Fully biodegradable and biocompatible emulsion templated polymer scaffolds by thiol-acrylate polymerization of polycaprolactone macromonomers

Abstract

(2015) 'Fully biodegradable and biocompatible emulsion template polymer scaolds by thiol-acrylate polymerisation of polycaprolactone macropolymers.', Polymer chemistry., 6 (41). pp. 7256-7263. Further information on publisher's website:http://dx.doi.org/10.1039/C5PY00721FPublisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. The emulsion templating process offers a route to highly porous polymers with well--defined morphologies. This study describes the preparation of such porous polymers (polyHIPEs) via the photopolymerization of a multi--functional thiol and polycaprolactone macromonomer. The resulting materials have nominal porosities of 90% and 95%, and are seen to have an interconnected pore morphology, with an average pore diameter of approximately 60 µm. Initial biocompatibility assessments with fibroblast cells (L929) have shown that the polymers are capable of supporting cell growth over 7 days and degradation products are non--toxic to cells up to a concentration of 0.1 mg ml --1 .

Optimized peptide functionalization of thiol‐acrylate emulsion‐templated porous polymers leads to expansion of human pluripotent stem cells in 3D culture

“…Ethanol concentrations used were 30, 50, 70, 80, 90, 95, and finally 100%. Samples were cleared in Histoclear, and embedded in paraffin as previously described by Johnson et al 32 Sectioning was completed using a Cut4060 Microtome (microTec) prior to floating on a 40 C histology water bath and mounting onto coated poly-L-lysine slides. Scaffolds were stained for visualization of the cells using Hematoxylin and Eosin (H&E) staining.…”

“…A polystyrene‐based polyHIPE scaffold is commercially available for use in three‐dimensional cell culture under the tradename Alvetex® . A range of other polyHIPE compositions are being investigated as scaffolds for specific cell and tissue types, including neurons, chondrocytes, muscle stem cells, fibroblasts, and osteoblasts …”

The Chemistry of Porous Polymers: The Holey Grail

“…Polymerization chemistries are being developed to take advantage of monomers from renewable resources and macromolecular structural chemistries are being developed to enhance degradability. PHs that were based on renewable resource monomers such as polyphenols (e. g., tannin, tannic acid, lignin), plant oils (e. g., soybean, castor), polysaccharides (e. g., alginate, chitosan, dextrin, pectin), and lactide have recently been developed, as were PHs that were based on polymers containing degradable groups (e. g., esters) . In addition, the recent advent of reactive surfactants and/or reactive nanoparticles for HIPE stabilization has produced a significant reduction in the amounts of leachable components.…”

Hybrid Nanomaterials by Surface Grafting of Synthetic Polypeptides Using N‐Carboxyanhydride (NCA) Polymerization

“…[44] Poly(HIPE)s possess high interconnected porosity making them useful for the applications in tissue engineering, 3D cell culture, filtration/separation techniques, etc. [45,46] PLG and poly(L-lysine) (PLL) formed high density brushes rendering the material pH responsive. Furthermore, the availability of these newly introduced functional group for further applications such as bioconjugation was demonstrated by conjugation of enhanced green fluorescent protein ( Figure 1B).…”