Functional Activity of Non-Proliferating Mesenchymal Stromal Cells Cultured at Different Densities

Ездакова, М. И.; Zornikova, Ksenia V.; Buravkov, S. V.; Андреева, Е.Р.

doi:10.1007/s10517-021-05102-w

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…Insufficient blood volume may lead to a lack of cell numbers, which can affect the preparation and examination of chromosomes. In cell culture, cell density is a critical factor influencing cell proliferation and division [22,23]. For instance, pre-B acute lymphoblastic leukemia cells (ALL3) exhibit poor or no growth under low-density conditions, yet thrive better under high initial cell density conditions.…”

Section: Discussionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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Objective: This study aims to investigate the impact of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis using response surface methodology (RSM). Methods: Peripheral blood samples from 30 volunteers were subjected to chromosomal analysis under different refrigeration duration periods (≤7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days) along with different blood volumes (0.2 mL, 0.3 mL, 0.4 mL, 0.5 mL, 0.6 mL, 0.7 mL, and 0.8 mL). The effects of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis were determined using the Chi-square test for trend, followed with Spearman's rank correlation coefficient, and RSM analysis to identify the optimal combination of refrigeration time and blood volume. Results: The refrigeration time within 10 days had a minor impact on the success rate, while refrigeration time more than 11 days significantly decreased the success rate. An increase in blood volume slightly improved the success rate. The success rate showed both linear and nonlinear changes with refrigeration time, while the effect of blood volume was primarily linear. The highest success rate was observed at a refrigeration time of ≤7 days and a blood volume of 0.8 mL. The interaction between refrigeration time and blood volume had a significant impact on the success rate. Conclusion: It is recommended to keep the refrigeration time of blood samples within 7 days and control the blood volume at 0.8 mL to maximize the success rate of chromosomal analysis.

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Section: Discussionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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The Sensitivity of Multipotent Mesenchymal Stromal Cells to Short-Term Hypoxic Stress In Vitro Depends on the Efficiency of Homotypic Communication through Gap Junctions

Ezdakova,

Matveeva,

Andrianova

et al. 2023

Moscow Univ. Biol.Sci. Bull.

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The sensitivity of multipotent mesenchymal stromal cells to short-term hypoxic stress in vitro depends on the efficiency of homotypic communication through gap junctions

Ezdakova,

Matveeva,

Andrianova

et al. 2023

Vestn. Mosk. Univ. Ser. 16. Biol.

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Gap junctions (GJ) provide metabolic cooperation between cells through the direct exchange of cytoplasmic components. We analyzed the effect of short-term hypoxic stress on the efficiency of communication through the GJs in cultured multipotent mesenchymal stromal cells (MSCs) and characterized the sensitivity of MSCs to short-term hypoxic stress depending on the GJ function. Mitotically inactive MSCs were used in the experiments, in which the GJs were blocked with a specific inhibitor – carbenoxolone. The MSCs were continuously cultured at 20% O2. Further, MSCs with blocked and working GJs were subjected to hypoxic stress (0.1%, 24 hours). The efficiency of GJ communication was attenuated under hypoxic stress. The combined action of GJ inhibition and hypoxic stress was accompanied by an increase in ROS level as compared to the MSCs after hypoxic stress only. MSCs with blocked GJs were less sensitive to short-term hypoxic stress in comparison with MSCs integrated into the common network through working GJs. It was manifested in attenuation of hypoxia-induced angiogenic activity of MSCs. The angiogenic effects of conditioned medium from the MSCs with blocked GJs were almost twice less, which seems to be related to differences in the angiogenic mediators’ profiles: VEGF level decreased and FGF-2 level increased, while the monocyte chemoattractant protein 3 (MCP-3) level was unchanged. Thus, a decrease in the efficiency of direct MSCs- MSCs communication had a negative effect on mostly requested MSCs activity – the ability to induce angiogenesis. We conclude that blocking of GJ communication in MSCs is a negative event that impairs the coordination of MSCs’ response to the microenvironmental factors, in particular hypoxic stress, and reduces their functional plasticity.

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Functional Activity of Non-Proliferating Mesenchymal Stromal Cells Cultured at Different Densities

Cited by 3 publications

References 26 publications

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

The Sensitivity of Multipotent Mesenchymal Stromal Cells to Short-Term Hypoxic Stress In Vitro Depends on the Efficiency of Homotypic Communication through Gap Junctions

The sensitivity of multipotent mesenchymal stromal cells to short-term hypoxic stress in vitro depends on the efficiency of homotypic communication through gap junctions

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