Function of DNA synthesis and DNA repair in the survival of embryos during early germination and in dormancy

Elder, Rhoderick H.; Osborne, Daphne J.

doi:10.1017/s0960258500001550

Cited by 79 publications

(51 citation statements)

References 15 publications

(19 reference statements)

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“…Villiers and Edgcumbe (1975) showed that if oxygen is present, fully hydrated seeds of lettuce (Lactuca sativa) are able to repair chromosomal aberrations, provided germination is prevented. Similarly, repair of DNA was detected prior to germination in imbibed seeds of maize (Zea mays; Zlatanova et al, 1987) and pea (Pisum sativum; Onelli et al, 2000) and in embryos of wild oat (Avena fatua) and rye (Secale cereale) (Elder and Osborne, 1993;Boubriak et al, 1997). Seeds that are not quite fully hydrated are also capable of repairing damage (Ibrahim and Roberts, 1983).…”

Section: Introductionmentioning

confidence: 98%

Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage

et al. 2009

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† Background and Aims Most priming studies have been conducted on commercial seed lots of unspecified uniformity and maturity, and subsequent seed longevity has been reported to both increase and decrease. Here a seed lot of Digitalis purpurea L. with relatively uniform maturity and known history was used to analyse the effects of priming on seed longevity in air-dry storage. † Methods Seeds collected close to natural dispersal and dried at 15 % relative humidity (RH), 15 8C, were placed into experimental storage (60 % RH, 45 8C) for 14 or 28 d, primed for 48 h at 0, 21, 22, 25, 210 or 215 MPa, re-equilibrated (47 % RH, 20 8C) and then returned to storage. Further seed samples were primed for 2 or 48 h at 21 MPa and either dried at 15 % RH, 15 8C or immediately re-equilibrated for experimental storage. Finally, some seeds were given up to three cycles of experimental storage and priming (48 h at 21 MPa). † Key Results Priming at 21 MPa had a variable effect on subsequent survival during experimental storage. The shortest lived seeds in the control population showed slightly increased life spans; the longer lived seeds showed reduced life spans. In contrast, seeds first stored for 14 or 28 d before priming had substantially increased life spans. The increase tended to be greatest in the shortest lived fraction of the seed population. Both the period of rehydration and the subsequent drying conditions had significant effects on longevity. Interrupting air-dry storage with additional cycles of priming also increased longevity. † Conclusions The extent of prior deterioration and the post-priming desiccation environment affect the benefits of priming to the subsequent survival of mature seeds. Rehydration-dehydration treatments may have potential as an adjunct or alternative to the regeneration of seed accessions maintained in gene banks for plant biodiversity conservation or plant breeding.

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Section: Introductionmentioning

confidence: 98%

Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage

et al. 2009

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“…In embryos from dormant lines of Avena fatua, slow but continuous DNA replacement (repair) was observed. It was suggested that this maintained genome integrity, to distinguish it from replicative DNA synthesis associated with germination and growth (Elder and Osborne, 1993). However, it is not known whether other cell cycle related events also remain suppressed in imbibed dormant seeds.…”

Section: Introductionmentioning

confidence: 99%

Depth of dormancy in tomato (Lycopersicon esculentum Mill.) seeds is related to the progression of the cell cycle prior to the induction of dormancy

Castro¹,

Bino

Jing³

et al. 2001

Seed Sci. Res.

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Cell cycle activities are initiated following imbibition of non-dormant seeds. However, it is not known whether cell cycle related events other than DNA replication also remain suppressed in imbibed dormant seeds. The objective of this study was to demonstrate that the transitions between the non-dormant and dormant (both primary and secondary) states are reflected in cell cycle events, such as DNA replication and the changing patterns of the microtubular cytoskeleton involved in the processes of growth and development. The present studies were conducted on seeds from tomato (Lycopersicon esculentum cv. Moneymaker) that possessed primary dormancy or were manipulated to attain secondary dormancy. In addition, a non-dormant abscisic acid (ABA)-deficient mutant, sit w , was used. DNA replication, as measured by flow cytometry, and ␤-tubulin accumulation, analysed by immunoblotting, were compared with immunocytological studies of active DNA synthesis and microtubular cytoskeleton formation. It is shown that the depth of dormancy, which distinguishes primary and secondary dormancy, may depend on the progression of the cell cycle prior to the induction of dormancy.

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“…Image analysis scans, Elder and Osborne (1993) of the density of ethidium bromide fluorescence was made of the 1% alkaline agarose gel (Figure 2). S. D calculated for 6 replicates, P<0.001.…”

Section: Assessment Of Dna Integritymentioning

confidence: 99%

Natural Ultra Violet Radialtion on Field Grown Rice (Oryza sativa L.) Plants Confer Protection against Oxidative Stress in Seed during Storage under Subtropical Ambience

Choudhury¹,

Mandi²

2012

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While heat and humidity are known for causing oxidative macromolecular cell damage related seed deterioration during storage under subtropical conditions, information on effect of UV radiation that is also known to cause bio-molecular as well as direct hit damage in plants is largely lacking. A study involving measurements of UV irradiance in seed storage and also over open fields during seed maturation reveals an inverse correlation between genetically conferred (varietally expressed) content of non-enzymatic antioxidant viz flavonoids (functioning also as successors) and seed deterioration during post harvest storage. The data shows that extent of cell damage and thus seed viability is related to the varietal difference in flavonoids accumulation in mature rice seed. Low storer/viable varieties show lower content of flavonoids and higher oxidative damage in stored seed.

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Function of DNA synthesis and DNA repair in the survival of embryos during early germination and in dormancy

Cited by 79 publications

References 15 publications

Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage

Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage

Depth of dormancy in tomato (Lycopersicon esculentum Mill.) seeds is related to the progression of the cell cycle prior to the induction of dormancy

Natural Ultra Violet Radialtion on Field Grown Rice (Oryza sativa L.) Plants Confer Protection against Oxidative Stress in Seed during Storage under Subtropical Ambience

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