Function and Regulation of the Plant COPT Family of High-Affinity Copper Transport Proteins

Puig, Sergi

doi:10.1155/2014/476917

Cited by 54 publications

(35 citation statements)

References 72 publications

(145 reference statements)

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“…Cu enters the plant through members of the CTR family of high--affinity Cu transport proteins, denoted as COPT in plants. The CTR/COPT protein family is conserved in eukaryotes and functions in Cu + uptake (Puig 2014). These proteins contain three transmembrane segments with the amino--terminus in the extracytosolic space and the carboxyl--terminus facing the cytosol.…”

Section: Introductionmentioning

confidence: 99%

Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.)

et al. 2017

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Copper deficiency and excess differentially affect iron homeostasis in rice and overexpression of the Arabidopsis high-affinity copper transporter COPT1 slightly increases endogenous iron concentration in rice grains. Higher plants have developed sophisticated mechanisms to efficiently acquire and use micronutrients such as copper and iron. However, the molecular mechanisms underlying the interaction between both metals remain poorly understood. In the present work, we study the effects produced on iron homeostasis by a wide range of copper concentrations in the growth media and by altered copper transport in Oryza sativa plants. Gene expression profiles in rice seedlings grown under copper excess show an altered expression of genes involved in iron homeostasis compared to standard control conditions. Thus, ferritin OsFER2 and ferredoxin OsFd1 mRNAs are down-regulated whereas the transcriptional iron regulator OsIRO2 and the nicotianamine synthase OsNAS2 mRNAs rise under copper excess. As expected, the expression of OsCOPT1, which encodes a high-affinity copper transport protein, as well as other copper-deficiency markers are down-regulated by copper. Furthermore, we show that Arabidopsis COPT1 overexpression (C1 ) in rice causes root shortening in high copper conditions and under iron deficiency. C1 rice plants modify the expression of the putative iron-sensing factors OsHRZ1 and OsHRZ2 and enhance the expression of OsIRO2 under copper excess, which suggests a role of copper transport in iron signaling. Importantly, the C1 rice plants grown on soil contain higher endogenous iron concentration than wild-type plants in both brown and white grains. Collectively, these results highlight the effects of rice copper status on iron homeostasis, which should be considered to obtain crops with optimized nutrient concentrations in edible parts.

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Section: Introductionmentioning

confidence: 99%

Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.)

et al. 2017

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“…When external bioavailable Cu levels are low, plants use an energy-consuming system by which Cu 2+ is reduced to Cu + by plasma membrane NADPH-dependent cupric reductases (Bernal et al 2012), and is then taken up through high-affinity COPT transporters. Three plasma membrane members of this family, COPT1, COPT2 and COPT6 (pmCOPT), are induced in Arabidopsis under Cu deficiency conditions (Sancenón et al 2003, Puig, 2014, Peñarrubia et al 2015. COPT1 is expressed mostly in the root apex and pollen, which indicates its participation in Cu + uptake from soil and redistribution to reproductive organs (Sancenón et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Interaction Between ABA Signaling and Copper Homeostasis inArabidopsis thaliana

et al. 2016

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ABA is involved in plant responses to non-optimal environmental conditions, including nutrient availability. Since copper (Cu) is a very important micronutrient, unraveling how ABA affects Cu uptake and distribution is relevant to ensure adequate Cu nutrition in plants subjected to stress conditions. Inversely, knowledge about how the plant nutritional status can interfere with ABA biosynthesis and signaling mechanisms is necessary to optimize stress tolerance in horticultural crops. Here the reciprocal influence between ABA and Cu content was addressed by using knockout mutants and overexpressing transgenic plants of high affinity plasma membrane Cu transporters (pmCOPT) with altered Cu uptake. Exogenous ABA inhibited pmCOPT expression and drastically modified COPT2-driven localization in roots. ABA regulated SPL7, the main transcription factor responsive for Cu deficiency responses, and subsequently affected expression of its targets. ABA biosynthesis (aba2) and signaling (hab1-1 abi1-2) mutants differentially responded to ABA according to Cu levels. Alteration of Cu homeostasis in the pmCOPT mutants affected ABA biosynthesis, transport and signaling as genes such as NCED3, WRKY40, HY5 and ABI5 were differentially modulated by Cu status, and also in the pmCOPT and ABA mutants. Altered Cu uptake resulted in modified plant sensitivity to salt-mediated increases in endogenous ABA. The overall results provide evidence for reciprocal cross-talk between Cu status and ABA metabolism and signaling.

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“…Accordingly it was found that the Fe-SOD gene was strongly induced in Arabidopsis while the Cu/Zn-SOD gene was down-regulated (Abdel-Ghany et al, 2005;Yamasaki et al, 2007;Cohu and Pilon, 2007). The molecular mechanisms of the downregulation of Cu-proteins, including Cu/Zn-SOD, are accomplished by expression of Cu-microRNAs which promote the degradation of respective transcripts of the Cu-proteins, as recently reviewed by Piug (2014).…”

Section: Copper Deficiencymentioning

confidence: 99%

Deficiency and toxicity of nanomolar copper in low irradiance—A physiological and metalloproteomic study in the aquatic plant Ceratophyllum demersum

et al. 2016

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a b s t r a c tEssential trace elements (Cu 2+ , Zn 2+ , etc) lead to toxic effects above a certain threshold, which is a major environmental problem in many areas of the world. Here, environmentally relevant sub-micromolar concentrations of Cu 2+ and simulations of natural light and temperature cycles were applied to the aquatic macrophyte Ceratophyllum demersum a s a model for plant shoots. In this low irradiance study resembling non-summer conditions, growth was optimal in the range 7.5-35 nM Cu, while PSII activity (F v /F m ) was maximal around 7.5 nM Cu. Damage to the light harvesting complex of photosystem II (LHCII) was the first target of Cu toxicity (>50 nM Cu) where Cu replaced Mg in the LHCII-trimers. This was associated with a subsequent decrease of Chl a as well as heat dissipation (NPQ). The growth rate was decreased from the first week of Cu deficiency. Plastocyanin malfunction due to the lack of Cu that is needed for its active centre was the likely cause of diminished electron flow through PSII ( PSII ). The pigment decrease added to the damage in the photosynthetic light reactions. These mechanisms ultimately resulted in decrease of starch and oxygen production.

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Function and Regulation of the Plant COPT Family of High-Affinity Copper Transport Proteins

Cited by 54 publications

References 72 publications

Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.)

Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.)

Interaction Between ABA Signaling and Copper Homeostasis inArabidopsis thaliana

Deficiency and toxicity of nanomolar copper in low irradiance—A physiological and metalloproteomic study in the aquatic plant Ceratophyllum demersum

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