Full Genome Analysis of Influenza A(H1N1)pdm09 Virus Isolated from Peru, 2013

Padilla‐Rojas, Carlos; Condori, F; Huaringa, Maribel; Marcos-Carbajal, Pool; Rojas, Nancy; Gutierrez, Victoria; Cáceres, Omar

doi:10.1128/genomea.00191-14

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…This transient mutation, also called an ''adaptive mutation'', may be the consequence of individual mutations that relieve the selective pressure caused by survival [11]. The K180Q mutation was (at least, in the Beijing area) a novel substitution within the Sa antigenic site of the H1 segment; this mutation has also been observed in Peru and India since 2013 [9,26]. In H3, three amino acid mutations were detected at two antigenic sites (A and B); these substitutions were observed in 2012-2013, but at a lower frequency [10].…”

Section: Discussionmentioning

confidence: 93%

Molecular epidemiology and evolution of influenza A and B viruses during winter 2013-2014 in Beijing, China

et al. 2015

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In this study, we investigated the molecular epidemiology and evolution of influenza viruses from patients infected during the 2013-2014 influenza season in Beijing. A phylogenetic analysis of the hemagglutinin (HA) and neuraminidase (NA) sequences of influenza A and B viruses from 18 patients (6 A(H1N1)pdm09, 4 H3N2, and 8 influenza B virus) was performed. Among the influenza A viruses, A(H1N1)pdm09 was the dominant subtype, whereas the B/Yamagata lineage was predominant for influenza B. The influenza B HA and NA strains in Beijing were dominated by reassortants derived from the Yamagata lineage and the Victoria lineage, respectively. All six A(H1N1)pdm09 strains fell into the 6B genetic group with amino acid substitutions D97N, S185T, K163Q, and A256T; the four H3N2 strains fell into genetic group 3C.3 with substitutions T128A, R142G, N145S, and V186G, and the eight influenza B strains were categorized into subgroup 3.1 and harbored an N217S mutation. Two new mutations (K180Q and G187E at the Sa and Ca antigenic sites of the H1 segment, respectively), which were not detected during the preceding influenza season, were identified. Mutations N131K, S165I, N181Y, and D212N in HA of influenza B mapped to the 120-loop, 150-loop, 160-loop, and 190-helix, respectively. Our results reveal the molecular epidemiology and phylogenetic characteristics of influenza viruses within a single geographic location and can have implications for vaccination selection in northern China.

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Section: Discussionmentioning

confidence: 93%

Molecular epidemiology and evolution of influenza A and B viruses during winter 2013-2014 in Beijing, China

et al. 2015

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“…This mutant was first described in isolates from Peru, and our analysis showed that its location within the antigenic site Sa could allow the virus to escape from the immune response elicited by current vaccines. 22 Moreover, this change has been described in escape mutants of the HA from 1918. 23 Interestingly, despite that mutant S188T is located in antigenic site Sb and has become prevalent since 2009, 24 it appears to have had little impact in antigenic drift because no evidence has been reported to date of reduced vaccine efficacy caused by this mutant.…”

Section: Discussionmentioning

confidence: 95%

The hemagglutinin of the influenza A(H1N1)pdm09 is mutating towards stability

Castelán-Vega¹,

Magaña-Hernández²,

Jiménez-Alberto³

et al. 2014

AABC

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The last influenza A pandemic provided an excellent opportunity to study the adaptation of the influenza A(H1N1)pdm09 virus to the human host. Particularly, due to the availability of sequences taken from isolates since the beginning of the pandemic until date, we could monitor amino acid changes that occurred in the hemagglutinin (HA) as the virus spread worldwide and became the dominant H1N1 strain. HA is crucial to viral infection because it binds to sialidated cell-receptors and mediates fusion of cell and viral membranes; because antibodies that bind to HA may block virus entry to the cell, this protein is subjected to high selective pressure. Multiple alignment analysis of sequences of the HA from isolates taken since 2009 to date allowed us to find amino acid changes that were positively selected as the pandemic progressed. We found nine changes that became prevalent: HA1 subunits D104N, K166Q, S188T, S206T, A259T, and K285E; and HA2 subunits E47K, S124N, and E172K. Most of these changes were located in areas involved in inter- and intrachain interactions, while only two (K166Q and S188T) were located in known antigenic sites. We conclude that selective pressure on HA was aimed to improve its functionality and hence virus fitness, rather than at avoidance of immune recognition.

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“…Also, HRM analysis for characterization of influenza subtypes [18, 25-27] and resistance isolates were reported [10-12,[28-31]. In this study, HRM analysis allowed us to analyze a larger number of samples compared to similar previous studies [32-33], which allowed us to obtain more robust data on the distribution of the most frequent genotypes circulating in Peru. Likewise, our results can be used to monitor changes in the distribution of influenza A(H1N1)pdm09 genotypes over time, like that reported in other studies [7-8,[19-22].…”

Section: Resultsmentioning

confidence: 90%

Characterization of influenza A(H1N1)pdm09 isolates of Peru using HRM, a post PCR molecular biology method

et al. 2019

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Influenza caused by A(H1N1)pdm09 is a public health issue with severe conditions in vulnerable populations leading to death. Therefore, it is of interest to characterize and monitor influenza A(H1N1)pdm09 genotypes using High Resolution Melting (HRM), a post PCR molecular biology method. We used HRM analysis (using RotorGene Q thermocycler) to characterize A(H1N1)pdm09 genotypes from several places of Peru. RNA was purified from nasal and pharyngeal swab samples referred to LRNVR-INS, synthesized cDNA, and then the hemagglutinin gene and matrix fragment were amplified. Thus, 287 samples positive for influenza A(H1N1)pdm09 were identified across Peru where places like Lima, Piura, and Arequipa documented highest number of cases. The HRM data was analyzed and results showed different profiles which were further grouped into four genotypes for the HA (A, B, C, D) and 3 for the M (a, b, c) genes. We also report ten genotypes (I-X) of virus using combined HA (hemagglutinin) and M gene profiles representing a national geography. The prevalent genotypes are I and II with a frequency of 35.89% (103) and 29.27% (84), respectively linking with severe acute respiratory infection.

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Full Genome Analysis of Influenza A(H1N1)pdm09 Virus Isolated from Peru, 2013

Cited by 3 publications

References 5 publications

Molecular epidemiology and evolution of influenza A and B viruses during winter 2013-2014 in Beijing, China

Molecular epidemiology and evolution of influenza A and B viruses during winter 2013-2014 in Beijing, China

The hemagglutinin of the influenza A(H1N1)pdm09 is mutating towards stability

Characterization of influenza A(H1N1)pdm09 isolates of Peru using HRM, a post PCR molecular biology method

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