Function of Transferrin

Fletcher, J.; Huehns, E. R.

doi:10.1038/2181211a0

Cited by 219 publications

(84 citation statements)

References 26 publications

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“…The recent observation of Leibman and Aisen (50) that iron on monoferric transferrin circulating in the plasma of normal human subjects has a selective orientation on one of the two iron binding sites of transferrin raises the possibility of selective placental binding of transferrin in pregnancy (49). Since under our experimental conditions no metabolic conversion or scrambling of iron on transferrin seems possible with the microvillous membrane preparation, it appears ideal for the study of the biological implications of the FletcherHuehns hypothesis.…”

Section: Discussionmentioning

confidence: 96%

Transferrin receptors on the human placental microvillous membrane.

Loh¹,

Higuchi²,

Bockxmeer³

et al. 1980

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 96%

Transferrin receptors on the human placental microvillous membrane.

Loh¹,

Higuchi²,

Bockxmeer³

et al. 1980

J. Clin. Invest.

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“…Measurement of the ratio 59Fe:55Fe in tagged tissues of normal and iron-deficient rats (Table V) (21)(22)(23)(24)(25), and alternate possibilities have been suggested. One is that the protein is able to pass iron selectively from its individual sites to site-specific tissues and by differential loading and unloading to direct the flow ofiron (26). The other possibility is that transferrin acts as a general transport vehicle and that the exchange of iron is dictated by the amount of iron entering the circulation and the amount removed by individual tissues according to the number of receptors for iron which they possess.…”

Section: Resultsmentioning

confidence: 99%

“…Next, ferrous ammonium sulfate (750 j,g Fe dissolved in 2 ml 0.01 N HCI) was mixed with 75 ,uCi 59Fe (as 59FeSO4, sp act 6-20 ,uCi/,Lg, dissolved in 0.5 M HC1, New England Nuclear, Boston, Mass.) or with 225 ,Ci 55Fe (as 55FeSO4, sp act [20][21][22][23][24][25][26][27][28][29][30] ,uCi/,g, dissolved in 0.5 M HCI) from the same manufacturer. The added iron was sufficient to exceed the transferrin saturation by about 20%, and the final pH was 8.1.…”

Section: Methodsmentioning

confidence: 99%

Iron uptake from rat plasma transferrin by rat reticulocytes.

Huebers¹,

Huebers²,

Csiba³

et al. 1978

J. Clin. Invest.

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A B S T R A C T Fast and slow rat transferrins were isolated by isoelectric focusing and prepared in their di-and monoferric forms. A comparison of the rates of iron release between fast and slow diferric transferrins and between fast and slow monoferric transferrins when incubated with reticulocytes or injected in vivo showed no significant difference in the behavior of the two isotransferrin species. Reticulocyte uptake of diferric transferrin resulted in the removal of both iron atoms from the transferrin molecule. A twofold greater iron uptake was observed from diferric as compared with monoferric iron, provided reticulocyte receptors were saturated. It is concluded that the two species of transferrin and their individual sites function similarly in their release of iron to tissue receptors.

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“…Transferrin is a l,8 globulin with a molecular weight of 86,000 and is the major iron transport protein in the blood (18). Transferrin is required to sustain cell division in 3T6, V79, and hemopoietic cells (19)(20)(21).…”

Section: Discussionmentioning

confidence: 99%

Growth of a rat neuroblastoma cell line in serum-free supplemented medium.

Bottenstein¹,

Sato²

1979

Proc. Natl. Acad. Sci. U.S.A.

1,945

983

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The rat neuroblastoma B104 cell line, which originated in the central nervous system, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine. When added individually, each supplement had little or no effect; however, in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium, and other differentiated properties were retained as well. Synthetic media generally require serum supplementation to support the proliferation or survival of cultured cells. The inclusion of serum, however, may significantly affect experimental reproducibility, because batch variations occur as a result of differences in donor age, sex, nutrition, and physiological state even in pooled serum samples. In addition, the complex undefined nature of serum is a complication when assessing the effect(s) of regulatory agents, such as hormones or neurotransmitters, on differentiated properties of nervous system cells in culture. This is particularly important for long-term studies, because if serum is deleted a substantial reduction of cell numbers, and in many cases complete cell death, may occur within hours or a few days.In order to circumvent these problems, several cell lines have been adapted to proliferate in serum-free media (1-4). How The B104 rat neuroblastoma, a cell line of central nervous system origin, exhibits many of the properties characteristic of differentiated neurons, such as generation of action potentials, synthesis of neurotransmitters, and presence of neurotransmitter receptors and the neuron-specific 14-3-2 protein (9, 10). Furthermore, B104 cells, like C1300 neuroblastoma cells (11, 12), respond to removal of serum by rapidly extending neurites, a phenomenon that has been correlated with the preceding neuronal properties (9).In this communication we report that B104 cells can proliferate in a serum-free synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine (N2 medium). More extensive process formation is seen in N2 than in serum-supplemented medium, and other differentiated properties have been retained as well. METHODS AND MATERIALSCell Culture. The rat neuroblastoma B104 cell line was obtained from D. Schubert of the Salk Institute, La Jolla, CA. Stock cultures were maintained in a 1:1 mixture of Ham's F12 medium and the Dulbecco-Vogt modification of Eagle's medium (DME) supplemented with 5% (vol/vol) horse serum; 2.5% (vol/vol) fetal calf serum; 1.2 g of NaHCO3 per liter; 15 mM Hepes buffer; and 40 mg of penicillin, 8 mg of ampicillin, and 90 mg of streptomycin per liter. Triple-distilled water was used to prepare media. Stock cultures were grown in flasks (Falcon Plastics 3013, 25-cm2 surface area) in 4 ml of medium in a humidified atmosphere of 5% C02/95% air at 370C and subcultured every ...

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Function of Transferrin

Cited by 219 publications

References 26 publications

Transferrin receptors on the human placental microvillous membrane.

Transferrin receptors on the human placental microvillous membrane.

Iron uptake from rat plasma transferrin by rat reticulocytes.

Growth of a rat neuroblastoma cell line in serum-free supplemented medium.

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