Fun Microbiology: Using a Plant Pathogenic Fungus to Demonstrate Koch's Postulates

Mitchell, James K.; Orsted, Kathy M.; Warnes, Carl E.

doi:10.2307/4450385

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Others have used plants to demonstrate Koch's postulates in a teaching laboratory (Fulton, 1981;Hogue, 1971;Lennox, 1985;Mitchell et al, 1997;Ringel, 1968). However, we wanted to simulate the original generation of these postulates as formulated by Robert Koch while isolating Bacillus anthracis from sheep to induce anthrax in rabbits (Brock, 1999).…”

Section: Introductionmentioning

confidence: 99%

A Microbiology Teaching Lab: Using Koch's Postulates to Determine the Cause of “Peep Pox” in Marshmallow Peeps

Dahl

Gatlin

2018

The American Biology Teacher

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Koch's postulates are regularly included in the lecture portion of microbiology courses, but rarely are they demonstrated in a microbiology teaching lab. This is understandable given the logistical challenges of undergraduates working with pathogenic bacteria, ethical concerns using animals, and limited time constraints of a weekly lab period. Here we present a cost-effective, time-friendly lab activity that demonstrates the principles of microbial isolation and infection assays that are part of fulfilling Koch's postulates. The disease is “peep pox” caused by a gelatinase-positive bacterial species hydrolyzing marshmallow peeps that proxy as infected animals.

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Section: Introductionmentioning

confidence: 99%

A Microbiology Teaching Lab: Using Koch's Postulates to Determine the Cause of “Peep Pox” in Marshmallow Peeps

Dahl

Gatlin

2018

The American Biology Teacher

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Novel Isoxazolylpyrimidine Derivatives: Design, Synthesis, Antifungal Activity and In‐Silico Studies

Nagaraja rao,

Poojary,

Mohan Kumar

et al. 2024

Asian J Org Chem

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The advancement of environmentally friendly chemical synthesis in both academic and corporate research has recently taken on enormous relevance. Pesticides are substances, either chemical or biological, that are used to get rid of or lessen the impact of pests. To control the fungi in crops a new set of isoxazolylpyrimidine derivatives were planned, synthesized, and characterized. The compounds indicated were synthesized in high yields, and their structures were determined using elemental analysis, 1H‐NMR, 13C NMR, and LCMS. HPLC was used to assess the purity of the synthesized molecules, and the purity of some compounds were found to be >98% a/a while a couple were found to be >95% a/a. The synthesized compounds were assessed for their antifungal activity. Among these, compounds 5d, 5h, and 5l exhibit substantial antifungal action and considerably suppress the growth of Rhizoctonia solani, Sclerotium rolfsii, and Macrophominaphaseolina. The plausible mechanism of fungal inhibitory activities of rationally designed isoxazolyl pyrimidine derivatives is attributed to the blocking of the oxygen activation of heme Iron in membrane‐bound 14 alpha‐demethylase. The presence of pyrimidine unit in designed materials is a vital factor for inducing antifungal activities, which was further corroborated through the density functional theory (DFT) and molecular docking studies.

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Exhaustive identification and characterization of Colletotrichum siamense and Colletotrichum fructicola as causative agents of circular leaf spot disease of rubber tree (Hevea brasiliensis) in India

Vineeth,

Babu,

Reshma

et al. 2024

Fungal Biology

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Fun Microbiology: Using a Plant Pathogenic Fungus to Demonstrate Koch's Postulates

Cited by 3 publications

References 6 publications

A Microbiology Teaching Lab: Using Koch's Postulates to Determine the Cause of “Peep Pox” in Marshmallow Peeps

A Microbiology Teaching Lab: Using Koch's Postulates to Determine the Cause of “Peep Pox” in Marshmallow Peeps

Novel Isoxazolylpyrimidine Derivatives: Design, Synthesis, Antifungal Activity and In‐Silico Studies

Exhaustive identification and characterization of Colletotrichum siamense and Colletotrichum fructicola as causative agents of circular leaf spot disease of rubber tree (Hevea brasiliensis) in India

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