Functional activity of neutrophils and local manifestations of oxidative stress in the mucous membrane of the nasal cavity in the early postoperative period

Гизингер, О. А.; Коркмазов, А. М.; Сумеркина, В. А.

doi:10.17116/otorino20198402140

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Биохимические исследования включали определение изопропанол-и гептанрастворимых первичных, вторичных, конечных продуктов перекисного окисления липидов. Активность антиоксидантной защиты изучена по уровню супероксиддисмутазы и каталазы по методу С. Чевари («Технология-Стандарт», Россия) [5].…”

Section: характеристика детей и методы исследованияunclassified

“…При остром риносинусте выявлено повышение концентрации продуктов перекисного окисления липидов, выраженное в достоверном увеличении концентрации первичных, вторичных и третичных продуктов перекисного окисления по сравнению с их содержанием в назальном секрете в группе контроля, что прямо свидетельствует о наличии оксидативных изменений на поверхности слизистых оболочек. Механизм формирования таких нарушений проявляется рассогласованием реакций антиоксидантной защиты, истощением эндогенных антиоксидантов, активизацией свободнорадикальных реакций, следствием чего является повышение уровня перекисного окисления липидов, приводящее к деструкции биологических мембран [5].…”

Section: результаты и обсуждениеunclassified

“…У таких детей встречаются вторичные осложнения, снижение социального, адаптационного потенциала [4]. Спектр выявляемых при риносинуситах дисфункций затрагивает важные звенья гомеостатического регулирования: нарушение количественного состава и потенциала нейтрофильных гранулоцитов, формирование окислительного стресса на поверхности слизистых оболочек путем нарушения функ-РОССИЙСКИЙ ВЕСТНИК ПЕРИНАТОЛОГИИ И ПЕДИАТРИИ, 2020; 65: (2) ROSSIYSKIY VESTNIK PERINATOLOGII I PEDIATRII, 2020; 65: (2) В ПОМОЩЬ ПРАКТИЧЕСКОМУ ВРАЧУ ционирования ключевых медиаторов про/антиоксидантной системы [5]. Солевые растворы морской воды гипертонической концентрации входят в большинство схем лечения заболеваний слизистой оболочки полости носа и околоносовых пазух, разработанных медицинскими сообществами EPOS, ARIA, European Position Paperon Rhinosinusitis and Nasal Polyps [6,7].…”

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Hypertonic seawater solution with brown algae extract Ascophillum nodosum in the treatment of acute rhinosinusitis

Гизингер

2020

Ross. vestn. perinatol. pediatr.

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The article presents modern approaches to the modulation of cellular factors of innate immunity, oxidative stress factors when using a hypertonic seawater solution with Ascophillum nodosum brown algae extract (NaCl 15 g/l) in patients with acute rhinosinusitis.Characteristics of the children and research methods. The authors examined 102 children aged from 5 to 8 years, with a history of more than 5 episodes of acute rhinosinusitis per year. We evaluated the quantitative and qualitative composition of neutrophilic granulocytes in washes from the mucous membrane of the nasal cavity, the production of reactive oxygen species (ROS) by neutrophilic granulocytes, the content of lipid peroxidation products, enzymes of the antioxidant system – superoxide dismutase and catalase in nasal washes.Results. The authors found that the nasal spray of a hypertonic seawater solution with Ascophillum nodosum brown algae extract helps to normalize clinical picture, reduce number of relapses, restore functional and metabolic status of neutrophils of the nasal mucosa surface, restore balance of lipid peroxidation factors, antioxidant sustem enzymes – superoxide dismutase and catalase.

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Section: характеристика детей и методы исследованияunclassified

Section: результаты и обсуждениеunclassified

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Hypertonic seawater solution with brown algae extract Ascophillum nodosum in the treatment of acute rhinosinusitis

Гизингер

2020

Ross. vestn. perinatol. pediatr.

