Function of complement factor H and imaging of small molecules by MALDI-MSI in a methamphetamine behavioral sensitization model

Xu, Jiamin; Zhang, Zhilin; Liu, Runzhe; Sun, Yi; Liu, Huihui; Nie, Zongxiu; Zhao, Xin; Pu, Xiaoping

doi:10.1016/j.bbr.2019.02.002

Cited by 8 publications

(5 citation statements)

References 54 publications

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“…ELISA kits for C3a (MBS268280) (53) and C5b-9 (MBS776999) (54) were purchased from MyBioSource. ELISA kit for factor H was from Cusbio (CSB-E08933m) (55). ELISA kit for C1-INH was from Novus (NBP2-70029).…”

Section: Methodsmentioning

confidence: 99%

Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function

et al. 2020

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Section: Methodsmentioning

confidence: 99%

Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function

et al. 2020

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“…Bodzon‐Kulakowska et al (2017) used DESI‐MSI to analyze brain slices from rats exposed to morphine, cocaine, and amphetamines. Xu et al examined specific regions of the brain by MALDI‐MSI to complement and validate the mechanism of methamphetamine addiction (J. Xu, Zhang, et al, 2019). The results showed that lipid levels were selectively regulated in central nervous system structures, indicating the severe influence of addictive drugs (Bodzon‐Kulakowska et al, 2017).…”

Section: Applications Of Msi In the Toxicological Analysis And Safety...mentioning

confidence: 99%

Advances in mass spectrometry imaging for toxicological analysis and safety evaluation of pharmaceuticals

Chen

Xie

et al. 2022

Mass Spectrometry Reviews

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Safety issues caused by pharmaceuticals have frequently occurred worldwide, posing a tremendous threat to human health. As an essential part of drug development, the toxicological analysis and safety evaluation is of great significance. In addition, the risk of pharmaceuticals accumulation in the environment and the monitoring of the toxicity from natural medicines have also received ongoing concerns. Due to a lack of spatial distribution information provided by common analytical methods, analyses that provide spatial dimensions could serve as complementary safety evaluation methods for better prediction and evaluation of drug toxicity. With advances in technical solutions and software algorithms, mass spectrometry imaging (MSI) has received increasing attention as a popular analytical tool that enables the simultaneous implementation of qualitative, quantitative, and localization without complex sample pretreatment and labeling steps. In recent years, MSI has become more attractive, powerful, and sensitive and has been applied in several scientific fields that can meet the safety assessment requirements. This review aims to cover a detailed summary of the various MSI technologies utilized in the biomedical and pharmaceutical area, including technical principles, advantages, current status, and future trends. Representative applications and developments in the safety‐related issues of different pharmaceuticals and natural medicines are also described to provide a reference for pharmaceutical research, improve rational clinical medicine use, and ensure public safety.

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“…Currently, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is an innovative methodology that enables label-free localization of molecular species in tissue sections mounted on electrically conductive slides (6). By means of unique advantages of label free, molecular specificity, high-throughput analysis, simple sample preparation, and in situ positioning (7), MALDI-MSI plays an irreplaceable role for measuring the spatial distribution of small molecules (8,9), metabolites (10), proteins (11), peptides (12), lipids (13), or drugs (14). Furthermore, recent improvements in MALDI-MSI have been shown to be useful for different kinds of cerebrovascular disease (8,15).…”

Section: Introductionmentioning

confidence: 99%

Effects of notoginseng leaf triterpenes on small molecule metabolism after cerebral ischemia/reperfusion injury assessed using MALDI–MS imaging

Wang¹,

Zhu²,

et al. 2021

Ann Transl Med

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Background: Notoginseng leaf triterpenes (PNGL) is believed to have neuroprotective effects via the inhibition of inflammatory response and neuronal apoptosis. However, its mechanisms underlying the antiischemia/reperfusion (I/R) injury effects on the regulation of small molecule metabolism in rat brain remains unclear. The purpose of this study was thus to explore the mechanisms of PNGL on the regulation of small molecule metabolism in rat brain after I/R injury using matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI).Methods: As a model of in vivo cerebral I/R injury, male Sprague-Dawley (SD) rats were established with a middle cerebral artery occlusion/reperfusion (MCAO/R) model after PNGL administration with 40 mg•kg -1 through intraperitoneal injection (i.p.) for 7 days. We assessed the neurological behavior, regional cerebral blood flow (r CBF), neuron injury, and spatial distribution of metabolic small molecules.Results: Our in vivo results suggested that PNGL increased cerebral blood flow and relieved neurological dysfunction. Furthermore, using MALDI-MSI, we demonstrated that PNGL regulated 16 endogenous small molecules implicated in metabolic networks including tricarboxylic acid (TCA) cycle, adenosine triphosphate (ATP) metabolism, malate-aspartate shuttle, metal ions, and antioxidants underwent noticeable changes after reperfusion for 24 h.Conclusions: PNGL is a novel cerebrovascular agent that can improve cerebral blood flow and attenuate adverse neurological disorders. The mechanisms are closely correlated with relative metabolic pathways, which offers insight into exploring new mechanisms in PNGL for the treatment of cerebral I/R injury.

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Function of complement factor H and imaging of small molecules by MALDI-MSI in a methamphetamine behavioral sensitization model

Cited by 8 publications

References 54 publications

Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function

Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function

Advances in mass spectrometry imaging for toxicological analysis and safety evaluation of pharmaceuticals

Effects of notoginseng leaf triterpenes on small molecule metabolism after cerebral ischemia/reperfusion injury assessed using MALDI–MS imaging

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