Function and mechanism of polysaccharide on enhancing tolerance of Trichoderma asperellum under Pb2+ stress

Sun, Hao; Meng, Meng; Wu, Li; Zheng, Xiaomin; Zhu, Zhiwei; Dai, Shuhan

doi:10.1016/j.ijbiomac.2020.02.207

Cited by 14 publications

(7 citation statements)

References 42 publications

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“…XPS analysis exhibited a high proportion of polysaccharides in the Be9 cell surface. Polysaccharides have been described as playing an important role in enhancing the tolerance of microorganisms under metal stress ( Sun et al, 2020 ). In the cell surfaces of Pseudomonas putida similar percentages of peptides were obtained and were involved in the interaction with nZVI/Pd particles ( Lv et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Martínez-Rodríguez

Sánchez-Castro²,

Ojeda³

et al. 2023

Front. Microbiol.

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IntroductionIndustrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g., phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unraveled to optimize results when applying this remediation process.MethodsIn this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.).Results and DiscussionIt was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48 h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. These findings show the biotechnological use of this strain in the bioremediation of U as a function of phosphate substrate: U biomineralization (in a phosphate free system) and indirectly through the solubilization of orthophosphate from phosphate (P) containing waste products needed for U precipitation.

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Section: Discussionmentioning

confidence: 99%

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Martínez-Rodríguez

Sánchez-Castro²,

Ojeda³

et al. 2023

Front. Microbiol.

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“…In the correlation analysis (Table 1), there was a significant positive correlation (r = 0.8394, p < 0.01) between the polysaccharides and POD activity. Few studies have investigated the role of polysaccharides as part of a nonenzymatic antioxidant system in plant resilience, but several studies have shown that plant polysaccharides have antioxidant effects and mitigate heavy metal toxicity [49][50][51] .…”

Section: Mineral Element Uptakementioning

confidence: 99%

Antioxidant system response, mineral element uptake and safe utilization of Polygonatum sibiricum in cadmium-contaminated soil

Kang

Yang

Dai

et al. 2021

Sci Rep

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Chinese herbal medicine is widely cultivated in Southwest China, where the soil cadmium (Cd) contamination of farmland is more serious than that in China as a whole. In this study, Polygonatum sibiricum was exposed to Cd at concentrations of e−1, e0, e2, and e4 mg/kg for 30, 60, and 90 days, and the physiological stress responses, Cd and mineral element uptake, antioxidant enzyme activities, and content changes of pharmaceutical ingredients (polysaccharides) were analyzed to decipher the feasibility of safe utilization in Cd-contaminated soil. The results show that the activity of antioxidant enzymes (SOD and CAT) in the aboveground part was always higher than that in the underground part. The underground part of Polygonatum sibiricum mobilizes nonenzymatic systems to facilitate the synthesis of polysaccharides (PCP1, PCP2) with antioxidant properties to cope with Cd stress. Mineral elements (P, K, Ca, Mg, Fe, Cu, and Zn) significantly (p < 0.05) changed after 90 d of cultivation. In particular, the changes in the iron and zinc content were significantly correlated (p < 0.05) with the activities of SOD and POD. Soil Cd at e0 mg/kg can guarantee the safe production and utilization of Polygonatum sibiricum, and the stimulation of Cd promotes polysaccharide synthesis and biomass growth.

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“…From correlation analysis (Table .2), there was a signi cant positive correlation (r =0.8394, p < 0.01) between polysaccharides and POD activity. Fewer studies have investigated the role of polysaccharides as part of a non-enzymatic antioxidant system in plant resilience, but several studies have shown that plant polysaccharides have antioxidant effects and mitigate heavy metal toxicity [43][44][45] .…”

Section: Polysaccharide Content and Its Antioxidant Propertiesmentioning

confidence: 99%

Antioxidant Systems Response, Mineral Element Uptake and Safe Utilization of Polygonatum Sibiricum in Cadmium-Contaminated Soil

Kang

Dai

et al. 2021

Preprint

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Chinese herbal medicine is widely cultivated in southwest China where the soil cadmium (Cd) contamination of farmland is more serious than that in the whole of China. In this study, Polygonatum sibiricum were exposed to Cd at the concentrations of e− 1, e0, e2, and e4 mg·kg− 1 for up to 30,60, and 90 days and their physiological stress responses, Cd and mineral element uptake, antioxidant enzyme activities, and content changes of pharmaceutical ingredients (polysaccharides) were analyzed to decipher the feasibility of safety use in Cd contaminated soil. Results showed that the activity of antioxidant enzymes (SOD, POD, and CAT) of the aboveground part was enhanced in response to Cd stress after 90 d. Compared with the control, the underground part mobilizes non-enzymatic systems to facilitate the synthesis of polysaccharides (PCP1, PCP2) with antioxidant properties to cope with Cd stress. Mineral elements (P, K, Ca, Mg, Fe, Cu, and Zn) were significantly changed after 90 d of cultivation. In particular, the changes in iron and zinc contents were significantly correlated with the activities of SOD and POD. The soil Cd safety thresholds value for Polygonatum sibiricum is e0 mg·kg− 1, under which concentration the stimulation of Cd promotes polysaccharides synthesis and biomass growth.

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Function and mechanism of polysaccharide on enhancing tolerance of Trichoderma asperellum under Pb2+ stress

Cited by 14 publications

References 42 publications

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Antioxidant system response, mineral element uptake and safe utilization of Polygonatum sibiricum in cadmium-contaminated soil

Antioxidant Systems Response, Mineral Element Uptake and Safe Utilization of Polygonatum Sibiricum in Cadmium-Contaminated Soil

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