Fumonisinas –Síntesis y función en la interacción Fusarium verticillioides-maíz

Torre-Hernández, Ma. Eugenia de la; Sánchez-Rangel, Diana; Galeana-Sánchez, Eduardo; Parra, Javier Plasencia-de la

doi:10.1016/s1405-888x(14)70321-3

Cited by 16 publications

(6 citation statements)

References 97 publications

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“…A filamentous fungus strain EA-FP0013 was successfully isolated in PDA medium from infected sugarcane tissue, whose characteristics coincide with the most representative characters of the strains of Fusarium verticillioides reported in the literature by [16], [17], [18], [19], [20]. The isolated fungus presents mycelial growth with colorations between pink, purple and red in the center with white edges ( Figure 1A), radial growth, oval microconidia with flattened base and grouped in chains ( Figure 1B and 1D).…”

Section: Identification and Morphological Characterization Of Isolatesupporting

confidence: 64%

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

et al. 2018

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The cultivation of sugarcane represents an important part in the Colombian economy due to the diverse applications in industries like liquor, food, paper and biofuels. Sugarcane worldwide production is affected by the presence of phytopathogenic agents, mainly filamentous fungi such as Physalospora tucumanensis (red rot disease) and Fusarium spp. To date in Colombia, Pokka boheng disease whose causal agent is the fungus Fusarium verticillioides, has not been reported, which is why it is necessary to identify appropriately this microorganism, responsible for losses in productivity and food contamination. In order to isolate and identify the infectious agent from symptomatic tissues, disinfection and culture in liquid and solid culture mediums were performed in malt extract (2%) and yeast extract (0.2%) both liquid and solid, for 7 days. After several replicates in agar plate, a purification was made along with a morphological characterization based on the shape and color of the mycelium, as well as the type of spores generated. Additionally, the genetic material was extracted and gene markers (ITS, Elongation Factor 1-α (EF) and β-Tubulin (Btub)) were amplified by PCR. Then, DNA sequencing was used to obtain the data to make a phylogenetic reconstruction by probabilistic methods (Maximum Likelihood and Bayesian Inference). The isolated strain, named as EA-FP0013 was located in the Fujikuroi complex group, with high probable identity to Fusarium verticillioides. Thus, early and species-specific identification of these fungal isolates by molecular methods may allow the timely diagnosis of emerging pathophysiological diseases of interest in the region, and thus propose the respective control strategies.

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Section: Identification and Morphological Characterization Of Isolatesupporting

confidence: 64%

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

et al. 2018

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“…Gibberella moniliformis was isolated from four symptomatic seed samples; in this sense, the races "Elotes occidentales 1" and "Arrocillo 1" presented incidence values of 83.3% and 66.7%, respectively. That fungal species has been reported in Mexico as a pathogen of great importance, affecting different corn parts: cob, grains, root and stem (10). Gibberella moniliformis was also detected in a sample of asymptomatic seed fragments of race "Cónico Pepitilla", showing incidence values of 41.7% (Table 2).…”

Section: Resultsmentioning

confidence: 78%

Frequency of fungi associated with Creole corn seeds under different environmental conditions in Guerrero, Mexico

et al. 2022

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In Mexico, Creole corn has presented problems related to seed rot. Therefore, the objective of this study was to determine the frequency of fungi associated with Creole corn seeds. From January to February 2019, 13 Creole corn samples were collected from several localities in Guerrero, Mexico. The samples were processed in PDA culture medium and the frequency of each fungus was estimated based on cultural characteristics and taxonomic keys. Considering symptomatic corn seed samples, Fusarium oxysporum was recorded in eight samples at 97.2%, followed by F. graminearum, isolated from race “Olotillo 1” at 94.4% and from race “Cónico pepitilla” at 58.3%. Phomopsis sp. was isolated at 100% from race “Olotillo 2”. Gibberella moniliformis was isolated from “Elotes Occidental 1” at 83.3%, while the least frequent isolate (19.4%) from “Pepitilla” was Sarocladium strictum, which was recorded in three samples at the low values of 11.1% and 2.8% for races “Arrocillo 2” and “Pepitilla”, respectively; Talaromyces variabilis was found in two samples at 11.1% and 2.8% from “Arrocillo 2” and “Cónico pepitilla”, respectively; Stenocarpella macrospora was isolated at 72.2% from “Pepitilla”. Aspergillus flavus was obtained from “Cónico Pepitilla” at 16.7%, while Setosphaeria turcica and Emericella sp. were less frequently isolated. Considering asymptomatic seeds of race “Cónico pepitilla”, F. oxysporum was isolated at 8.3%, Fusarium acuminatum at 8.3% and Gibberella moniliformis at 41.7%; from asymptomatic seeds of “Olotillo 1” and “Olotillo 2”, Phomopsis sp. was isolated at 50% and Rhizoctonia solani at 50%, respectively. In the present study, the fungi F. oxysporum, Phomopsis sp., G. moniliformis and F. graminearum were most frequently associated with Creole corn seeds.

