Full genome analysis of <scp>P</scp>hilippine indigenous subgenotype <scp>IA</scp> hepatitis <scp>A</scp> virus strains from <scp>J</scp>apanese patients with imported acute hepatitis <scp>A</scp>

Watanabe, Shunji; Isoda, Norio; Ohtake, Toshiya; Hirosawa, Takuya; Morimoto, Naoki; Aoki, K. Roger; Takahashi, Masaharu; Sugano, Kentaro; Okamoto, Hiroaki

doi:10.1111/hepr.12124

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…Three HAV isolates of Cluster 1 shared 98.5–98.7% identities over the entire genome, while the mean identities between the four distinct clusters were 96.2–97.3%, further supporting the validity of the clustering (Supplementary Table SIV). As shown in Figure , the phylogenetic tree constructed based on the entire genome also confirmed that the six Mongolian HAV isolates of the four clusters obtained in the present study are classifiable into subgenotype IA and further into an Asian IA lineage, consisting of HAV strains from China, Indonesia, Japan, Malaysia, Papua New Guinea, Philippines, Singapore, and Thailand [Fujiwara et al, ; Hu et al, ; Watanabe et al, ; Mulyanto et al, ; Ishii et al, ], with a bootstrap value of 100%. This tree also revealed that the polyphyletic IA strains are circulating in Mongolia.…”

Section: Resultssupporting

confidence: 79%

Molecular analysis of hepatitis A virus strains obtained from patients with acute hepatitis A in Mongolia, 2004–2013

Tsatsralt-Od

Baasanjav

Dulmaa

et al. 2015

Journal of Medical Virology

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Despite the high endemicity of hepatitis A virus (HAV) in Mongolia, the genetic information on those HAV strains is limited. Serum samples obtained from 935 patients with acute hepatitis in Ulaanbaatar, Mongolia during 2004-2013 were tested for the presence of HAV RNA using reverse transcription-PCR with primers targeting the VP1-2B region (481 nucleotides, primer sequences at both ends excluded). Overall, 180 patients (19.3%) had detectable HAV RNA. These 180 isolates shared 94.6-100% identity and formed four phylogenetic clusters within subgenotype IA. One or three representative HAV isolates from each cluster exhibited 2.6-3.9% difference between clusters over the entire genome. Cluster 1 accounted for 65.0% of the total, followed by Cluster 2 (30.6%), Cluster 3 (3.3%), and Cluster 4 (1.1%). Clusters 1 and 2 were predominant throughout the observation period, whereas Cluster 3 was undetectable in 2009 and 2013 and Cluster 4 became undetectable after 2009. The Mongolian HAV isolates were closest to those of Chinese or Japanese origin (97.7-98.5% identities over the entire genome), suggesting the evolution from a common ancestor with those circulating in China and Japan. Further molecular epidemiological analyses of HAV infection are necessary to investigate the factors underlying the spread of HAV and to implement appropriate prevention measures in Mongolia.

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Section: Resultssupporting

confidence: 79%

Molecular analysis of hepatitis A virus strains obtained from patients with acute hepatitis A in Mongolia, 2004–2013

Tsatsralt-Od

Baasanjav

Dulmaa

et al. 2015

Journal of Medical Virology

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“…Although the IIIA outbreak in the Tohoku region was restricted to a small region from week 5 to 7 and subsequently replaced by 2014JapanIA-EPM, we must acknowledge the possibility that the subgenotype IIIA strain has also recently become a locally circulating strain in Japan. Previously, we found that the multi-location outbreak of hepatitis A in the spring of 2010 was caused not only by locally circulating strains, but also by two HAV strains that may be related to Philippines (IA-2) and Korea (IIIA) strains [13,27]. The 2010 HAV outbreak can be attributed to introduction of HAV strains from two newly emerged lineages that were relatively rare in Japan until 2009 [28,29].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological and genetic analysis of a 2014 outbreak of hepatitis A in Japan

Ishii

Kiyohara

Yoshizaki

et al. 2015

Vaccine

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“…These strains were close to 2 Japanese HAV strains, AB793726 and AB973401, with nucleotide sequence identities of 96.5%-98.8% and 97.4%-99.1%, respectively. Strain AB793726 was detected in a Japanese patient in 2012, and it is considered to have been imported from the Philippines (12). Strain AB973401 was isolated from a Japanese patient during a mini-epidemic in 2010 and has a nucleotide sequence that is identical to that of strain EU825856, which was isolated from a German patient who developed acute hepatitis after traveling to the Philippines (13).…”

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confidence: 99%

Detection of Subgenotype IA and IIIA Hepatitis A Viruses in Rivers Flowing through Metro Manila, the Philippines

et al. 2019

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Hepatitis A virus (HAV) is a common infectious etiology of acute hepatitis worldwide. The Philippines remains highly endemic for hepatitis A, but there is still lack of information about HAV in the Philippines. To understand the contamination of environment water with HAV in the Philippines, we performed detection and genetic analyses of HAV RNA using samples from the river water. A total of 12 water samples were collected at six sampling sites of three rivers in both dry and wet seasons in Metro Manila in 2012 to 2013. The HAV RNA was detected from all six samples collected in the dry season and from one sample in the wet season. The phylogenetic analysis confirmed that the HAV strains detected in the river water included multiple HAV sequences belonging to subgenotype IA and IIIA, indicating that at least two genotypes of the HAV strains are circulating in environment in the Philippines, posing a risk of HAV infection to not only residents but also tourists, especially in the dry season.

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Full genome analysis of Philippine indigenous subgenotype IA hepatitis A virus strains from Japanese patients with imported acute hepatitis A

Abstract: These results indicate that HAV isolates belonging to the IA-2 lineage might have been imported from the Philippines.

Cited by 8 publications

References 23 publications

Molecular analysis of hepatitis A virus strains obtained from patients with acute hepatitis A in Mongolia, 2004–2013

Molecular analysis of hepatitis A virus strains obtained from patients with acute hepatitis A in Mongolia, 2004–2013

Epidemiological and genetic analysis of a 2014 outbreak of hepatitis A in Japan

Detection of Subgenotype IA and IIIA Hepatitis A Viruses in Rivers Flowing through Metro Manila, the Philippines

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