Full length nucleotide sequence of foot-and-mouth disease virus strain O 1 Campos/Bra/58

Pereda, Ariel; König, Guido; Zoth, Silvina Andrea Chimeno; Borca, Manuel V.; Palma, Eduardo L.; Piccone, María E.

doi:10.1007/s00705-002-0872-9

Cited by 14 publications

(4 citation statements)

References 19 publications

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“…FMDV genomes ranged from 8,046 to 8,215 nt, consistent with previously reported genome lengths (86,108). Although small insertions/deletions were observed in the coding region affecting L pro , 1B, 1C, 1D, and 3A, most variability in genome size was due to insertions/deletions in the UTRs.…”

Section: Resultssupporting

confidence: 87%

“…Comparative genomic analysis of a large number of FMDV genomes may allow the identification of highly conserved regulatory or coding regions which are critical for aspects of virus biology. To date, the few comparative analyses of full-length FMDV genomes have relied on intratypic (serotype O) or intertypic (serotypes O, A, and C) comparisons of a small number of isolates and serotypes (70,86). In addition, complete genomic sequences of some serotypes are not available (SAT1 and SAT3) or, for others, only represent highly cell culture-adapted isolates (serotypes A and C).…”

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confidence: 99%

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Comparative Genomics of Foot-and-Mouth Disease Virus

Carrillo

Tulman

Delhon

et al. 2005

J Virol

442

479

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Here we present complete genome sequences, including a comparative analysis, of 103 isolates of foot-andmouth disease virus (FMDV) representing all seven serotypes and including the first complete sequences of the SAT1 and SAT3 genomes. The data reveal novel highly conserved genomic regions, indicating functional constraints for variability as well as novel viral genomic motifs with likely biological relevance. Previously undescribed invariant motifs were identified in the 5 and 3 untranslated regions (UTR), as was tolerance for insertions/deletions in the 5 UTR. Fifty-eight percent of the amino acids encoded by FMDV isolates are invariant, suggesting that these residues are critical for virus biology. Novel, conserved sequence motifs with likely functional significance were identified within proteins L pro , 1B, 1D, and 3C. An analysis of the complete FMDV genomes indicated phylogenetic incongruities between different genomic regions which were suggestive of interserotypic recombination. Additionally, a novel SAT virus lineage containing nonstructural proteinencoding regions distinct from other SAT and Euroasiatic lineages was identified. Insights into viral RNA sequence conservation and variability and genetic diversity in nature will likely impact our understanding of FMDV infections, host range, and transmission.

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Section: Resultssupporting

confidence: 87%

mentioning

confidence: 99%

Comparative Genomics of Foot-and-Mouth Disease Virus

Carrillo

Tulman

Delhon

et al. 2005

J Virol

442

479

View full text Add to dashboard Cite

show abstract

“…Indeed, data have been biased by the phylogenetic nature of the analyses conducted (reviewed in reference 24). The number of studies utilizing full-length FMDV genomes for comparative evolutionary analyses has been even less significant and mainly concentrated on intra-or intertypic comparisons of a limited number of isolates (27,36). These studies have been also biased by the fact that only a few serotypes have been considered (notably O, A, and C) due to the lack of resolution of full-length genomes for some of the serotypes, especially for the sub-Saharan serotypes SAT1 and SAT3.…”

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confidence: 99%

Shifts in the Selection-Drift Balance Drive the Evolution and Epidemiology of Foot-and-Mouth Disease Virus

Tully

Fares

2009

J Virol

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Foot-and-mouth disease virus (FMDV) is the causative agent of an acute vesicular disease affecting wild and domesticated animals. Despite the economic burden of the disease and all efforts to eradicate it, FMD outbreaks continue to emerge unexpectedly in developed and developing countries. Correlation of the mutational dynamics of the virus with its epidemiology remains unexplored. Analysis of 103 complete genomes representing the seven serotypes shows the important role that selection plays in the genomic evolution of viral isolates for serotypes. We identified selection and relaxed constraints due to genetic drift through analyses of synonymous sites. Finally, we investigated interactions between mutations that showed coevolving patterns and analyzed, based on protein structures, slightly deleterious and compensatory mutational dynamics. Specifically, we demonstrate that structurally exposed capsid proteins present a greater number of adaptive mutations and relaxed selection than nonstructural proteins. Such events have been magnified during the evolution of the southern African virus types (SATs). These shifts in selection-drift balance have generated the great antigenic and genetic diversity observed for SAT serotypes and that are responsible for epizootics on the continent of Africa. The high number of slightly deleterious and compensatory mutations in SAT serotypes in structural proteins is testament to such balance plasticity. The significant accumulation of these coevolving mutations in African serotypes supports their contribution in generating adaptive immune-escaping mutants and in establishing persistent infections. The reverse of this pattern in nonstructural proteins reveals the neutral fixation of mutations in the more widely spread and commonly studied Euro-Asiatic serotypes.

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“…9 Briefly, recombinant proteins to the NSPs 3A, 3B, 3ABC, and 3D were produced by cloning PCR-amplified DNA fragments from cDNA of the FMDV strain O 1 /Campos/Brazil/58. 28 The proteins were each covalently coupled to a unique carboxylate bead class (Luminex Corp.) using the carbodiimide activation as described below.…”

Section: Methodsmentioning

confidence: 99%

Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus

et al. 2006

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Liquid array technology was used to develop a multiplexed assay for the detection of antibodies to viral nonstructural proteins (NSPs), raised in cattle in response to infection with foot-and-mouth disease (FMD) virus. Two assays, one based on recombinant NSPs and the other on synthetically produced peptides, were developed and compared side-by-side. Serum samples from serial bleeds of cattle, each experimentally infected with one of the seven serotypes (C, A, O, Asia, SAT1, SAT2, SAT3) of FMD virus were analyzed. A distinct pattern in the detection of NSP antibodies and a close correlation of the recombinant protein and peptide-based assays were observed. The detection of antibodies to NSPs is a method to differentiate FMD-infected and FMD-vaccinated animals, and a high-throughput assay would be an invaluable tool in the case of an outbreak of FMD in North America, when emergency vaccination may be utilized to spare vaccinated, noninfected animals from slaughter and subsequent disposal.

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Full length nucleotide sequence of foot-and-mouth disease virus strain O 1 Campos/Bra/58

Cited by 14 publications

References 19 publications

Comparative Genomics of Foot-and-Mouth Disease Virus

Comparative Genomics of Foot-and-Mouth Disease Virus

Shifts in the Selection-Drift Balance Drive the Evolution and Epidemiology of Foot-and-Mouth Disease Virus

Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus

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