Fully motorized optical-resolution photoacoustic microscopy

Li, Lei; Yeh, Cheng-Hung; Hu, Song; Wang, Lidai; Soetikno, Brian T.; Chen, Ruimin; Zhou, Qifa; Shung, K. Kirk; Maslov, Konstantin; Wang, Lihong V.

doi:10.1364/ol.39.002117

Cited by 67 publications

(37 citation statements)

References 19 publications

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“…= 100.0 mm, LA1207-A, Thorlabs) corrects for water immersion, and a ring transducer (35 MHz center frequency, 25 MHz bandwidth) detects PA signals. The lateral resolution of the system is limited by the effective numerical aperture of the optical system and has been experimentally quantified as 2.6 µm [18]. …”

Section: Experimental Setup and Methodsmentioning

confidence: 99%

Multiview optical resolution photoacoustic microscopy

Zhu¹,

Li²,

Gao³

et al. 2014

Optica

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Optical resolution photoacoustic microscopy (OR-PAM), while providing high lateral resolution, has been limited by its relatively poor acoustically determined axial resolution. Although this limitation has been tackled in recent works by using either broadband acoustic detection or nonlinear photoacoustic effects, a flexible solution with three dimensional optical resolution in reflection mode remains desired. Herein we present a multi-view OR-PAM technique. By imaging the sample from multiple view angles and reconstructing the data using a multi-view deconvolution method, we have experimentally demonstrated an isotropic optical resolution in three dimensions.

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Section: Experimental Setup and Methodsmentioning

confidence: 99%

Multiview optical resolution photoacoustic microscopy

Zhu¹,

Li²,

Gao³

et al. 2014

Optica

Self Cite

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“…For optical resolution-based photoacoustic instruments, focused ultrasound transducers (either singly or in an array) with mechanical motors are used to scan the organic tissue [13]. For acoustic resolution based-photoacoustic instruments, focused light is used to obtain the target information [7].…”

Section: Introductionmentioning

confidence: 99%

Chromatic aberration free reflective mirror-based optical system design for multispectral photoacoustic instruments

Choi

et al. 2019

THC

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BACKGROUND: Current multispectral photoacoustic instruments must use large and separate combinational structures to obtain various biological tissue information for multispectral ranges. OBJECTIVE: The optical aberration generated from the multispectral photoacoustic systems may reduce the image quality of biological tissue because the improper structures for combining light of different wavelength cannot produce good optical ray convergence points. To prevent this, complex combined structures need to be considered at the design level for multispectral photoacoustic systems. METHODS: In place of an optical refracted lens system, reflective mirrors could be designed for optical systems. To verify our proposed idea, we assessed optical distortion performance using red, green, and blue light, and combined optical light sources to compare their chromatic aberration characteristics. RESULTS: The high optical performance is realized regardless of the wavelength for a light source combined with multiple wavelengths, because our optical system was designed with only a reflective surface. CONCLUSIONS: The designed optical system using a reflective mirror can provide multispectral optical sources (such as infrared, visible, and ultraviolet optical lights) with only one light ray path, without any chromatic aberrations.

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“…A concave lens usually diverges a beam in order to increase the field of the light, and the light is directly transmitted to a target such that a transducer has to be located perpendicularly to the target [16]. A conical lens can twist and focus a light beam to a certain location such that a transducer has to be located in the same line [17,18].…”

Section: Introductionmentioning

confidence: 99%

Development of a Double-Gauss Lens Based Setup for Optoacoustic Applications

Choi

Ryu

Yeom

2017

Sensors

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In optoacoustic (photoacoustic) systems, different echo signal intensities such as amplitudes, center frequencies, and bandwidths need to be compensated by utilizing variable gain or time-gain compensation amplifiers. However, such electronic components can increase system complexities and signal noise levels. In this paper, we introduce a double-Gauss lens to generate a large field of view with uniform light intensity due to the low chromatic aberrations of the lens, thus obtaining uniform echo signal intensities across the field of view of the optoacoustic system. In order to validate the uniformity of the echo signal intensities in the system, an in-house transducer was placed at various positions above a tissue sample and echo signals were measured and compared with each other. The custom designed double-Gauss lens demonstrated negligible light intensity variation (±1.5%) across the illumination field of view (~2 cm diameter). When the transducer was used to measure echo signal from an eye of a bigeye tuna within a range of ±1 cm, the peak-to-peak amplitude, center frequency, and their −6 dB bandwidth variations were less than 2 mV, 1 MHz, and 6%, respectively. The custom designed double-Gauss lens can provide uniform light beam across a wide area while generating insignificant echo signal variations, and thus can lower the burden of the receiving electronics or signal processing in the optoacoustic system.

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Fully motorized optical-resolution photoacoustic microscopy

Cited by 67 publications

References 19 publications

Multiview optical resolution photoacoustic microscopy

Multiview optical resolution photoacoustic microscopy

Chromatic aberration free reflective mirror-based optical system design for multispectral photoacoustic instruments

Development of a Double-Gauss Lens Based Setup for Optoacoustic Applications

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