Function and glycosylation of plant-derived antiviral monoclonal antibody

Ko, Kisung; Tekoah, Yoram; Rudd, Pauline M.; Harvey, David J.; Dwek, Raymond A.; Spitsin, Sergei; Hanlon, Cathleen A.; Rupprecht, Charles E.; Dietzschold, Bernhard; Golovkin, Maxim; Koprowski, Hilary

doi:10.1073/pnas.0832472100

Cited by 232 publications

(183 citation statements)

References 45 publications

Supporting

Mentioning

164

Contrasting

Unclassified

Order By: Relevance

“…In the nude mice experiment, both mAb P and mAb M fully protected mice from tumor growth up to 25 days. However, after 25 days the mAb P -treated mice showed some increase in tumor growth, which could be the result of a shorter half-life of plant mAb P , which itself could be due to the type of glycosylation (high-mannose) associated with the addition of the ER retention KDEL motif (13). On the other hand, this fucose-deficient type of glycosylation of mAb P BR55-2 did not perturb the in vitro interaction between the Fc of the mAb and the Fc receptor (27,28).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Plant-derived anti-Lewis Y mAb exhibits biological activities for efficient immunotherapy against human cancer cells

Brodzik

Glogowska²,

Bandurska³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Although current demands for therapeutic mAbs are growing quickly, production methods to date, including in vitro mammalian tissue culture and transgenic animals, provide only limited quantities at high cost. Several tumor-associated antigens in tumor cells have been identified as targets for therapeutic mAbs. Here we describe the production of mAb BR55-2 (IgG2a) in transgenic plants that recognizes the nonprotein tumor-associated antigen Lewis Y oligosaccharide overexpressed in human carcinomas, particularly breast and colorectal cancers. Heavy and light chains of mAb BR55-2 were expressed separately and assembled in plant cells of low-alkaloid tobacco transgenic plants (Nicotiana tabacum cv. LAMD609). Expression levels of plant-derived mAb (mAb P ) were high (30 mg͞kg of fresh leaves) in T 1 generation plants. Like the mammalian-derived mAb M , the plant mAb P bound specifically to both SK-BR3 breast cancer cells and SW948 colorectal cancer cells. The Fc domain of both mAb P and mAb M showed the similar binding to Fc␥RI receptor (CD64). Comparable levels of cytotoxicity against SK-BR3 cells were also shown for both mAbs in antibody-dependent cell-mediated cytotoxicity assay. Furthermore, plant-derived BR55-2 efficiently inhibited SW948 tumor growth xenografted in nude mice. Altogether, these findings suggest that mAb P originating from low-alkaloid tobacco exhibit biological activities suitable for efficient immunotherapy.breast and colorectal cancer ͉ plant biotechnology ͉ transgenic low-alkaloid tobacco ͉ tumor growth inhibition

show abstract

Section: Discussionmentioning

confidence: 99%

“…Moreover, plants offer several advantages as a mAb production system, such as the lack of human pathogens, relatively low-cost manufacturing, and ease of production scale-up. Our group has recently shown that recombinant mAb can be safely purified from tobacco plants (13).…”

mentioning

confidence: 99%

Plant-derived anti-Lewis Y mAb exhibits biological activities for efficient immunotherapy against human cancer cells

Brodzik

Glogowska²,

Bandurska³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…To generate HA1-Fc fusions (HA1-Fc), cDNA clones encoding the heavy chain of human anti-rabies monoclonal antibody SO57 [7] and the heavy chain of murine antibody CO17-1A [8] were used as PCR templates.…”

Section: Expression Of Ha1 Variants In Plantsmentioning

confidence: 99%

“…HA-Fc fusion proteins were purified in a single-step protocol using protein A beads as described [7]. In vitro characterization and quantification of protein expression were done by ELISA and Western blot analysis as described [6,7].…”

