Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas

Ghouali, W; Grieve, Kate; Bellefqih, S.; Sandali, Otman; Harms, Fabrice; Laroche, L.; Pâques, Michel; Borderie, Vincent

doi:10.3109/02713683.2014.935444

Cited by 42 publications

(42 citation statements)

References 22 publications

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“…It has been used for imaging of the retina, 4 cornea, [5][6][7] corneal limbus, 8 and other ophthalmic tissues, along with other biological tissues such as brain, 9 breast, 10,11 and gastrointestinal tissues. 12 The lack of the need for fixation and contrast agents in FFOCT is an advantage, particularly in longitudinal studies where a single retinal explant, for example, is to be imaged at multiple timepoints.…”

Section: Resultsmentioning

confidence: 99%

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Appearance of the Retina With Full-Field Optical Coherence Tomography

Grieve

Thouvenin

Sengupta

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Section: Resultsmentioning

confidence: 99%

“…The FFOCT system used in this study (LLTech, Paris, France) has been described previously. 7 Illumination is provided by a halogen source, whose short coherence length leads to an axial resolution of 1 lm. The full field is illuminated and images are captured on a complementary metal oxide semiconductor (CMOS) camera.…”

Section: Imaging Technologiesmentioning

confidence: 99%

Appearance of the Retina With Full-Field Optical Coherence Tomography

Grieve

Thouvenin

Sengupta

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…13 Image stacks at the conus for keratoconus and at the center for normals were acquired on each button covering the entire corneal thickness, with an image acquired every micron in depth. Features noted in SD-OCT and IVCM were located and matched in FFOCM acquisitions by the same operator.…”

Section: Methodsmentioning

confidence: 99%

“…This configuration allows for higher resolution than conventional OCT, on the order of 1 μm. FFOCM has been used to image ophthalmic tissues, including cornea, 13,14 limbus, 15 lens, and retina. 16 …”

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confidence: 99%

Imaging Microscopic Features of Keratoconic Corneal Morphology

Grieve

Georgeon

Andreiuolo

et al. 2016

Cornea

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Purpose:To search for gold-standard histology indicators using alternative imaging modalities in keratoconic corneas.Methods:Prospective observational case–control study. Fourteen keratoconic corneas and 20 normal corneas (10 in vivo healthy subjects and 10 ex vivo donor corneas) were examined. Images of corneas were taken by spectral domain optical coherence tomography (SD-OCT) and in vivo confocal microscopy (IVCM) before keratoplasty. The same removed corneal buttons were imaged after keratoplasty with full-field optical coherence microscopy (FFOCM) and then fixed and sent for histology. Controls consisted of normal subjects imaged in vivo with IVCM and donor corneas imaged ex vivo with FFOCM. Corneal structural changes related to pathology were noted with each imaging modality. Cell density was quantified by manual cell counting.Results:Keratoconus indicators (ie, epithelial thinning/thickening, cell shape changes, ferritin deposits, basement membrane anomalies, Bowman layer thinning, ruptures, interruptions, scarring, stromal modifications, and appearance of Vogt striae) were generally visible with all modalities. Additional features could be seen with FFOCM in comparison with gold-standard histology, particularly in the Bowman layer region, whereas the combination of SD-OCT plus IVCM detected 76% of those features detected in histology. Three-dimensional FFOCM imaging aided interpretation of two-dimensional IVCM and SD-OCT data. Basal epithelial cell and keratocyte densities were significantly lower in patients with keratoconus than those in normals (P < 0.0001).Conclusions:Structural and cellular assessment of the keratoconic cornea by means of either in vivo SD-OCT combined with IVCM or ex vivo FFOCM in both cross-sectional and en face views can detect as many keratoconus indicators as gold-standard histology.