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A clinical and immunological rationale for ultrasonic cavitation use in the complex treatment of infertility in chronic endometritis

2022

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Aim.To prove the safety and efficacy of using the ultrasonic cavitation of saline to restore the endometrial receptivity, local immune balance, and antioxidant status in females with chronic endometritis (CE) and a history of reproductive losses. Materials and methods. Fifty females aged 29.957.65 years (range 2338 years) with histologically and immunohistochemically confirmed CE were examined. The control group included 45 female patients aged 28.989.9 years (range 2338 years) with infertility of tubal-peritoneal genesis without histochemical studies confirming CE and with no history of reproductive losses. Transvaginal pelvic ultrasound was used in the study. All the patients had histological, immunohistochemical examination of endometrial scrapings, including quantification of CD138+, CD20+, CD56+, progesterone receptors (PR), and estrogen receptors (ER) in the stroma and glands. The following cytokines were measured in protein-free fractions of uterine cavity contents by ELISA: interleukin (IL)-2, IL-10, IL-8, tumor necrosis factor-, IL-1, interferon-, interferon-. The activity of lipoperoxidation factors and antioxidant system enzymes (catalase, superoxide dismutase, glutathione peroxidase) was detected by spectrophotometry. As a part of the treatment, irrigation of the uterine cavity with sonicated 0.9% sodium chloride solution was applied twice in 6 sessions consecutively within three months. Results. According to transvaginal pelvic ultrasound, the endometrial thickness before treatment was 6.541.2 mm. An increase in endometrial thickness up to 9.02 (7.6210.42) mm in the luteal phase of the next cycle and up to 9.34 (8.1210.56) mm in the two subsequent luteal phases was observed within three months after irrigation of the uterine cavity with sonicated normal saline. The normalization of the ЕR/PR ratio and decrease in CD138+, CD20+, and CD56+ cells in the endometrial stroma and glands were noted. Mature pinopods were revealed in 71.25 (67.2974.46)% of patients, which is 2.95 times more than the respect value before the treatment, 24.55 (21.4327.75)%. After treatment, a recovery of cytokine production was noted: IL-2, IL-10, tumor necrosis factor-, IL-8, IL-1, interferon-, interferon-; concentration of primary, secondary and tertiary products of lipoperoxidation decreased; superoxide dismutase enzyme activity increased by 36.98 (32.8841.18)%, catalase by 15.32 (14.1316.51)%, glutathione peroxidase by 12.32 (11.1214.2)%; р0,005. Reduced colonization rate of Streptococcus spp, Enterococcus faecalis, Escherichia coli, elimination of Mycoplasma genitalium, Ureaplasma urealyticum, and increased of Lactobacillus spp. were reported. Conclusion. Using the cavitated normal saline restores the endometrium structure, normalizes the ЕR/PR ratio, reduces the colonization by opportunistic and pathogenic microorganisms in the uterine cavity and normalizes the cytokine balance and the content of pro-oxidative and anti-oxidative factors. High clinical and immunological efficacy supports cavitated solutions in the complex treatment of infertility in females with CE.

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The role of antimicrobial defense factors in the pathogenesis of immune disorders in deviated septum. Part 2

Kornova

2021

Terapevt (General Physician)

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The article presents the data from Russian and foreign researchers on the state of humoral and cellular factors of antimicrobial protection of the mucous membrane of the nasal cavity and paranasal sinuses in the pathogenesis of immune disorders in deviated septum. One of the factors affecting the intensity of the inflammatory reaction and the rate of epithelialization of the mucous membrane is a decrease in IL-10 receptor expression, and, as a consequence, a decrease in its endogenous production. In vivo experiments have shown that the reduced expression of genes responsible for the synthesis of interleukin-10 inhibits infiltration of the wound postoperative surface of phagocytes, reducing the expression of CC-chemokines (MCP-1, MIP-1α), their receptors, cytokines, and interleukin-1α. The role of cellular and humoral factors, interreceptor interactions of cells of the respiratory, glandular epithelium, phagocytes, and lymphocytes in pathological conditions of the nasal mucosa and paranasal sinuses was analyzed. The role of immune mechanisms in the development of pathological reactions in deviated septum is shown. The presented review will expand the understanding of the role of antimicrobial factors in the pathogenesis of immune disorders in deviated septum.

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Functional activity of neutrophils and local manifestations of oxidative stress in the mucous membrane of the nasal cavity in the early postoperative period

Cited by 4 publications

References 6 publications

Hypertonic seawater solution with brown algae extract Ascophillum nodosum in the treatment of acute rhinosinusitis

Hypertonic seawater solution with brown algae extract Ascophillum nodosum in the treatment of acute rhinosinusitis

A clinical and immunological rationale for ultrasonic cavitation use in the complex treatment of infertility in chronic endometritis

The role of antimicrobial defense factors in the pathogenesis of immune disorders in deviated septum. Part 2

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