Section: Isolation and Purification Of Recombinant Proteinsmentioning

confidence: 99%

Immunological assessment of plant-derived avian flu H5/HA1 variants

Spitsin

Andrianov

Pogrebnyak

et al. 2009

Vaccine

View full text Add to dashboard Cite

“…In high-risk patient of colorectal cancer, the mAb CO17-1A is efficacious for the prevention of micro metastasis and recurrent of colorectal cancer (Riethmuller et al 1998). In addition, their previous studies have recently reported that recombinant mAb CO17-1A has been expressed in tobacco plant (Verch et al 1998) and that recombinant mAb can be safely purified from tobacco plant (Ko et al 2003). However, anti-inflammatory effects of plant-derived multiple monoclonal antibody CO17-1A × BR55 (multiple mAb P CO17-1A × BR55) related to anti-cancer in AOM/DSS-induced colorectal cancer animal model are unclear.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory actions of plant-derived multiple monoclonal antibody CO17-1A × BR55 related with anti-cancer effects in AOM/DSS-induced colorectal cancer mouse via down-regulating of ERK1/2

Kwak

Heo

Kim

et al. 2016

Animal Cells and Systems

View full text Add to dashboard Cite

Plant-derived multiple monoclonal antibody (mAb) CO17-1A × BR55 (mAb P CO17-1A × BR55) produced in transgenic tobacco plants were cross-pollinated with mAb CO17-1A and mAb BR55. Human anti-colorectal cancer multiple mAb CO17-1A × BR55 was cloned using pBI121 vector. Mice were given a single intraperitoneal injection of azoxymethane (AOM) with 10 mg/kg body weight. Starting 1 week after the injection, mice received 2% dextran sulfate sodium (DSS) in the drinking water for 1 week. In addition, the mice were injected intraperitoneal with mAbs dissolved in phosphate buffered saline (100 μg/mouse) twice per week for 4 weeks. Apoptotic cell death, expression of pro-apoptotic proteins, activity of inflammatory cytokines and ERK pathway phosphorylation were assayed by Western blot and TUNEL kit. mAb P CO17-1A × BR55 meaningfully and efficiently suppressed the development of AOM/DSS-induced colorectal inflammation and colorectal tumors, as determined by a reduced activation of inflammatory cytokines, number of colorectal tumor-induced mouse, number of tumor per mouse colon than other mAbs. Cell death by apoptosis was much increased in the mAb P CO17-1A × BR55-treated tumor compared with negative control. Apoptotic cell death and expression of pro-apoptotic proteins including Bax and cleaved caspase-3 were highest in treatment with mAb P CO17-1A × BR55. In addition, mAbP CO17-1A × BR55 was meaningfully decreased the expression of inflammatory cytokines, including COX-2, iNOS, p50 and p65, but the expression of PPARγ was significantly increased compared with AOM/DSS-induced carcinogenesis negative control. Moreover, mAb P CO17-1A × BR55 meaningfully repressed the ERK1/2 phosphorylation in AOM/ DSS-induced colorectal tumors. Therefore, our results suggest that multiple mAb P CO17-1A × BR55 have meaningful effects of anti-inflammation related with the anti-carcinogenesis in AOM/ DSS-induced colorectal tumor by inhibition of ERK1/2 phosphorylation. ARTICLE HISTORY

show abstract

Function and glycosylation of plant-derived antiviral monoclonal antibody

Cited by 232 publications

References 45 publications

Plant-derived anti-Lewis Y mAb exhibits biological activities for efficient immunotherapy against human cancer cells

Plant-derived anti-Lewis Y mAb exhibits biological activities for efficient immunotherapy against human cancer cells

Immunological assessment of plant-derived avian flu H5/HA1 variants

Anti-inflammatory actions of plant-derived multiple monoclonal antibody CO17-1A × BR55 related with anti-cancer effects in AOM/DSS-induced colorectal cancer mouse via down-regulating of ERK1/2

Contact Info

Product

Resources

About