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“…Los aislamientos de F. verticillioides difirieron en las características del desarrollo de su micelio y color del mismo. Las variantes de pigmentación del micelio después de 8-10 días de crecimiento en caja Petri coinciden con lo informado por otros autores (de la Torre-Hernández et al 2014). Las cepas de F. verticillioides que producen tonalidades violetas en su micelio presentan una elevada virulencia en plántulas de maíz, al reducir de manera notable el desarrollo de la raíz (Solano-Báez et al, 2011).…”

Section: Discussionunclassified

“…La incidencia de la enfermedad no mostró diferencia significativa en dieciocho de los veinte aislamientos y el testigo (Figura 5A), lo que se atribuye a que el hongo es cosmopolita y se presenta en todos los sitios donde se cultiva maíz (Naz et al, 2021), por lo que de manera natural infectó la parcela testigo. Ahora bien, las diferencias significativas en la SDI observadas entre varias de las cepas y el testigo, podrían deberse a la capacidad de cada cepa para producir enzimas líticas y toxinas que facilitan el proceso infeccioso (de la Torre-Hernández et al, 2014).…”

Section: Discussionunclassified

Variabilidad morfológica/patogénica de Fusarium verticillioides en la Ciénega/Chapala, México y evaluación de técnicas de inoculación

et al. 2022

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Introducción. La pudrición de mazorca en maíz causada por Fusarium verticillioides es una enfermedad de las más importantes en el mundo. En México ocasiona pérdidas de hasta 30 %. El incremento en los niveles de esta enfermedad en los últimos años requiere inspecciones en las zonas productoras para identificar las principales cepas que afectan el grano de maíz. Objetivo. Determinar la variabilidad morfológica y patogénica de aislados de F. verticillioides de muestras de maíz de la zona productora de maíz La Ciénega/Chapala, México, e identificar la mejor técnica de inoculación del patógeno en mazorcas. Materiales y métodos. Se obtuvieron veinte aislamientos de F. verticillioides de muestras de granos de maíz con síntomas de la enfermedad. Los aislamientos se usaron para inocular, mediante diferentes técnicas, mazorcas en desarrollo (R2) del maíz P-3057 cultivado en Tepatitlán, Jalisco en el ciclo Primavera/Verano 2019 bajo condiciones de temporal. Se evaluó la incidencia y severidad de la infección (SDI) de los aislamientos y la morfología microscópica de las cepas. Resultados. Los aislamientos de F. verticillioides difirieron en color de la colonia, tipo de micelio, abundancia y coloración del micelio vegetativo. La incidencia y severidad fueron estadísticamente diferentes entre los aislamientos de F. verticillioides y las técnicas de inoculación probadas, con interacción entre aislamientos y técnicas de inoculación. La incidencia de la enfermedad mostró valores de 82 y 95 % con las técnicas de inoculación por aspersión y del palillo, respectivamente. La SDI varió entre 8 % y 15,5 % con las técnicas de inoculación por aspersión y palillo, en ese orden. Conclusiones. Las cepas de F. verticillioides mostraron variabilidad para patogenicidad y severidad de la infección. La patogenicidad se relacionó con la técnica de inoculación utilizada. La técnica de inoculación del palillo fue la que presentó mayor incidencia y severidad de la infección.

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Fumonisinas –Síntesis y función en la interacción Fusarium verticillioides-maíz

Cited by 16 publications

References 97 publications

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

Frequency of fungi associated with Creole corn seeds under different environmental conditions in Guerrero, Mexico

Variabilidad morfológica/patogénica de Fusarium verticillioides en la Ciénega/Chapala, México y evaluación de técnicas de inoculación

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