Fully Automated Three‐Dimensional Column‐Switching SPE–FIA–HPLC System for the Characterization of Lipids by a Single Injection: Part I. Instrumental Design and Chemometric Approach to Assess the Effect of Experimental Settings on the Response of ELSD

Arslan, Fatma Nur; Kara, Hüseyin

doi:10.1007/s11746-015-2750-0

Cited by 6 publications

(12 citation statements)

References 29 publications

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“…The tocol (tocopherol and tocotrienol) profiles of the PSOs were identified using a normal-phase liquid chromatographic (NP-HPLC) method (Arslan et al, 2016). An Agilent 1200 series HPLC system (Agilent Technologies Inc., USA) was equipped with a G1321C model fluorescence detector (FLD) and G1316A model column compartment set at 20 °C.…”

Section: Determination Of the Tocol Profile By Hplc-fldmentioning

confidence: 99%

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

et al. 2018

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SUMMARY:There is a growing interest in cold-pressed oils because they present high contents of bioactive compounds. These oils have the characteristic properties of seeds and are specific products of their regions. The aim of this study was to determine the compositions and contents of fatty acids, phytosterols, squalene, tocols, phenolic acids, carotenoids and phenolic bioactives, and the free radical scavenging as well as antioxidant activities of coldpressed pumpkin (Cucurbita pepo L.) seed oils. Oil samples from raw pumpkin seeds cultivated in four different central Anatolia regions of Turkey were prepared using a laboratory screw-pressing machine. The results indicate that coldpressed pumpkin seed oils (PSO) have excellent quality with high contents of polyunsaturated fatty acids (%∑PUFAs) (53.60 ±0.06−53.73 ±0.05), total phytosterols (782.1±9.7-805.2 ±11.3 mg/100 g oil), squalene (591.3±10.6-632.5±11.4 mg/100 g oil), tocols (97.79 ±0.76−94.29 ±0.34 mg/100 g oil), phenolic acids (22.73 ±0.41-23.98 ±0.46 mg/100 g oil), carotenoids (6.95 ±0.03-7.60 ±0.03 mg/100 g oil), total phenolics (3.96 ±0.13 -5.82±0.15 mg GAE/100 g), free radicalscavenging activity (5.70 ±0.13−7.35 ±0.15 mg GAE/100 g) and total antioxidant activity (26.67±0.97−38.89±1.41 mg GAE/100 g) values. Thus, this study demonstrates that the cold-pressed PSOs from the central Anatolia regions of Turkey are an excellent source of natural bioactive compounds, free of chemical contaminants and nutritious.KEYWORDS: Antioxidant activity; Bioactive compound; Cold-pressed oil; Cucurbita pepo L.; Phenolic acid; Tocol profile RESUMEN: Aceites de semillas de calabaza prensados en frío (Cucurbita pepo L.) de la región central de Anatolia en Turquía: Caracterización de fitosteroles, escualeno, tocoles, ácidos fenólicos, carotenoides y compuestos bioactivos de ácidos grasos. Existe un interés creciente en los aceites prensados en frío, ya que presentan altos contenidos de compuestos bioactivos. Estos aceites tienen las propiedades características de las semillas y son productos específicos de las regiones. El objetivo de este estudio fue determinar las composiciones y contenidos de ácidos grasos, fitoesteroles, escualeno, tocoles, ácidos fenólicos, carotenoides y bioactivos fenólicos, la actividad de eliminación de radicales libres, así como las actividades antioxidantes de aceites de semillas de calabaza prensados en frío (Cucurbita pepo L.). Las muestras de aceite de semillas de calabaza cultivadas en cuatro regiones diferentes de Anatolia central de Turquía se prepararon utilizando una máquina de laboratorio de prensado mediante tornillo. Los resultados indican que los aceites de semilla de calabaza prensados en frío (PSO) tienen una excelente calidad, con altos contenidos de ácidos grasos poliinsaturados (%ΣPUFA) (53,60±0,06-53,73±0,05), fitoesteroles totales (782,1±9,7-805,2±11,3 mg/100 g de aceite), escualeno (591,3 ± 10,6-632,5 ± 11,4 mg/100 g de aceite), tocols (97,79±0,76-94,29±0,34 mg/100 g de aceite), ácidos fenólicos (22,73 ± 0,41-23,98 ± 0.46 mg/10...

