Functional Activity of the Photosynthetic Apparatus in Tobacco and Arabidopsis Plants Exposed to Chilling Temperatures

Popov, V. N.; Antipina, O. V.; Selivanov, A. A.; Рахманкулова, З. Ф.; Deryabin, A. N.

doi:10.1134/s1021443719010138

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…Their functional role is associated with the processes of absorption of light energy, as well as participation in the protection of cells from stress [60,61]. An increase in carotenoid content is observed when tobacco plants are exposed to low temperatures [59]. In our work, the same stress response occurred in WT and FeSOD-transgenic tobacco plants, based on the accumulation of carotenoids in them as one of the components of cell protection from stress [61].…”

Section: Discussionsupporting

confidence: 65%

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Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage

Baranova,

Kononenko,

Lapshin

et al. 2024

IJMS

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ROS-dependent induction of oxidative damage can be used as a trigger initiating genetically determined non-specific protection in plant cells and tissues. Plants are potentially able to withstand various specific (toxic, osmotic) factors of abiotic effects, but do not have sufficient or specific sensitivity to form an adequate effective response. In this work, we demonstrate one of the possible approaches for successful cold acclimation through the formation of effective protection of photosynthetic structures due to the insertion of the heterologous FeSOD gene into the tobacco genome under the control of the constitutive promoter and equipped with a signal sequence targeting the protein to plastid. The increased enzymatic activity of superoxide dismutase in the plastid compartment of transgenic tobacco plants enables them to tolerate the oxidative factor of environmental stresses scavenging ROS. On the other hand, the cost of such resistance is quite high and, when grown under normal conditions, disturbs the arrangement of the intrachloroplastic subdomains leading to the modification of stromal thylakoids, probably significantly affecting the photosynthesis processes that regulate the efficiency of photosystem II. This is partially compensated for by the fact that, at the same time, under normal conditions, the production of peroxide induces the activation of ROS detoxification enzymes. However, a violation of a number of processes, such as the metabolism of accumulation, and utilization and transportation of sugars and starch, is significantly altered, which leads to a shift in metabolic chains. The expected step for further improvement of the applied technology could be both the use of inducible promoters in the expression cassette, and the addition of other genes encoding for hydrogen peroxide-scavenging enzymes in the genetic construct that are downstream in the metabolic chain.

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Section: Discussionsupporting

confidence: 65%

“…A similar result was observed in Arabidopsis thaliana Heynh. (L.) after exposure to low positive temperatures, where no significant changes in the content of photosynthetic pigments were also detected [59].…”

Section: Discussionmentioning

confidence: 98%

Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage

Baranova,

Kononenko,

Lapshin

et al. 2024

IJMS

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“…Our result showed that upwelling treatment induced qN and enhanced the photoprotective mechanism. The Fv/Fm of all the seedlings obtained in this study were close to 0.8, showing that impairment in plant health status did not occur [57]. The values of Y(II), ETR, and qP values showed a decrease compared to the control but no significant change.…”

Section: Response Of the Photosynthetic System Of K Obovata Seedlings...supporting

confidence: 57%

Physiological and Biochemical Responses of Kandelia obovata to Upwelling Stress

Wang

Dong

et al. 2022

Water

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Mangroves growing in intertidal areas are faced with various stresses caused by coastal human activities and oceanic and atmospheric sources. Although the study of the physiological and biochemical characteristics of mangroves has been developing over the past four decades, the effect of upwelling on mangroves in plants stress resistance has seldom been investigated. Here, changes in the physiological and biochemical characteristics of the leaves of Kandelia obovata seedlings in response to upwelling were investigated (air temperature: 25 °C; water temperature: control 25 °C, 13 °C, and 5 °C; salinity: 10‰). The results revealed that upwelling treatment caused an increase in chlorophyll content but a decrease in photosynthetic fluorescence parameters. Hydrogen peroxide (H2O2) production and malondialdehyde activity (MDA) increased with the decrease in upwelling temperature. The proline content increased under upwelling stress, whereas the soluble sugar content decreased. Further, the activities of antioxidant enzymes, such as superoxide dismutase activity (SOD) and peroxidase activity (POD), showed an increasing trend during the treatment, while catalase activity (CAT) decreased. It was evidenced that upwelling stress triggered the physiological and biochemical responses of Kandelia obovata seedlings. This effect became more intense as the upwelling temperature decreased, and all these indicators showed different responses to upwelling stress. Through synthesizing more energy and regulating enzyme activity and osmotic pressure, the leaves of K. obovata formed a resistance mechanism to short-term upwelling.

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“…In accordance with our results, Renaut et al 6 reported the highest content of glucose, fructose and sucrose in poplar leaves after 2 days of chilling (4 °C) compared to control. Popov et al 42 also reported that the total sugar content in tobacco and Arabidopsis leaves increased by 30 and 60%, respectively, on the sixth day of low temperature exposure (8 and 2 °C), mainly due to the increase in sucrose.…”

Section: Discussionmentioning

confidence: 94%

Response of chloroplast pigments, sugars and phenolics of sweet cherry leaves to chilling

Vošnjak

Šircelj

Hudina

et al. 2021

Sci Rep

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The aim of the present study was to evaluate the effect of post-flowering chilling of sweet cherry (Prunus avium L.) on the content of biochemical parameters in the leaf (chloroplast pigments, sugars and phenolics). The effect of chilling was investigated in two experiments. Potted 2-year-old trees of cv. 'Grace Star' and 'Schneiders' were exposed to one, two or three consecutive overnight chillings at an average air temperature of 4.7 °C (Experiment I), but in the following year only trees of 'Grace Star' were chilled at 2.2 °C (Experiment II), 3 to 7 weeks after flowering. The analysis of the biochemical parameters was performed by high performance liquid chromatography combined with electrospray ionization mass spectrometry. Chilling at 4.7 °C caused little or no stress, while 2.2 °C induced more intense stress with increased zeaxanthin, sugar and phenolic content in leaves, while exposure of trees to higher temperatures and closer to flowering showed no changes. Two or three consecutive overnight chilling periods increased the phenolic content and enhanced the accumulation of zeaxanthin in the leaves. Sucrose, sorbitol, fructose, total sugar, and total flavonoid content in leaves increased within 48 h after chilling. Zeaxanthin epoxidized within 24 h after one and 48 h after one and two consecutive overnight chillings.

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Functional Activity of the Photosynthetic Apparatus in Tobacco and Arabidopsis Plants Exposed to Chilling Temperatures

Cited by 5 publications

References 25 publications

Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage

Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage

Physiological and Biochemical Responses of Kandelia obovata to Upwelling Stress

Response of chloroplast pigments, sugars and phenolics of sweet cherry leaves to chilling

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