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“…Most of the work performed to characterize the microstructure of the human cornea has been carried out by using various optical techniques, such as electron microscopy, circular polarization microscopy, full-field optical coherence tomography, small-angle light scattering and x-ray diffraction [5][6][7][8][9]. Multiphoton microscopy techniques, such as two-photon excited (auto) fluorescence (TPEF), and second and third harmonic generation (SHG and THG, respectively), can be an alternative to these traditional methods, since they provide intrinsic tissue optical sectioning capabilities combined with deep penetration and multimodality signals approach [10].…”

Section: Introductionmentioning

confidence: 99%

Translational label-free nonlinear imaging biomarkers to classify the human corneal microstructure

Lombardo

Merino

Loza-Álvarez

et al. 2015

Biomed. Opt. Express

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Diseases that affect the cornea can lead to severe vision loss and have tremendous social impact. These diseases are associated to deviations from normal structural order and orientation of collagen fibril bundles. Unfortunately, resolving non-invasively the corneal collagen structure is not possible to date. In this work, polarization sensitive second harmonic generation (pSHG) microscopy is used to obtain information with molecular specificity on microstructure of human corneas. This information is used to develop a set of label-free imaging biomarkers that were generated by means of a novel methodology based on mathematical tensorial calculus. The method is proven to be highly sensitive and robust. The use of these biomarkers permits accurate characterization of the anisotropic, depth-dependent, structural organization of corneal collagen fibril bundles without any a priori information. The method can be valuable to improve understanding of microstructural pathophysiological changes of the human cornea close to in vivo conditions. References and Links1. J. P. Whitcher, M. Srinivasan, and M. P. Upadhyay, "Corneal blindness: a global perspective," Bull. World Health Organ. 79(3), 214-221 (2001). 2. D. M. Maurice, "The structure and transparency of the cornea," J. Physiol. 136(2), 263-286 (1957). 3. K. M. Meek, S. J. Tuft, Y. Huang, P. S. Gill, S. Hayes, R. H. Newton, and A. J. Bron, "Changes in Collagen Orientation and Distribution in Keratoconus Corneas," Invest. Ophthalmol. Vis. Sci. 46(6), 1948-1956 (2005). 4. M. S. Rajan, "Surgical strategies to improve visual outcomes in corneal transplantation," Eye (Lond.) 28(2), 196-201 (2014). 5. Y. Komai and T. Ushiki, "The three-dimensional organization of collagen fibrils in the human cornea and sclera," Invest. Ophthalmol. Vis. Sci. 32(8), 2244-2258 (1991). 6. K. M. Meek and C. Boote, "The use of X-ray scattering techniques to quantify the orientation and distribution of collagen in the corneal stroma," Prog. Retin. Eye Res. 28(5), 369-392 (2009 526-534 (2015). 10. W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21(11), 1369-1377 (2003). 11. S. Brasselet, "Polarization-resolved nonlinear microscopy: application to structural molecular and biological imaging," Adv. 73(364), 699-705 (1978). 39. J. M. Bueno, E. J. Gualda, and P. Artal, "Adaptive optics multiphoton microscopy to study ex vivo ocular tissues," J. Biomed. Opt. 15(6), 066004 (2010). 40. J. M. Bueno, R. Palacios, M. K. Chessey, and H. Ginis, "Analysis of spatial lamellar distribution from adaptiveoptics second harmonic generation corneal images," Biomed. Opt. Express 4(7), 1006-1013 (2013). #239832Received 27 harmonic generation in tissues with confocal multiphoton microscopy," J.

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Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas

Abstract: FF-OCT is a powerful non-invasive imaging tool that allows detailed study of corneal structures. Images correlate well with conventional histology. Further studies should evaluate the benefit of this technique as a complement to current assessment methods of human donor corneas.

Cited by 42 publications

References 22 publications

Appearance of the Retina With Full-Field Optical Coherence Tomography

Appearance of the Retina With Full-Field Optical Coherence Tomography

Imaging Microscopic Features of Keratoconic Corneal Morphology

Translational label-free nonlinear imaging biomarkers to classify the human corneal microstructure

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