Fully Enzymatic Inulin Determination in Small Volume Samples without Deproteinization

Abstract

A fully enzymatic method for the determination of inulin in serum or plasma without deproteinization is described. The assay is carried out by means of a fructose determination after hydrolysis of inulin via inulinase and simultaneous oxidation of the native glucose using glucose oxidase and H₂O₂. This time-saving procedure of high specificy, sensitivity and accuracy requires only small sample volumes. The usefulness of the method is shown by analysis of blood levels in humans and rabbits after an intravenous administration of Inutest®.

Determination of the Glomerular Filtration Rate by Identification of Sinistrin Kinetics

“…The quantification is carried out after the removal of native glucose by enzymatic oxidation grade II glucose oxidase 1 ) exhibiting some catalase activity in combination with hydrogen peroxide (18). Both the glucose oxidation and the hydrolysis 2 ) of sinistrin are performed at 56 °C within 15 minutes.…”

Mucorales isolados do solo de mineração de cobre e produção de amilase e inulinase

“…Dentre as enzimas de interesse industrial, a inulinase (2,1-β-D-fructanohidrolase EC 3.2.1.7) hidrolisa a inulina proporcionando um xarope rico em frutose (95%), de grande importância na indústria alimentícia, por possuir maior poder adoçante, menores cariogenicidade e teor calórico (Bucke 1981;Vandame & Derycke 1983;Bajpai & Margaritis 1985;Fuchs et al 1985). Na área médica, a inulinase é utilizada como ferramenta de diagnóstico em problemas renais (Kuehnle et al 1992). Por outro lado, as amilases catalisam a hidrólise do amido e podem ser encontradas em plantas, animais e microrganismos, dentre esses, os fungos, sendo utilizadas na fabricação de medicamentos, papel, tecido, pão e na liquefação do amido (Lowe 1992 …”

How Measuring Glomerular Filtration Rate? Comparison of Reference Methods

“…Moreover, most of the methods (except the enzymatic ones) are prone to interferences with glucose measurement which is a limiting factor when measuring GFR in diabetic patients (Little, 1949). Regarding the methods for measuring inulin, we can cite the "acid" methods (Kuehnle et al, 1992;Shaffer & Somogoyi, 1933;Alving et al, 1939;Corcoran, 1952;Rolf et al, 1949;Roe, 1934;Steinitz, 1938;Hubbard & Loomis, 1942;Lentjes et al, 1994;Heyrovsky, 1956;Rolf et al, 1949), the enzymatic methods (Day & Workman, 1984;Delanghe et al, 1991;Jung et al, 1990;Summerfield et al, 1993;Dubourg et al, 2010) and the new methods by high performance liquid chromatography (HPLC) (Ruo et al, 1991;Baccard et al, 1999;Dall'Amico et al, 1995;Pastore et al, 2001). Describing these methods in detail are beyond the scope of this chapter and we propose the readers the following reference if they are interested in this topic (Delanaye et al, 2011b).…”