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Tef is an indigenous and important food, feed and cash crop to the small-holder Ethiopian farmers. Knowledge of the natural genetic composition of the crop provides the option to further exploit its genetic potential through breeding. However, there are insu cient reports on the genetic variability of Ethiopian tef using a medium-throughput marker system. Hence, the current study was designed to evaluate the genetic variability of released and core germplasm which were collected earlier. Eighty-one tef genotypes collected from eight Ethiopian ecological zones and release variety were targeted using 14 SSR markers. The study ensued a total of 122.43 alleles through the entire loci and populations. All the used microsatellite loci were highly polymorphic with PIC ranging from 0.96 to 0.98 and mean of 0.976. The analysis showed the presence of high allelic diversity ranging from 0.94 to 0.97 with overall mean of 0.96. The molecular variance analysis indicated the existence of large genetic differentiation (FIS and FIT=0.87) with 86% and 13% of the total variation accounted by among genotypes within population and across from all population respectively. Whereas low genetic differentiation among population (FST=0.014. which accounts 1 %.) was observed. Multivariate analysis like clustering, and PCoA, analysis did not cluster genotypes into distinct groups according to their geographical areas of population. This is due to presumably gene ow among populations. The information of this study may be used as input for breeders in selecting breeding materials.No. Genotypes Origin No. Genotypes Origin DZ
Tef is an indigenous and important food, feed and cash crop to the small-holder Ethiopian farmers. Knowledge of the natural genetic composition of the crop provides the option to further exploit its genetic potential through breeding. However, there are insu cient reports on the genetic variability of Ethiopian tef using a medium-throughput marker system. Hence, the current study was designed to evaluate the genetic variability of released and core germplasm which were collected earlier. Eighty-one tef genotypes collected from eight Ethiopian ecological zones and release variety were targeted using 14 SSR markers. The study ensued a total of 122.43 alleles through the entire loci and populations. All the used microsatellite loci were highly polymorphic with PIC ranging from 0.96 to 0.98 and mean of 0.976. The analysis showed the presence of high allelic diversity ranging from 0.94 to 0.97 with overall mean of 0.96. The molecular variance analysis indicated the existence of large genetic differentiation (FIS and FIT=0.87) with 86% and 13% of the total variation accounted by among genotypes within population and across from all population respectively. Whereas low genetic differentiation among population (FST=0.014. which accounts 1 %.) was observed. Multivariate analysis like clustering, and PCoA, analysis did not cluster genotypes into distinct groups according to their geographical areas of population. This is due to presumably gene ow among populations. The information of this study may be used as input for breeders in selecting breeding materials.No. Genotypes Origin No. Genotypes Origin DZ
The Yellow passion fruit belongs to the Passifloraceae family with great economic, nutritional and social importance in Colombia. It presents a great phenotypic and genotypic diversity, which has not yet been explored or used in genetic improvement programs. The objective of this study was to evaluate the genetic diversity of 84 cultivars of Passiflora edulis f. flavicarpa from nine farms in the municipality of Miraflores, Boyacá, using eight microsatellite markers (SSR). On the basis of this information, estimates of genetic diversity parameters, molecular variance analysis (AMOVA), genetic distances, and cluster of cultivars were obtained. Low levels of genetic differentiation between cultivars were observed in the Bayesian analysis using Structure software, as well as the absence of correlation between genetic and geographic distances. The observed heterozygosity (0.50) was greater than the expected heterozygosity (0.43), suggesting a significant number of heterozygous individuals. The number of alleles per locus varied from 2 to 4, with a mean 2.88. In general, SSR were classified as informative (0.36). The average value of the Shannon Index was 0.71, which shows moderate variability in this cultivar. AMOVA showed higher diversity within cultivars (98%). The gene flow (Nm=28.4) was moderate, this can be explained by the flow of pollen between the different cultivars, the reproduction system of the species, self-incompatibility and the introduction of genotypes from other sites by farmers. The genetic diversity identified in this study is sufficient to initiate breeding programs aimed at identifying cultivars with higher yields.
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