Fully automated, high speed, tomographic phase object reconstruction using the transport of intensity equation in transmission and reflection configurations

Nguyen, Thanh; Nehmetallah, George; Tran, Dat Q.; Darudi, Ahmad; Soltani, Peyman

doi:10.1364/ao.54.010443

Cited by 27 publications

(24 citation statements)

References 16 publications

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“…It should be also noted that with short camera exposure time (50 ms), the current imaging speed (1 frame/s) is ultimately limited by the z-scanning mechanics. There is still plenty of room for speed improvement (to video rate or even camera frame-rate limited speed), if a spatial light modulator 26,34 or an electrically tunable lenses 33,93 is used to replace the scanning mechanics.…”

Section: Multi-modal Computational Imaging Of Hela Cell Apoptosismentioning

confidence: 99%

High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

Zuo

Sun

et al. 2017

Sci Rep

286

138

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For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with circular illumination aperture, partial coherence tends to diminish the phase contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE imaging, resulting in strong low-frequency artifacts and compromised imaging resolution. Here, we demonstrate how these issues can be effectively addressed by replacing the conventional circular illumination aperture with an annular one. The matched annular illumination not only strongly boosts the phase contrast for low spatial frequencies, but significantly improves the practical imaging resolution to near the incoherent diffraction limit. By incorporating high-numerical aperture (NA) illumination as well as high-NA objective, it is shown, for the first time, that TIE phase imaging can achieve a transverse resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse imaging of in vitro Hela cells revealing cellular morphology and subcellular dynamics during cells mitosis and apoptosis is exemplified. Given its capability for high-resolution QPI as well as the compatibility with widely available brightfield microscopy hardware, the proposed approach is expected to be adopted by the wider biology and medicine community.

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Section: Multi-modal Computational Imaging Of Hela Cell Apoptosismentioning

confidence: 99%

High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

Zuo

Sun

et al. 2017

Sci Rep

286

138

View full text Add to dashboard Cite

show abstract

“…There remains a huge amount of work to be accomplished in both improving the inversion algorithms that are required to invert the data and reconstruct the image and for optimizing the experimental configurations used for ptychography [18][19][20]. In a previous work, an electrically tunable liquid crystal lens (ETL) with a variable focal length was employed in the TIE system to mimic diffraction and serves to replace the mechanical translation of the CCD camera [14,24]. This system has many advantages since it resulted in faster acquisition of the intensity patterns and it is immune to translational misalignment noise of the CCD camera making it more suitable to record dynamical events.…”

Section: Introductionmentioning

confidence: 99%

“…Also, the derivative estimation was improved because of the ability of the system to capture multiple intensity images at different planes in a short period of time, thus reducing the error in the derivative estimation. Thus, one can construct a fully automated 360-degree field of view 4f tomographic TIE system using a tunable lens [24,25]. Although the ETL-TIE system is superior in acquisition time and more immune to noise, the speed of the overall system is controlled by the speed of the electrically tunable lens.…”

Section: Introductionmentioning

confidence: 99%

“…A relation between the SLM pattern focal length and axial translation is derived. A detailed analysis of the 3D tomographic reconstruction algorithm using the Fourier Slice theorem on TIE obtained simulated phase images is also developed [4,24,27,28]. Using the Fourier Slice theorem applied to weakly scattering objects (a small refractive index span that assumes that light propagates along straight lines within the sample) immersed in matching index liquid, experimental tomographic reconstruction results are also obtained.…”

Section: Introductionmentioning

confidence: 99%

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Non-Interferometric Tomography of Phase Objects Using Spatial Light Modulators

Nguyen

Nehmetallah

2016

J. Imaging

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Quantitative 3D phase retrieval techniques are based on either interferometric techniques such as holography or noninterferometric intensity-based techniques such as the transport of intensity equation (TIE). Interferometric techniques are vibration-sensitive and often use a reference beam requiring complicated optical alignment. In this work we develop a simple, fast, and noninterferometric tomographic 3D phase retrieval technique based on the TIE which does not suffer from such drawbacks. The optical setup is a modified 4f TIE system which uses an SLM to replace the slow translation of the CCD required to record several diffraction patterns in a traditional TIE system. This novel TIE setup is suitable for dynamical events such as imaging biological processes. A rotating mechanical stage is constructed to obtain tomographic phase images of the object. The tomographic reconstruction algorithm is based on the Fourier slice theorem (backprojection algorithm) which applies to objects with a small refractive index span. Simulation and experimental results are shown as part of this work. A graphical user interface is developed to perform the TIE tomographic reconstruction algorithm and to synchronize the captured intensities by the CCD, the phase patterns displayed on the SLM, and the Arduino controlled rotating stage assembly.

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“…For solving the TIE, many effective numerical methods such as the method based on Green's function [19], multigrid (MG) [20,21], and the Zernike polynomial expansion method [22,23] were presented. Currently, TIE technique is used in different fields of physics such as adaptive optics [24,25], microscopy [26,27], optical measuring [28][29][30], and optical tomography [31][32][33], just to name a few.…”

Section: Introductionmentioning

confidence: 99%

A guide to properly select the defocusing distance for accurate solution of transport of intensity equation while testing aspheric surfaces

et al. 2016

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Fully automated, high speed, tomographic phase object reconstruction using the transport of intensity equation in transmission and reflection configurations

Cited by 27 publications

References 16 publications

High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

Non-Interferometric Tomography of Phase Objects Using Spatial Light Modulators

A guide to properly select the defocusing distance for accurate solution of transport of intensity equation while testing aspheric surfaces

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