Full-Length Transcriptome Survey and Expression Analysis of Parasitoid Wasp Chouioia cunea upon Exposure to 1-Dodecene

Pan, Lina; Guo, Meiqi; Jin, Xin; Sun, Zhiguo; Jiang, Hao; Han, Jiayi; Wang, Yonghui; Yan, Chuncai; Li, Min

doi:10.1038/s41598-019-54710-0

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…This could be attributed to the limited selection range of candidate compounds, which were exclusively derived from corn and host larvae. In complex natural environments, parasitoid wasps also rely on other chemical signals to locate hosts, such as host feces and host sex pheromones. , Therefore, to gain a comprehensive understanding of the broader olfactory function of CrufCSP3, future studies should explore a wider range of compounds.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Chemosensory Protein CrufCSP3 in Perception of the Host Location in a Parasitic Wasp Cotesia ruficrus

Han,

Wang,

et al. 2024

J. Agric. Food Chem.

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Chemosensory proteins (CSPs) constitute a class of olfactory proteins localized in insect sensory organs that serve a crucial function in decoding external chemical stimuli. This study aims to elucidate the involvement of CrufCSP3 in olfactory perception within the context of Cotesia ruficrus, an indigenous endoparasitoid targeting the invasive pest Spodoptera frugiperda. Through fluorescence-competitive binding assays and site-directed mutagenesis, we pinpointed four amino acids as pivotal residues involved in the interaction between CrufCSP3 and five host-related compounds. Subsequent RNA interference experiments targeting CrufCSP3 unveiled a reduced sensitivity to specific host-related compounds and a decline in the parasitism rate of the FAW larvae. These findings unequivocally indicate the essential role of CrufCSP3 in the chemoreception process of C. ruficrus. Consequently, our study not only sheds light on the functional importance of CSPs in parasitic wasp behavior but also contributes to the development of eco-friendly and efficacious wasp behavior modifiers for effectively mitigating pest population surges.

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Section: Discussionmentioning

confidence: 99%

Involvement of Chemosensory Protein CrufCSP3 in Perception of the Host Location in a Parasitic Wasp Cotesia ruficrus

Han,

Wang,

et al. 2024

J. Agric. Food Chem.

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“…SMRT sequencing can provide a new comprehension of full-length sequences, greatly facilitating the transcriptome research of species lacking high-quality reference genomes and improving genome annotation. To date, only a few full-length transcriptome analyses have been reported in insects, such as Dendroctonus ponderosae ( Keeling et al, 2012 ), Chouioia cunea ( Pan et al, 2019 ), Sogatella furcifera ( Chen et al, 2020 ), Aphis aurantii ( Hong et al, 2020 ), Rhynchophorus ferrugineus ( Yang H et al, 2020 ), Bactrocera dorsalis ( Ouyang et al, 2021 ), Spodoptera frugiperda ( Yang et al, 2021 ), Diabrotica virgifera ( Zhao et al, 2021 ). There are still no published reference genomes for predatory stink bugs.…”

Section: Discussionmentioning

confidence: 99%

A full-length transcriptome and gene expression analysis of three detoxification gene families in a predatory stink bug, Picromerus lewisi

Wang

Jiang

et al. 2022

Front. Physiol.

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The predatory stink bug P. Lewisi shows potential for Integrated Pest Management programs for controlling Lepidoptera pest insects in crops and forests. The importance of this insect for biological control has stimulated several studies into its biology and ecology. However, P. lewisi has little genetic information available. In the present study, PacBio single-molecule real-time (SMRT) sequencing and Illumina RNA-seq sequencing technologies were used to reveal the full-length transcriptome profiling and tissue-specific expression patterns of P. lewisi. A total of 12,997 high-quality transcripts with an average length of 2,292 bp were obtained from different stages of P. lewisi using SMRT sequencing. Among these, 12,101 were successfully annotated in seven public databases. A total of 67 genes of cytochrome P450 monooxygenases, 43 carboxylesterase genes, and 18 glutathione S-transferase genes were identified, most of which were obtained with full-length ORFs. Then, tissue-specific expression patterns of 5th instar nymphs were analyzed using Illumina sequencing. Several candidate genes related to detoxification of insecticides and other xenobiotics as well as the degradation of odors, were identified in the guts and antennae of P. lewisi. The current study offered in-depth knowledge to understand the biology and ecology of this beneficial predator and related species.

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“…AS transcripts were predicted following methods described by Pan et al [ 71 ]. All sequences were run all-vs-all BLAST with high identity settings [ 72 ] against the assembled FL transcriptome.…”

Section: Methodsmentioning

confidence: 99%

Characterization of full-length transcriptome in Saccharum officinarum and molecular insights into tiller development

et al. 2021

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Background Although extensive breeding efforts are ongoing in sugarcane (Saccharum officinarum L.), the average yield is far below the theoretical potential. Tillering is an important component of sugarcane yield, however, the molecular mechanism underlying tiller development is still elusive. The limited genomic data in sugarcane, particularly due to its complex and large genome, has hindered in-depth molecular studies. Results Herein, we generated full-length (FL) transcriptome from developing leaf and tiller bud samples based on PacBio Iso-Seq. In addition, we performed RNA-seq from tiller bud samples at three developmental stages (T0, T1 and T2) to uncover key genes and biological pathways involved in sugarcane tiller development. In total, 30,360 and 20,088 high-quality non-redundant isoforms were identified in leaf and tiller bud samples, respectively, representing 41,109 unique isoforms in sugarcane. Likewise, we identified 1063 and 1037 alternative splicing events identified in leaf and tiller bud samples, respectively. We predicted the presence of coding sequence for 40,343 isoforms, 98% of which was successfully annotated. Comparison with previous FL transcriptomes in sugarcane revealed 2963 unreported isoforms. In addition, we characterized 14,946 SSRs from 11,700 transcripts and 310 lncRNAs. By integrating RNA-seq with the FL transcriptome, 468 and 57 differentially expressed genes (DEG) were identified in T1vsT0 and T2vsT0, respectively. Strong up-regulation of several pyruvate phosphate dikinase and phosphoenolpyruvate carboxylase genes suggests enhanced carbon fixation and protein synthesis to facilitate tiller growth. Similarly, up-regulation of linoleate 9S-lipoxygenase and lipoxygenase genes in the linoleic acid metabolism pathway suggests high synthesis of key oxylipins involved in tiller growth and development. Conclusions Collectively, we have enriched the genomic data available in sugarcane and provided candidate genes for manipulating tiller formation and development, towards productivity enhancement in sugarcane.

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Full-Length Transcriptome Survey and Expression Analysis of Parasitoid Wasp Chouioia cunea upon Exposure to 1-Dodecene

Cited by 5 publications

References 42 publications

Involvement of Chemosensory Protein CrufCSP3 in Perception of the Host Location in a Parasitic Wasp Cotesia ruficrus

Involvement of Chemosensory Protein CrufCSP3 in Perception of the Host Location in a Parasitic Wasp Cotesia ruficrus

A full-length transcriptome and gene expression analysis of three detoxification gene families in a predatory stink bug, Picromerus lewisi

Characterization of full-length transcriptome in Saccharum officinarum and molecular insights into tiller development

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