Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea

An, Bang; Li, Boqiang; Qin, Guozheng; Tian, Shiping

doi:10.1016/j.fgb.2015.01.007

Cited by 35 publications

(26 citation statements)

References 45 publications

(59 reference statements)

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“…This suggests that this collection of B. cinerea genotypes does not contain large-effect qualitative virulence loci; instead, this generalist uses a highly polygenic virulence architecture (Denby et al, 2004;Finkers et al, 2007b;Rowe and Kliebenstein, 2008;Corwin et al, 2016b;Zhang et al, 2016). This agrees with previous B. cinerea mechanistic studies that identify a large collection of standing genetic variation that controls a wide range of specific virulence mechanisms (Choquer et al, 2007;Rowe and Kliebenstein, 2007;Noda et al, 2010;Rowe et al, 2010;Dalmais et al, 2011;Michielse et al, 2011;Shlezinger et al, 2011;Windram et al, 2012;Pearson and Bailey, 2013;Kumari et al, 2014;An et al, 2015;Atwell et al, 2015;Hevia et al, 2015;Plaza et al, 2015;Schumacher et al, 2015;Zhang et al, 2015a;Corwin et al, 2016aCorwin et al, , 2016bLopezCruz et al, 2017;Zhang et al, 2016). Taken together, this supports the perspective that the Arabidopsis-B.…”

Section: Natural Genetic Variation In B Cinerea Influences Plant Trasupporting

confidence: 83%

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

et al. 2017

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To respond to pathogen attack, selection and associated evolution has led to the creation of plant immune system that are a highly effective and inducible defense system. Central to this system are the plant defense hormones jasmonic acid (JA) and salicylic acid (SA) and crosstalk between the two, which may play an important role in defense responses to specific pathogens or even genotypes. Here, we used the Arabidopsis thaliana-Botrytis cinerea pathosystem to test how the host's defense system functions against genetic variation in a pathogen. We measured defense-related phenotypes and transcriptomic responses in Arabidopsis wild-type Col-0 and JA-and SA-signaling mutants, coi1-1 and npr1-1, individually challenged with 96 diverse B. cinerea isolates. Those data showed genetic variation in the pathogen influences on all components within the plant defense system at the transcriptional level. We identified four gene coexpression networks and two vectors of defense variation triggered by genetic variation in B. cinerea. This showed that the JA and SA signaling pathways functioned to constrain/canalize the range of virulence in the pathogen population, but the underlying transcriptomic response was highly plastic. These data showed that plants utilize major defense hormone pathways to buffer disease resistance, but not the metabolic or transcriptional responses to genetic variation within a pathogen.

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Section: Natural Genetic Variation In B Cinerea Influences Plant Trasupporting

confidence: 83%

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

et al. 2017

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“…In addition, in our study, we found that the BcMtg2 deletion mutant exhibited decreased virulence in various plant tissue. A similar phenotype was also observed in a Rho3 GTPase deletion mutant in B. cinerea 32 . These consequences indicated that GTPases may have a similar function in the regulation of asexual reproduction, vegetative growth, survival mechanism and virulence in plant pathogenic fungi.…”

Section: Discussionsupporting

confidence: 74%

BcMtg2 is required for multiple stress tolerance, vegetative development and virulence in Botrytis cinerea

Shao

Zhang

Wang

et al. 2016

Sci Rep

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In Saccharomyces cerevisiae, the Mtg2 gene encodes the Obg protein, which has an important function in assembling ribosomal subunits. However, little is known about the role of the Obg GTPase in filamentous fungi. In this study, we identified an Mtg2 ortholog, BcMtg2, in B. cinerea. The BcMtg2 deletion mutant showed a defect in spore production, conidial germination and sclerotial formation. Additionally, the mutant increased sensitivity to various environmental stresses. The BcMtg2 mutant exhibited dramatically decreased virulence on host plant tissues. BcMtg2 mutant showed increased sensitivity to osmotic and oxidative stresses, and to Congo red (cell wall stress agent). In the yeast complement assay, growth defects of yeast BY4741ΔMTG2 mutant were partly restored by genetic complementation of BcMtg2 under these environmental stresses. Additionally, compared with the parental strain and complement strain, the BcMtg2 deletion mutant displayed a minor glycerol response to osmosis stress. These defective phenotypes were recovered in the complement strain ΔBcMtg2C, which was created by adding the wild-type BcMtg2 gene to the ΔBcMtg2 mutant. The results of this study indicate that BcMtg2 has a necessary role in asexual development, environmental stress response and pathogenicity in B. cinerea.

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“…Homologs of Rac have been identified in several filamentous fungi, but have not yet been found in B. cinerea (Takemoto et al, 2007). An et al (2015) reported that a small GTPAse, Rho3, a potential subunit of Nox complexes, was involved in the accumulation of ROS in hyphae tips and affected the sporulation, appressorium formation and virulence of B. cinerea. Interactions between Rho3 and other subunits of the Nox complexes are unknown.…”

Section: Reactive Oxygen Species and Nadph Oxidase Complexes: Many Inmentioning

confidence: 99%

Molecular aspects in pathogen-fruit interactions: Virulence and resistance

Tian

Torres

Ballester

et al. 2016

Postharvest Biology and Technology

146

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Fruit losses during postharvest storage and handling due to pathogen infections are one of the major problems in the global food chain supply. The application of chemical fungicides to control diseases is currently limited by legislation in some countries and also raises concerns about food and environmental safety. Exploring molecular aspects of pathogen-fruit interactions therefore has biological and economic significance as a means to help develop rational alternatives for disease control. In this review we present the current knowledge of molecular aspects in pathogen-fruit interactions, addressing the following topics: the application of new "omics" technologies for studying these interactions; the molecular mechanisms of fungal pathogen attack; the regulation of virulence by exogenous factors; and, finally, fruit defense mechanisms.

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Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea

Cited by 35 publications

References 45 publications

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

BcMtg2 is required for multiple stress tolerance, vegetative development and virulence in Botrytis cinerea

Molecular aspects in pathogen-fruit interactions: Virulence and resistance

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