Fumaric Acid, Anti Thrombin Substance from Rhizopus javanicus.

Okamura, Tokumitsu; Horie, Noboru; Miyazaki, Yoshiko; Ohsugi, Masahiro

doi:10.3177/jnsv.43.241

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…Finally, the compound 1 (Figure 1) was isolated and confirmed to have antibacterial activity. The results of mass spectrometry (Table 2), 1 H-NMR, 13 C-NMR (Table 3) and IR spectra (Table 4) showed good concordance with the spectrum data of fumaric acid reported by Okamura et al (1997).…”

Section: Chemical Groupsupporting

confidence: 72%

Fumaric acid, an antibacterial component of Aloe vera L.

He¹,

Fu#²,

Shen³

et al. 2011

Afr. J. Biotechnol.

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The acetoacetate fraction partitioned from Aloe vera L. showed an effective antibacterial activity against four clinically frequent pathogenic bacteria. Through a series of chromatographic methods and activity assays, one compound was obtained and it has potent antibacterial activity. Based on the data of mass spectrometry, 1 H-NMR, 13C-NMR and IR spectra, this compound was determined to be fumaric acid. This study concluded that fumaric acid is one of the antibacterial components in A. vera.

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Section: Chemical Groupsupporting

confidence: 72%

Fumaric acid, an antibacterial component of Aloe vera L.

He¹,

Fu#²,

Shen³

et al. 2011

Afr. J. Biotechnol.

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show abstract

“…In previous studies, the antiplatelet aggregation activity of malic acid and critic acid was reported [36], and the activity of rutin involved antiplatelet aggregation and anti‐thrombotic [37–38], while vitexin‐2″‐ O ‐rhamnoside and rutin from the extract of Crataegi Folium had the effect of decreasing the WBV in blood stasis rats [39]. In addition, the anti‐thrombin activity of fumaric acid from Rhizopus javanicus was reported [40]. These reports showed that the potential blood activating components screened in this presented study were very likely bioactive components of hawthorn.…”

Section: Resultsmentioning

confidence: 99%

Combining ultra‐high‐performance liquid chromatography quadruple exactive orbitrap mass spectrometry with chemometrics to identify and verify the blood‐activating components of hawthorn

Sun

et al. 2022

J of Separation Science

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Hawthorn, one of the widely‐used Chinese herbal medicines, has been used to treat blood stasis syndrome in the clinic, but its blood‐activating components are unclear. This study combined the ultra‐high‐performance liquid chromatography‐quadruple exactive‐orbitrap mass spectrometry with chemometrics to identify the blood‐activating components of hawthorn. Different polar fractions of hawthorn aqueous extracts were extracted and mixed to prepare 14 samples. The contents of 25 chemical components for 14 samples were determined by the proposed quantitative method which was validated in terms of linearity, precision, stability, repeatability, and recovery, while the blood‐activating effect was evaluated by measuring the whole blood viscosity, plasma viscosity, and plasma fibrinogen levels. Then the partial least squares model was established on the spectrum‐effect relationship. The result showed that vitexin‐2″‐O‐rhamnoside, rutin, citric acid, malic acid, gallic acid, and fumaric acid could reduce the whole blood viscosity, plasma viscosity, and plasma fibrinogen levels in blood stasis model rats, and these components were the blood‐activating components of hawthorn. This study provided a scientific basis for clarifying the blood‐activating components of hawthorn, and the spectrum‐effect approach proved to be an effective approach to discovering the bioactive components of Chinese herbal medicines.

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“…Other organisms also produce protease including the mushrooms Flammulina velutipes (Arima et al, 2000), Grifola frondosa (Okamura et al, 1997a) and Pleurotus eryngii (Arima et al, 2000), which are popular in Japan. These are also rich in fiber such as glucan, protein and a precursor of vitamin D, in addition to having a preventive effect on thrombosis and cancer (Whistler et al, 1976;Hirasawa et al, 1997;Okamura et al, 1997b;Okamura et al, 2000). In this study we produced a fermented soybean with mushroom mycelia because we hypothesized it might have a preventive effect against thrombosis and cancer, as well as other health benefits.…”

mentioning

confidence: 99%

Fermented Soybean with Thrombosis Preventing Activity Using Mushroom Mycelia as Microbial Source

Okamura-Matsui

Izuta

Tomoda

et al. 2003

FSTR

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Bacillus natto is the main microorganism used to make natto (fermented soybeans), because this microbe has good ability to produce protease. However, it is known that some genera of mushroom also produce protease, and in this study we made a fermented soybean using a mushroom mycelia in place of B. natto. We found that the fermented soybean made using mycelia of Flammulina velutipes and Roseofomes subflexibilis showed a thrombosis preventing activity: a prolonged thrombin clotting time 8.2 fold that of control.

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Fumaric Acid, Anti Thrombin Substance from Rhizopus javanicus.

Cited by 7 publications

References 5 publications

Fumaric acid, an antibacterial component of Aloe vera L.

Fumaric acid, an antibacterial component of Aloe vera L.

Combining ultra‐high‐performance liquid chromatography quadruple exactive orbitrap mass spectrometry with chemometrics to identify and verify the blood‐activating components of hawthorn

Fermented Soybean with Thrombosis Preventing Activity Using Mushroom Mycelia as Microbial Source

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