Function Enhancement of a Metabolic Module via Endogenous Promoter Replacement for Pseudomonas sp. JY-Q to Degrade Nicotine in Tobacco Waste Treatment

Li, Jun; Yi, Fengmei; Chen, Guoqing; Pan, Fanda; Yang, Yang; Shu, Ming; Chen, Zeyu; Zhang, Zeling; Mei, Xiaotong; Zhong, Weihong

doi:10.1007/s12010-021-03566-0

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…In addition, functional enhancement of a metabolic module via endogenous promoter replacement for Pseudomonas sp. JY-Q to degrade nicotine in tobacco waste treatment has been accomplished (Li et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Zhang,

Yin,

Lei

et al. 2023

AMB Expr

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A large number of nicotine-containing wastes produced during the tobacco manufacturing process are seriously harmful to the environment and human health. The degradation and transformation of nicotine-containing environmental contaminants to harmless substances has become an urgent requirement. Lasioderma serricorne can grow and reproduce in nicotine-rich sources, and their intestinal microbiota show promising potential to degrade and utilize nicotine. The purpose of this study is to screen and identify nicotine-degrading bacteria from the intestines of L. serricorne and explore their degradation characteristics. A dominant strain, YC7, with significant nicotine degradation capabilities was isolated from the intestines of L. serricorne. The strain was identified as Bacillus using a polyphasic approach. The test results showed it can produce multiple enzymes that include β-glucosidase, cellulase, proteases, and amylases. The nicotine-degrading bacteria were functionally annotated using databases. Nicotine dehydrogenase (NDH) was found by combining an activity tracking test and protein mass spectrometry analysis. The YC-7 NDH in the pathway was molecularly docked and functionally verified via the gene knockdown method. The binding ability of nicotine to nicotine-degrading enzymes was investigated using molecular docking. A high-efficiency nicotine-degrading bacteria, YC-7, was isolated and screened from tobacco, and the gene functions related to degradation were verified. This investigation provides a new hypothesis for screening nicotine-degrading bacteria and increases our knowledge of potential nicotine-degrading microbial sources.

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Section: Discussionmentioning

confidence: 99%

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Zhang,

Yin,

Lei

et al. 2023

AMB Expr

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“…However, in a complex of tobacco waste water extract, the effect of JY-Q on nicotine degradation was poor due to the lack of bacterial tolerance and nicotine conversion ability. To break through this bottleneck, researchers first identified the regulatory factors and promoters of the three modules (upper, middle, and downstream) of the nicotine metabolism in JY-Q and then applied metabolic engineering strategies for combined replacement of the endogenous promoters, and the nicotinic degradation rate of the modified bacterial strains increased by 67% and 69%, respectively, compared with the wild type [82,83].…”

Section: Application Of Nni-degrading Bacteria In the Remediation Of ...mentioning

confidence: 99%

Neonicotinoid Insecticide-Degrading Bacteria and Their Application Potential in Contaminated Agricultural Soil Remediation

Zeng,

Sun,

et al. 2024

Agrochemicals

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Recent advances in the microbial degradation of persistent organic pollutants have the potential to mitigate the damage caused by anthropogenic activities that are harmfully impacting agriculture soil ecosystems and human health. In this paper, we summarize the pollution characteristics of neonicotinoid insecticides (NNIs) in agricultural fields in China and other countries and then discuss the existing research on screening for NNI-degrading functional bacterial strains, their degradation processes, the construction of microbial consortia, and strategies for their application. We explore the current needs and solutions for improving the microbial remediation rate of NNI-contaminated soil and how these solutions are being developed and applied. We highlight several scientific and technological advances in soil microbiome engineering, including the construction of microbial consortia with a broad spectrum of NNI degradation and microbial immobilization to improve competition with indigenous microorganisms through the provision of a microenvironment and niche suitable for NNI-degrading bacteria. This paper highlights the need for an interdisciplinary approach to improving the degradation capacity and in situ survival of NNI-degrading strains/microbial consortia to facilitate the remediation of NNI-contaminated soil using strains with a broad spectrum and high efficiency in NNI degradation.

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Nicotine metabolism pathway in bacteria: mechanism, modification, and application

Zhang

Mei

et al. 2022

Appl Microbiol Biotechnol

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Function Enhancement of a Metabolic Module via Endogenous Promoter Replacement for Pseudomonas sp. JY-Q to Degrade Nicotine in Tobacco Waste Treatment

Cited by 3 publications

References 40 publications

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase

Neonicotinoid Insecticide-Degrading Bacteria and Their Application Potential in Contaminated Agricultural Soil Remediation

Nicotine metabolism pathway in bacteria: mechanism, modification, and application

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