Full-length transcriptional analysis reveals the complex relationship of leaves and roots in responses to cold-drought combined stress in common vetch

Abstract

Plant responses to single or combined abiotic stresses between aboveground and underground parts are complex and require crosstalk signaling pathways. In this study, we explored the transcriptome data of common vetch (Vicia sativa L.) subjected to cold and drought stress between leaves and roots via meta-analysis to identify the hub abiotic stress-responsive genes. A total of 4,836 and 3,103 differentially expressed genes (DEGs) were identified in the leaves and roots, respectively. Transcriptome analysis results showed that the set of stress-responsive DEGs to concurrent stress is distinct from single stress, indicating a specialized and unique response to combined stresses in common vetch. Gene Ontology (GO) enrichment analyses identified that “Photosystem II,” “Defence response,” and “Sucrose synthase/metabolic activity” were the most significantly enriched categories in leaves, roots, and both tissues, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results indicated that “ABC transporters” are the most enriched pathway and that all of the genes were upregulated in roots. Furthermore, 29 co-induced DEGs were identified as hub genes based on the consensus expression profile module of single and co-occurrence stress analysis. In transgenic yeast, the overexpression of three cross-stress tolerance candidate genes increased yeast tolerance to cold-drought combined stress. The elucidation of the combined stress-responsive network in common vetch to better parse the complex regulation of abiotic responses in plants facilitates more adequate legume forage breeding for combined stress tolerance.

Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola

“…SMRT sequencing produces longreads and is particularly useful for non-model species for which whole-genome sequencing data are lacking [33,34]. With the continuous improvement of sequencing technology, the application of third-generation sequencing technology in the study of plant interaction is also very common [35][36][37]. Here, our results provide the first comprehensive, high-quality, full-length transcriptome of the sesame cultivars 'Henan No.1' and 'Jinzhi No.3' via a combination of SMRT-seq and RNA-seq, with correction of SMRT reads using Illumina reads.…”

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

“…Seed germination and seedling emergence are important stages in the establishment of a plant species and the most sensitive ones in the plant's life cycle under environmental conditions [47]. In the context of global climate change, drought, salinity, and cold are the major adverse factors limiting seed germination and early establishment as well as subsequent growth and productivity [48][49][50]. In addition, burial depth is also one of the primary factors known to influence seedling emergence and establishment [51], and deep sowing resulted in mechanical stress on seeds and seedlings, which ultimately caused poor emergence performance and stand establishment [52].…”

Surviving a Double-Edged Sword: Response of Horticultural Crops to Multiple Abiotic Stressors

“…Additionally, studies have reported how horticultural crops respond to combined stresses containing drought and salt [43,44], drought and cold [45], etc. Despite the importance, studies are lacking and need attention to address the complex mechanisms underlying these stresses.…”