Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli

Rüffer, N.; Heidersdorf, U.; Kretzers, I.; Sprenger, Georg A.; Raeven, Leon; Takors, Ralf

doi:10.1007/s00449-004-0354-4

Cited by 57 publications

(29 citation statements)

References 33 publications

(36 reference statements)

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“…The fermentation broth is directed through a bypass crossflow, hollow-fiber ultrafiltration unit and recirculated into the bioreactor (Rüffer et al 2004). …”

Section: Ultrafiltrationmentioning

confidence: 99%

Recovery of succinic acid from fermentation broth

2009

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Succinic acid is of high interest as bio-feedstock for the chemical industry. It is a precursor for a variety of many other chemicals, e.g. 1,4-butandiol, tetrahydrofuran, biodegradable polymers and fumaric acid. Besides optimized production strains and fermentation processes it is indispensable to develop cost-saving and energy-effective downstream processes to compete with the current petrochemical production process. Various methods such as precipitation, sorption and ion exchange, electrodialysis, and liquid-liquid extraction have been investigated for the recovery of succinic acid from fermentation broth and are reviewed critically here.

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“…The fermentation broth is directed through a bypass crossflow, hollow-fiber ultrafiltration unit and recirculated into the bioreactor (Rüffer et al 2004). …”

Section: Ultrafiltrationmentioning

confidence: 99%

Recovery of succinic acid from fermentation broth

2009

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“…Hierbei wurden ein Scale-up sowie eine theoretische Prozessoptimierung simuliert. Durch die reaktionsintegrierte Extraktion konnte die Ausbeute erhöht und die Nebenproduktbildung um 28 % reduziert werden [36], jedoch liegen keine Extraktionsdaten zu Zwischenprodukten im Syntheseweg vor. Darüber hinaus gibt es theoretische Arbeiten zur reaktionsintegrierten Adsorption und Chromatographie bei monoenzymatischen Gleichgewichtsreaktionen [17], welche jedoch auch keine Co-Adsorption von Zwischenprodukten in einem Mehrenzymsystem berücksichtigen.…”

Section: Prozesssimulationunclassified

Simulation of Reaction Integrated Adsorption of Trienzymatic Synthesized Laminaribiose

Waluga

Zein

Jördening

et al. 2013

Chemie Ingenieur Technik

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Simulation of Reaction Integrated Adsorption of Trienzymatic Synthesized LaminaribioseA simulation for the reaction integrated adsorption of trienzymatic synthesized laminaribiose was created, based on three individual enzymatic kinetics and equilibrium data from pure substance measurements. A discontinuous integration of reaction and adsorption was able to increase the yield by one third. A continuous adsorption with a cyclic transfer of loaded and replacement with fresh adsorbent can increase the yield by even 2.4 times. However, if the replacement cycle of the adsorbent is too short, the discharge of intermediates results in a reduced product formation.

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“…The production phase of the strain E. coli 4pF81 (see Rüffer et al, 2004 for a detailed description) was studied using the sensor reactor approach as described in (El Massaoudi et al, 2003). With this approach, a series of 13 C labeling experiments can be performed under production like conditions.…”

Section: Application To a Series Of Mfa Experiments Metabolic Modelmentioning

confidence: 99%

Interpretation of metabolic flux maps by limitation potentials and constrained limitation sensitivities

Wahl

Wiechert

2006

Biotech & Bioengineering

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Two new concepts, "Limitation Potential" and "Constraint Limitation Sensitivity" are introduced that use definitions derived from metabolic flux analysis (MFA) and metabolic network analysis (MNA). They are applied to interpret a measured flux distribution in the context of all possible flux distributions and thus combine MFA with MNA. The proposed measures are used to quantify and compare the influence of intracellular fluxes on the production yield. The methods are purely based on the stoichiometry of the network and constraints that are given from irreversible fluxes. In contrast to metabolic control analysis (MCA), within this approach no information about the kinetic mechanisms are needed. A limitation potential (LP) is defined as the reduction of the reachable (theoretical) maximum by a measured flux. Measured fluxes that strongly narrow the reachable maximum are assumed to be limiting as the network has no ability to counterbalance the restriction due to the observed flux. In a second step, the sensitivity of the reduced maximum is regarded. This measure provides information about the necessitated changes to reach higher yields. The methods are applied to interpret the capabilities of a network based on measured fluxes for a L-phenylalanine producer. The strain was examined by a series of experiments and three flux maps of the production phase are analyzed. It can be shown that the reachable yield is drastically reduced by the measured efflux into the TCA cycle, while the oxidative pentose-phosphate pathway only plays a secondary role on the reachable maximum.

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Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli

Cited by 57 publications

References 33 publications

Recovery of succinic acid from fermentation broth

Recovery of succinic acid from fermentation broth

Simulation of Reaction Integrated Adsorption of Trienzymatic Synthesized Laminaribiose

Interpretation of metabolic flux maps by limitation potentials and constrained limitation sensitivities

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