Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile

Zuccotti, Maurizio; Bellone, Michele; Longo, Frank J.; Redi, Carlo Alberto; Garagna, Silvia

doi:10.1007/s10815-011-9562-4

Cited by 18 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the zygote also inherits from the egg other epigenetic mechanisms regulating gene expression, the best known of which are DNA methylation, histone modifications, and chromatin organization [42]. According to Zuccotti et al [43], maintenance of a favorable transcriptional epigenetic context in the heterochromatin of transcriptionally silent SN oocytes might be related to the early stages of development when transcripts from these heterochromatic regions are functional to preimplantation progression.…”

Section: Discussionmentioning

confidence: 99%

Restraint Stress on Female Mice Diminishes the Developmental Potential of Oocytes: Roles of Chromatin Configuration and Histone Modification in Germinal Vesicle Stage Oocytes1

Yuan

et al. 2015

Biology of Reproduction

View full text Add to dashboard Cite

The mechanisms by which restraint stress impairs oocyte developmental potential are unclear. Factors causing differences between the developmental potential of oocytes with surrounded nucleolus (SN) and that of oocytes with nonsurrounded nucleolus (NSN) are not fully characterized. Furthermore, the relationship between increased histone acetylation and methylation and the increased developmental competence in SN oocytes is particularly worth exploring using a system where the SN configuration can be uncoupled (dissociated) from increased histone modifications. In this study, female mice were subjected to restraint for 24 or 48 h or for 23 days before being examined for oocyte chromatin configuration, histone modification, and development in vitro and in vivo. Results showed that restraint for 48 h or 23 days impaired NSN-to-SN transition, histone acetylation and methylation in SN oocytes, and oocyte developmental potential. However, whereas the percentage of stressed SN oocytes returned to normal after a 48-h postrestraint recovery, neither histone acetylation/methylation in SN oocytes nor developmental competence recovered following postrestraint recovery with equine chorionic gonadotropin (eCG) injection. Priming unstressed mice with eCG expedited oocyte histone modification to an early completion. Contrary to the levels of acetylated and methylated histones, the level of phosphorylated H3S10 increased significantly in the stressed SN oocytes. Together, the results suggest that 1) restraint stress impaired oocyte potential with disturbed histone modifications; 2) SN configuration was uncoupled from increased histone acetylation/methylation in the restraint-stressed oocytes; and 3) the developmental potential of SN oocytes is more closely correlated with epigenetic histone modification than with chromatin configuration.

show abstract

Section: Discussionmentioning

confidence: 99%

Restraint Stress on Female Mice Diminishes the Developmental Potential of Oocytes: Roles of Chromatin Configuration and Histone Modification in Germinal Vesicle Stage Oocytes1

Yuan

et al. 2015

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…Developing oocytes and early-stage embryos are transcriptionally quiescent in mouse, zebrafish, and C. elegans (Su et al, 2007; Xia et al, 2012; Zuccotti et al, 2011); thus post-transcriptional and translational regulation of gene expression is crucial for oocyte development and the oocyte-to-embryo transition. Both the RAS-ERK pathway and Dicer have been shown to regulate key steps in oogenesis.…”

Section: Introductionmentioning

confidence: 99%

A Requirement for ERK-Dependent Dicer Phosphorylation in Coordinating Oocyte-to-Embryo Transition in C. elegans

et al. 2014

View full text Add to dashboard Cite

Signaling pathways and small RNAs direct diverse cellular events, but few examples are known of defined signaling pathways directly regulating small RNA biogenesis. We show that ERK phosphorylates Dicer on two conserved residues in its RNAse IIIb and dsRNA-binding domain, and phosphorylation of these residues is necessary and sufficient to trigger Dicer’s nuclear translocation in worms, mice, and human cells. Phosphorylation of Dicer on either site inhibits Dicer function in the female germ line and dampens small RNA repertoire. Our data demonstrate that ERK phosphorylates and inhibits Dicer during meiosis I for oogenesis to proceed normally in C. elegans and that this inhibition is released before fertilization for embryogenesis to proceed normally. The conserved Dicer residues, their phosphorylation by ERK, and the consequences of the resulting modifications implicate an ERK-Dicer nexus as a fundamental component of the oocyte-to-embryo transition and an underlying mechanism coupling extracellular cues to small RNA production.

show abstract

“…However, in contrast to somatic nucleoli, active ribosomal genes have been described only at the NLB surface (Bouniol-Baly et al, 1999;Pesty et al, 2007). SN-oocytes have higher meiotic and developmental competence than NSN-oocytes and correspond to a more advanced stage of oocyte development (Zuccotti et al, 2002(Zuccotti et al, , 2011Inoue et al, 2008). Recent analysis of the global transcriptome profile in mouse NSN-and SN-oocytes showed that they are very similar but not identical: NSN-oocytes are enriched in methylated and acetylated peptides (Monti et al, 2013), whereas SN-oocytes are characterized by higher levels of methylation and acetylation of DNAs (Kageyama et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA

Shishova

Lavrentyeva

Dobrucki

et al. 2015

Developmental Biology

View full text Add to dashboard Cite

It is well known that fully-grown mammalian oocytes, rather than typical nucleoli, contain prominent but structurally homogenous bodies called "nucleolus-like bodies" (NLBs). NLBs accumulate a vast amount of material, but their biochemical composition and functions remain uncertain. To clarify the composition of the NLB material in mouse GV oocytes, we devised an assay to detect internal oocyte proteins with fluorescein-5-isothiocyanate (FITC) and applied the fluorescent RNA-binding dye acridine orange to examine whether NLBs contain RNA. Our results unequivocally show that, similarly to typical nucleoli, proteins and RNA are major constituents of transcriptionally active (or non-surrounded) NLBs as well as of transcriptionally silent (or surrounded) NLBs. We also show, by exposing fixed oocytes to a mild proteinase K treatment, that the NLB mass in oocytes of both types contains nucleolar proteins that are involved in all major steps of ribosome biogenesis, including rDNA transcription (UBF), early rRNA processing (fibrillarin), and late rRNA processing (NPM1/nucleophosmin/B23, nucleolin/C23), but none of the nuclear proteins tested, including SC35, NOBOX, topoisomerase II beta, HP1α, and H3. The ribosomal RPL26 protein was detected within the NLBs of NSN-type oocytes but is virtually absent from NLBs of SN-type oocytes. Taking into account that the major class of nucleolar RNA is ribosomal RNA (rRNA), we applied fluorescence in situ hybridization with oligonucleotide probes targeting 18S and 28S rRNAs. The results show that, in contrast to active nucleoli, NLBs of fully-grown oocytes are impoverished for the rRNAs, which is consistent with the absence of transcribed ribosomal genes in the NLB mass. Overall, the results of this study suggest that NLBs of fully-grown mammalian oocytes serve for storing major nucleolar proteins but not rRNA.

show abstract

Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile

Cited by 18 publications

References 29 publications

Restraint Stress on Female Mice Diminishes the Developmental Potential of Oocytes: Roles of Chromatin Configuration and Histone Modification in Germinal Vesicle Stage Oocytes1

Restraint Stress on Female Mice Diminishes the Developmental Potential of Oocytes: Roles of Chromatin Configuration and Histone Modification in Germinal Vesicle Stage Oocytes1

A Requirement for ERK-Dependent Dicer Phosphorylation in Coordinating Oocyte-to-Embryo Transition in C. elegans

Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA

Contact Info

Product

Resources

About