Fumonisin B1 analysis in maize by Molecularly Imprinted Polymer Paper Spray Ionization Mass Spectrometry (MIP-PSI-MS)

Ramalho, Ruver Rodrigues Feitosa; Silva, Wellington Alves Mizael da; Lima, Gesiane da Silva; Santos, Glauber Eduardo de Oliveira; Maciel, Lanaia Ítala Louzeiro; Simas, Rosineide C.; Vaz, Boniek Gontijo

doi:10.1016/j.jfca.2021.104362

Cited by 12 publications

(4 citation statements)

References 32 publications

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“…Tyrosinase is the most widely distributed enzyme, with copper as its main constituent, belonging to the class of the type 3 copper enzyme family, and is involved in the formation of melanin pigment in a number of organisms. The second docking experiment was carried out on human peroxiredoxin 5 (PDB code 1HD2), which belongs to the widely expressed six-member peroxiredoxin family of antioxidant enzymes [ 29 ]. Peroxiredoxins reduce hydrogen peroxide and alkyl hydroperoxides by reducing equivalents from thiol-containing donor molecules, for example, trypanothione, thioredoxin, glutathione, and AhpF, and they are found in both prokaryotes and eukaryotes.…”

Section: Resultsmentioning

confidence: 99%

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Assessing the Effectiveness of Chemical Marker Extraction from Amazonian Plant Cupuassu (Theobroma grandiflorum) by PSI-HRMS/MS and LC-HRMS/MS

Lima

Santos

et al. 2023

Metabolites

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Employing a combination of liquid chromatography electrospray ionization and paper spray ionization high-resolution tandem mass spectrometry, extracts from cupuassu (Theobroma grandiflorum) pulp prepared with either water, methanol, acetonitrile or combinations thereof were subjected to metabolite fingerprinting. Among the tested extractors, 100% methanol extracted preferentially phenols and cinnamic acids derivatives, whereas acetonitrile and acetonitrile/methanol were more effective in extracting terpenoids and flavonoids, respectively. And while liquid chromatography- mass spectrometry detected twice as many metabolites as paper spray ionization tandem mass spectrometry, the latter proved its potential as a screening technique. Comprehensive structural annotation showed a high production of terpenes, mainly oleanane triterpene derivatives. of the mass spectra Further, five major metabolites with known antioxidant activity, namely catechin, citric acid, epigallocatechin-3′-glucuronide, 5,7,8-trihydroxyflavanone, and asiatic acid, were subjected to molecular docking analysis using the antioxidative enzyme peroxiredoxin 5 (PRDX5) as a model receptor. Based on its excellent docking score, a pharmacophore model of 5,7,8-trihydroxyflavanone was generated, which may help the design of new antioxidants.

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Section: Resultsmentioning

confidence: 99%

“…PSI-MS analysis was performed using previously described protocols [ 27 , 28 , 29 ]. The membrane was cut into an equilateral triangle with 1 cm sides and held by a metal clip connected to the voltage source of the mass spectrometer.…”

Section: Methodsmentioning

confidence: 99%

Assessing the Effectiveness of Chemical Marker Extraction from Amazonian Plant Cupuassu (Theobroma grandiflorum) by PSI-HRMS/MS and LC-HRMS/MS

Lima

Santos

et al. 2023

Metabolites

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“…However, these techniques require complex procedures, high solvent consumption, and expensive instrumentation. Accordingly, Ramalho et al [ 59 ] proposed a new method of MIP combined with PSI-MS to improve the sensitivity of FB 1 analysis in maize samples ( Figure 4 ). The precision and accuracy of all analyses are below 5.5%.…”

Section: Paper-based Molecular-imprinting Technologymentioning

confidence: 99%

Paper-Based Molecular-Imprinting Technology and Its Application

Liu

2022

Biosensors

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Paper-based analytical devices (PADs) are highly effective tools due to their low cost, portability, low reagent accumulation, and ease of use. Molecularly imprinted polymers (MIP) are also extensively used as biomimetic receptors and specific adsorption materials for capturing target analytes in various complex matrices due to their excellent recognition ability and structural stability. The integration of MIP and PADs (MIP-PADs) realizes the rapid, convenient, and low-cost application of molecular-imprinting analysis technology. This review introduces the characteristics of MIP-PAD technology and discusses its application in the fields of on-site environmental analysis, food-safety monitoring, point-of-care detection, biomarker detection, and exposure assessment. The problems and future development of MIP-PAD technology in practical application are also prospected.

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“…Regulatory agencies worldwide have established maximum residue limits (MRLs) for pesticides in food commodities to enforce food safety for end customers. MRLs are the maximum tolerated level of pesticide residues in food or feed regulated by agencies. − Currently, the European Union (EU) legislation has determined the maximum level of TBZ in fruits and vegetables in the range of 0.05–15 ppm . In Brazil, the ANVISA (National Health Surveillance Agency) sets the MRL at 0.01–10 ppm, and the Chinese authority establishes the MRL at 20 ppm …”

Section: Introductionmentioning

confidence: 99%

Combining LAESI Imaging and Tissue Spray Ionization Mass Spectrometry To Unveil Pesticides Contaminants in Fruits

Lima,

Pereira,

Maciel

et al. 2023

J. Am. Soc. Mass Spectrom.

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There is an increasing need for developing a strategy to analyze the penetration of pesticides in cultures during postharvest control with minimal or no sample preparation. This study explores the combined use of laser ablation electrospray ionization mass spectrometry imaging (LAESI imaging) and tissue spray ionization mass spectrometry (TSI-MS) to investigate the penetration of thiabendazole (TBZ) in fruits, simulating a postharvest procedure. Slices of guava and apple were prepared, and an infrared laser beam was used, resulting in the ablation of TBZ directly ionized by electrospray and analyzed by mass spectrometry. The experiments were conducted for 5 days of fruit storage after TBZ administration to simulate a postharvest treatment. During postharvest treatment, TBZ is applied directly to the fruit peel after harvesting. Consequently, TBZ residues may remain on the peel if the consumer does not wash the fruit properly before its consumption. To evaluate the effectiveness of household washing procedures, TSI-MS was employed as a rapid and straightforward technique to monitor the remaining amount of TBZ in guava and apple peels following fruit washing. This study highlights the advantages of LAESI imaging for evaluating TBZ penetration in fruits. Moreover, the powerful capabilities of TSI-MS are demonstrated in monitoring and estimating TBZ residues after pesticide application, enabling the comprehensive unveiling of pesticide contaminants in fruits.

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Fumonisin B1 analysis in maize by Molecularly Imprinted Polymer Paper Spray Ionization Mass Spectrometry (MIP-PSI-MS)

Cited by 12 publications

References 32 publications

Assessing the Effectiveness of Chemical Marker Extraction from Amazonian Plant Cupuassu (Theobroma grandiflorum) by PSI-HRMS/MS and LC-HRMS/MS

Assessing the Effectiveness of Chemical Marker Extraction from Amazonian Plant Cupuassu (Theobroma grandiflorum) by PSI-HRMS/MS and LC-HRMS/MS

Paper-Based Molecular-Imprinting Technology and Its Application

Combining LAESI Imaging and Tissue Spray Ionization Mass Spectrometry To Unveil Pesticides Contaminants in Fruits

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