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Section: Determination Of the Tocol Profile By Hplc-fldmentioning

confidence: 99%

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

et al. 2018

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“…[5,6,8,9] Several factors affecting ELSD detection response include evaporator and temperature, nebulizing gas flow rate, also mobile phase composition and flow rate. [4,8,10] These factors need to be optimized to achieve maximum detector response for better sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Evaporative Light Scattering Detector using Response Surface Methodology for Liquid Chromatography Analysis of Frondoside A

Tasfiyati¹,

Prasetia²,

Muzdalifah³

et al. 2022

ChemistrySelect

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Response surface methodology is employed to investigate the optimum evaporative light scattering detector (ELSD) operating conditions for high performance liquid chromatography (HPLC) analysis of frondoside A, which is a bioactive compound valuable for its anti-cancer activity. The results suggest that ELSD response is mostly affected by nebulizing gas flow rate and evaporator temperature. The optimum response of frondoside A is achieved by adjusting ELSD evaporator and nebulizer temperature at 80 °C and 35 °C, respectively, with nebulizing gas flow rate of 0.9 SLM. The analysis of variance (ANOVA) test results demonstrated that the established model can successfully describe the relationship between the signal response and ELSD experimental parameters, and predict the optimum ELSD parameters for frondoside A analysis. Furthermore, analytical method validation performed in optimum conditions shows that the proposed method also exhibits adequate sensitivity and linearity, with acceptable precision and accuracy. The results demonstrate the suitability of HPLC-ELSD technique for the development of routine analysis procedures of frondoside A in natural product samples.

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“…Among these novel reversed-phases, the C 30 stationary phases offer greater shape selectivity in the resolution of tocols compared to conventional reversed-phase columns. Their superior performance results from their rigid, highly ordered longer C 30 alkyl groups and dense surface coverage [18,27], Although reversed-phase HPLC methods using C 30 phases have already been described for the determination of tocols, their performance was not ideal due to the need for additional sample pre-treatment, use of large volumes or exotic eluents and long analysis times [11,18,22,26], Hence, it is necessary to optimize the experimental parameters that affect the separation performance of C 30 stationary phase for the close-eluting β-and γ-tocol peaks.…”

Section: Introductionmentioning

confidence: 99%

“…In most method development studies, analysts have traditionally followed the practice of altering "one-variable-ata-time" or a "step-by-step" optimization. These approaches for method optimization are laborious, time consuming, and unsuccessful for detecting the accurate optimum conditions as they do not take into account interactions among the experimental factors [28][29][30]. Multi-factor experimental designs, such as the response surface methodology (RSM), are widely used for method optimization since they are less laborious and faster as they allow considering mutually influencing experimental factors in a rapid manner.…”

Section: Introductionmentioning

confidence: 99%

Chemometrics-Assisted Optimization of Beta-/Gamma-Tocol Separation on a C30 Stationary Phase in Reversed-Phase LC

Arslan

Janssen

2018

Chromatographia

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This paper presents a chemometrics-assisted optimization study to improve the separation of tocopherol (-T) and tocotrienol (-TT) homologues on a C 30 stationary phase in reversed-phase HPLC. The HPLC settings were optimized using a central composite design and the response surface methodology. Flow rate, column temperature, and mobile phase composition were chosen as independent variables. Peak resolution (R s), analysis time (t R), and peak symmetries of the tocopherol isomers were chosen as response variables. Optimum performance in terms of R s was obtained at a flow rate of 0.31 mL min −1 , a temperature of 8.70 °C, and % B content (methyl tert-butyl ether: methanol: water, 80:18:2, v/v/v) in the mobile phase of 38.12%. The analysis of variance and regression analysis gave adjusted R 2 values of 0.9841 for R s , 0.9850 for t R-(α-T), 0.9853 for t R-(β-T), and 0.9204 for the peak symmetry of β-T. This confirms the good agreement of experimental data with predicted values. The close eluting peaks of β-/γ-tocol could be baseline separated at the optimized conditions at a minimized analysis time. Empirical second-order polynomial models were derived that gave statistically high significances (P < 0.0001). Hence, the models can be successfully employed to predict the optimum separation conditions of co-eluting peaks of β-/γ-tocols. The optimized method was successfully applied to determine the individual tocol homologues in various cold pressed edible oils. Total contents ranged from 15 to almost 2600 mg tocol kg −1 oil.

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Fully Automated Three‐Dimensional Column‐Switching SPE–FIA–HPLC System for the Characterization of Lipids by a Single Injection: Part I. Instrumental Design and Chemometric Approach to Assess the Effect of Experimental Settings on the Response of ELSD

Cited by 6 publications

References 29 publications

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

Cold-pressed pumpkin seed (<em>Cucurbita pepo</em> L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

Optimization of Evaporative Light Scattering Detector using Response Surface Methodology for Liquid Chromatography Analysis of Frondoside A

Chemometrics-Assisted Optimization of Beta-/Gamma-Tocol Separation on a C30 Stationary Phase in Reversed-Phase LC

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