Function of anti‐<i>Strepfococcus mutans</i> antibodies: inhibition of virulence factors and enzyme neutralization

Gregory, Richard L.; Kindle, Janice C.; Hobbs, Laura C.; Filler, Steven J.; Malmström, Hans

doi:10.1111/j.1399-302x.1990.tb00643.x

Cited by 48 publications

(52 citation statements)

References 35 publications

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“…Those new functions of antibodies are interference of metabolic activity of infected cells (25,34) and neutralization of bacterial enzyme (14). The findings in the present study may represent one of the new functions of the antibody.…”

Section: Discussionmentioning

confidence: 76%

Blockade ofSalmonella enteritidisPassage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody

Sugita-Konishi

Ogawa

Arai

et al. 2000

Microbiology and Immunology

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Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. The antibodies inhibited the decrease in transepithelial electrical resistance (TEER) of the intestinal epithelial monolayers and IL-8 secretion of the cells induced by S.E. invasion. Also, the antibodies blocked the penetration of bacteria through the cell layer although they did not inhibit the growth of bacteria in the cells. Confocal microscopic photographs revealed the bacteria in the infected monolayer cells were bound to antibodies. These results indicate that anti-S.E. antibodies may protect the cells from destruction induced by S.E. invasion in intestinal epithelial cells in addition to the partial inhibitionof adhesion and invasion of S.E. at the cell surface. Passive antibodies against invasive bacteria would be useful to prevent the migration of S.E. to blood not only at the cell surface but also inside of intestinal epithelial cells.

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Section: Discussionmentioning

confidence: 76%

Blockade ofSalmonella enteritidisPassage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody

Sugita-Konishi

Ogawa

Arai

et al. 2000

Microbiology and Immunology

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“…Thus, prevents bacterial adhesion, colonization on tooth surface providing protection against dental caries [21][22][23][24][25][26]. In contrast studies conducted by Krasse B, et al (1987) reported salivary IgA levels and dental caries were inversely correlated .…”

Section: Discussionmentioning

confidence: 99%

Salivary proteins as biomarkers in dental caries: In vivo study

Nireeksha¹,

Hegde²,

N³

et al. 2017

Dent Oral Craniofac Res

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The aim of the study was to determine the relationship between salivary proteins and dental caries. 80 healthy adults were further divided into caries free (N=20) and caries active (N=60) based on DMFT index. Caries active was further divided into group I (D=1-3), group II (D=4-10), group III (D= >10).Saliva samples collected were further analysed for salivary total protein levels, salivary IgA levels, salivary mucin levels, salivary albumin/globulin ratio, salivary C-reactive protein levels and proline rich proteins. The data was then statistically analysed using one way ANOVA test and Post Hoc Tukey's test. P value < 0.05 was considered as statistically significant. Study showed significantly high levels of salivary total protein, salivary IgA and salivary albumin globulin ratio in caries free group. Salivary mucin and salivary C-reactive protein levels were significantly high in caries active group. 95% proline rich protein bands were present in caries free group, whereas caries active group showed 65% proline rich protein bands. This study adds to better understanding of salivary components and their role in dental caries. Modulation of these proteins could play an important role in determining the onset and progression of caries, opening a new avenue of caries prevention.

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“…It does not activate complement, but rather seems to promote aggregation (Liljemark et al, 1979). Specific slgA antibodies also may inhibit microbial enzymes involved in colonization or pathogenic processes (Gregory et al, 1990). Some oral bacteria produce an IgA protease which cleaves slgA in the hinge region.…”

Section: (Ii) Salivary Proteins-microbial Interactionsmentioning

confidence: 99%

Does Variability in Salivary Protein Concentrations Influence Oral Microbial Ecology and Oral Health?

Rudney

1995

Critical Reviews in Oral Biology & Medicine

113

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Salivary protein interactions with oral microbes in vitro include aggregation, adherence, cell-killing, inhibition of metabolism, and nutrition. Such interactions might be expected to influence oral ecology. However, inconsistent results have been obtained from in vivo tests of the hypothesis that quantitative variation in salivary protein concentrations will affect oral disease prevalence. Results may have been influenced by choices made during study design, including saliva source, stimulation status, control for flow rate, and assay methods. Salivary protein concentrations also may be subject to circadian variation. Values for saliva collected at the same time of day tend to remain consistent within subjects, but events such as stress, inflammation, infection, menstruation, or pregnancy may induce short-term changes. Long-term factors such as aging, systemic disease, or medication likewise may influence salivary protein concentrations. Such sources of variation may increase the sample size needed to find statistically significant differences. Clinical studies also must consider factors such as human population variation, strain and species differences in protein-microbe interactions, protein polymorphism, and synergistic or antagonistic interaction between proteins. Salivary proteins may form heterotypic complexes with unique effects, and different proteins may exert redundant effects. Patterns of protein-microbe interaction also may differ between oral sites. Future clinical studies must take those factors into account. Promising approaches might involve meta-analysis or multi-center studies, retrospective and prospective longitudinal designs, short-term measurement of salivary protein effects, and consideration of individual variation in multiple protein effects such as aggregation, adherence, and cell-killing.

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Function of anti‐Strepfococcus mutans antibodies: inhibition of virulence factors and enzyme neutralization

Cited by 48 publications

References 35 publications

Blockade ofSalmonella enteritidisPassage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody

Blockade ofSalmonella enteritidisPassage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody

Salivary proteins as biomarkers in dental caries: In vivo study

Does Variability in Salivary Protein Concentrations Influence Oral Microbial Ecology and Oral Health?

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