, Signal transduction mechanisms; M, Cell wall/membrane/envelope biogenesis; N, Cell motility; O, Posttranslational modification, protein turnover, and chaperones; C, Energy production and conversion; G, Carbohydrate transport and metabolism; E, Amino acid transport and metabolism; F, Nucleotide transport and metabolism; H, Coenzyme transport and metabolism; I, Lipid transport and metabolism; P, Inorganic ion transport and metabolism; Q, Secondary metabolite biosynthesis, transport, and catabolism.…”

Section: Comxmentioning

confidence: 99%

Comparative Genomic and Functional Evaluations of Bacillus subtilis Newly Isolated from Korean Traditional Fermented Foods

Choi

Shin

et al. 2020

Foods

View full text Add to dashboard Cite

Many fermented foods are known to have beneficial effects on human and animal health, offering anti-aging and immunomodulatory benefits to host. Microorganisms contained in the fermented foods are known to provide metabolic products possibly improving host health. However, despite of a number of studies on the functional effects of the fermented foods, isolation and identification of the effective bacterial strains in the products are still in progress. The objective of this study was to isolate candidate functional strains in various Korean traditional fermented foods, including ganjang, gochujang, doenjang, and jeotgal, and evaluate their beneficial effects on the host, using Caenorhabditis elegans as a surrogate animal model. Among the 30 strains isolated, five Bacillus spp. were selected that increased the expression level of pmk-1, an innate immune gene of C. elegans. These strains extended the nematode lifespan and showed intestinal adhesion to the host. Based on the bioinformatic analyses of whole genome sequences and pangenomes, the five strains of Bacillus subtilis were genetically different from the strains found in East Asian countries and previously reported strains isolated from Korean fermented foods. Our findings suggest that the newly isolated B. subtilis strains can be a good candidate for probiotic with further in-depth investigation on health benefits and safety.

show abstract

Functional analysis and application of the cryptic plasmid pBSG3 harboring the RapQ–PhrQ system in Bacillus amyloliquefaciens B3

Cited by 14 publications

References 34 publications

Identification of Bacillus Strains for Biological Control of Catfish Pathogens

Identification of Bacillus Strains for Biological Control of Catfish Pathogens

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

Comparative Genomic and Functional Evaluations of Bacillus subtilis Newly Isolated from Korean Traditional Fermented Foods

Contact Info

Product

Resources

About