Function and regulation of thermosensitive ion channel TRPV4 in the immune system

Acharya, Tusar Kanta; Sahu, Ram Prasad; Kumar, Satish; Kumar, Shamit; Rokade, Tejas Pravin; Chakraborty, Ranabir; Dubey, Nishant Kumar; Shikha, Deep; Chawla, Saurabh; Goswami, Chandan

doi:10.1016/bs.ctm.2022.07.002

Cited by 3 publications

(1 citation statement)

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“…Considering that TRPV4 is present in several tissues (including kidney and urinary balder which are critical in maintaining body’s Ca 2+ homeostasis) and cells (such as adipocytes, macrophages, T cells, chondrocytes, etc. ), the exact role of TRPV4 in bone mineralization is difficult to ascertain, especially using knock out animals ( Du et al, 2020 ; Acharya et al, 2022c ). Therefore, in this study, we explored the importance of TRPV4 in bone mineralization using osteoblast cell lines as well as primary cultures where TRPV4 is endogenously expressed.…”

Section: Introductionmentioning

confidence: 99%

TRPV4 regulates osteoblast differentiation and mitochondrial function that are relevant for channelopathy

Acharya

Pal

Ghosh

et al. 2023

Front. Cell Dev. Biol.

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Different ion channels present in the osteoblast regulate the cellular functions including bio-mineralization, a process that is a highly stochastic event. Cellular events and molecular signaling involved in such process is poorly understood. Here we demonstrate that TRPV4, a mechanosensitive ion channel is endogenously present in an osteoblast cell line (MC3T3-E1) and in primary osteoblasts. Pharmacological activation of TRPV4 enhanced intracellular Ca2+-level, expression of osteoblast-specific genes and caused increased bio-mineralization. TRPV4 activation also affects mitochondrial Ca2+-levels and mitochondrial metabolisms. We further demonstrate that different point mutants of TRPV4 induce different mitochondrial morphology and have different levels of mitochondrial translocation, collectively suggesting that TRPV4-mutation-induced bone disorders and other channelopathies are mostly due to mitochondrial abnormalities. These findings may have broad biomedical implications.

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Section: Introductionmentioning

confidence: 99%